- Vertically- and horizontally-transmitted memories – the fading boundaries between regeneration and inheritance in planaria
Summary: In this hypothesis paper we re-evaluate canonical ideas about the interaction between developmental, genetic and evolutionary processes through the lens of planaria, an invertebrate model organism which challenges fundamental assumptions regarding reproduction.
- How great white sharks nourish their embryos to a large size: evidence of lipid histotrophy in lamnoid shark reproduction
Summary: Great white sharks (Carcharodon carcharias) nourish their embryos with a lipid-rich ‘milk’ secreted by uterine epithelium during the early gestation period, before the onset of oophagy.
- Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation
Summary: We developed a CRISPR/dCas9-DNMT3A fusion protein to repress the expression of endogenous genes in combination with multiple guide RNAs. This tool can help us elucidate the role of DNA methylation in normal development and disease.
- miR-20a regulates proliferation, differentiation and apoptosis in P19 cell model of cardiac differentiation by targeting Smoothened
Summary: Our findings may bring a new viewpoint into the mechanisms of cardiac abnormalities and suggest that miR-20a may be a potential new therapeutic target for congenital heart diseases.
- Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice
Summary: We demonstrate the production of CRISPR-Cas9-mediated knockout and knock-in mice using a recently developed ultra-superovulation technique to obtain greater numbers of oocytes compared with conventional methods.
- An antibody toolkit for the study of membrane traffic in Drosophila melanogaster
Summary: We report the generation and characterization of set of monoclonal and polyclonal antibodies for labeling the major compartments of the secretory and endocytic pathways in Drosophila melanogaster.
- PFA fixation enables artifact-free super-resolution imaging of the actin cytoskeleton and associated proteins
Summary: We show that proper PFA fixation allows high-quality super-resolution imaging of the actin cytoskeleton and can outperform gold-standard glutaraldehyde fixation for imaging of actin-binding proteins.
- The effect of foot posture on capacity to apply free moments to the ground: implications for fighting performance in great apes
Summary: We tested the capacity of the heel-down foot posture of great apes to apply free moments to the ground because this could influence performance when fighting from a bipedal stance.
- Estimating energetics in cetaceans from respiratory frequency: why we need to understand physiology
Summary: Accounting for changes in tidal volume and gas exchange improves the ability to estimate field metabolic rate from respiratory frequency in cetaceans.
- 3D for the people: multi-camera motion capture in the field with consumer-grade cameras and open source software
Summary: Argus is a free and open source toolset for using consumer grade cameras to acquire 3D kinematic data in field settings.