A zebrafish model of inflammatory lymphangiogenesis

ABSTRACT Inflammatory bowel disease (IBD) is a disabling chronic inflammatory disease of the gastrointestinal tract. IBD patients have increased intestinal lymphatic vessel density and recent studies have shown that this may contribute to the resolution of IBD. However, the molecular mechanisms involved in IBD-associated lymphangiogenesis are still unclear. In this study, we established a novel inflammatory lymphangiogenesis model in zebrafish larvae involving colitogenic challenge stimulated by exposure to 2,4,6-trinitrobenzenesulfonic acid (TNBS) or dextran sodium sulphate (DSS). Treatment with either TNBS or DSS resulted in vascular endothelial growth factor receptor (Vegfr)-dependent lymphangiogenesis in the zebrafish intestine. Reduction of intestinal inflammation by the administration of the IBD therapeutic, 5-aminosalicylic acid, reduced intestinal lymphatic expansion. Zebrafish macrophages express vascular growth factors vegfaa, vegfc and vegfd and chemical ablation of these cells inhibits intestinal lymphatic expansion, suggesting that the recruitment of macrophages to the intestine upon colitogenic challenge is required for intestinal inflammatory lymphangiogenesis. Importantly, this study highlights the potential of zebrafish as an inflammatory lymphangiogenesis model that can be used to investigate the role and mechanism of lymphangiogenesis in inflammatory diseases such as IBD.


INTRODUCTION
Crohn's disease and ulcerative colitis are characterised as chronic inflammatory disorders of the gastrointestinal tract and together are known as inflammatory bowel disease (IBD). It is well accepted that the dysregulation of normally controlled immune responses to gut microbiota, as well as genetic susceptibility and other environmental factors contribute to the pathogenesis of IBD (Zhang and Li, 2014), however the aetiology of IBD is still not well understood.
Increased intestinal lymphatic vessel density is observed in IBD patients (Fogt et al., 2004;Geleff et al., 2003;Kaiserling et al., 2003;Pedica et al., 2008;Rahier et al., 2011) and in mouse models of IBD (D'Alessio et al., 2014;Ganta et al., 2010;Jurisic et al., 2013). Although increased lymphangiogenesis is proposed to occur in response to the decreased mesenteric lymphatic function observed in IBD patients (Heatley et al., 1980;Van Kruiningen and Colombel, 2008;Van Kruiningen et al., 2014), the mechanism by which these lymphatic vessels form is still unclear. The growth of lymphatic vessels during early lymphatic development largely involves the sprouting of lymphatic endothelial cells from the veins (Srinivasan et al., 2007;Yaniv et al., 2006) and requires vascular endothelial growth factor 3 (VEGFR-3) signalling (Irrthum et al., 2000;Karkkainen et al., 2000Karkkainen et al., , 2001Veikkola et al., 2001). The binding of extracellular VEGF ligands, in particular VEGF-C and VEGF-D, activates VEGFR-3 (Karkkainen et al., 2004;Kukk et al., 1996). Reducing inflammatory lymphangiogenesis using a VEGFR-3 blocking antibody, or decreasing lymphatic function through the down regulation of FoxC2, a gene required for lymphatic homeostasis, significantly increases inflammatory oedema and inhibits disease resolution in mouse models of IBD, suggesting a protective role of intestinal lymphatics in IBD (Becker et al., 2015;Jurisic et al., 2013). In agreement, a recent study has shown that enhancing lymphangiogenesis and lymphatic function, by adenoviral induction of VEGF-C, alleviates experimental chronic intestinal inflammation by increasing inflammatory cell mobilisation and bacterial antigen clearance (D'Alessio et al., 2014). Although these studies suggest there is potential in treating IBD by improving intestinal lymphatic function, further studies are required to determine the mechanism of IBD-associated lymphangiogenesis.
In other inflammatory situations such as chronic airway inflammation, skin inflammation, keratitis, and peritonitis, macrophages express pro-lymphatic growth factors, such as VEGF-A, VEGF-C, and VEGF-D, and macrophage depletion results in reduced inflammatory lymphangiogenesis (Baluk et al., 2005;Cursiefen et al., 2004;Harvey and Gordon, 2012;Kataru et al., 2009;Kim et al., 2009;Kubota et al., 2009;Maruyama et al., 2005;Tan et al., 2014;Zhang et al., 2007). A recent study has shown that neutrophils can stimulate skin inflammation-associated inflammatory lymphangiogenesis by increasing VEGF-A bioavailability, and also by secreting VEGF-D (Tan et al., 2013). Intestinal epithelial cells have also been shown to express VEGF-C (Joory et al., 2006). Therefore, it is possible that macrophages, neutrophils, and intestinal epithelial cells may contribute to IBD-associated lymphangiogenesis by secreting VEGF-A, VEGF-C, and VEGF-D. The availability of zebrafish transgenic lines that mark lymphatic vessels (Gordon et al., 2013;Okuda et al., 2012;van Impel et al., 2014), leukocytes (Ellett et al., 2011;Hall et al., 2007), and the intestinal epithelial cells (Her et al., 2004) makes the zebrafish model an ideal platform to uncover the in vivo interaction between these cell populations and lymphatic vessels in IBD.
Zebrafish are also gaining standing as a model for lymphatic development and, importantly, the requirement for Vegfr3/Vegfc signalling in lymphatic development is conserved in zebrafish (Hogan et al., 2009;Kuchler et al., 2006;Le Guen et al., 2014;Okuda et al., 2012;Yaniv et al., 2006). Combining existing zebrafish IBD and lymphatic models may therefore provide a novel platform for IBD-associated lymphangiogenesis research. In this study, we show that intestinal inflammation triggered by exposure of zebrafish larvae to TNBS or DSS resulted in Vegfr-dependent lymphangiogenesis, specifically in the zebrafish intestine. Furthermore, we show that zebrafish leukocytes express vascular growth factors and that macrophages are required for inflammatory lymphangiogenesis following colitogenic challenge.

Colitogenic challenge using TNBS or DSS stimulates development of lymphatic sprouts in the intestine
The aim of this study was to establish an inflammatory lymphangiogenesis model in zebrafish. To do this, we utilised the lyve1:DsRed2;kdrl:EGFP compound transgenic line which labels both the lymphatic/venous (lyve1) and blood (kdrl) vasculature and can therefore be used to differentiate the lymphatic vessels from the blood vessels (Okuda et al., 2012). Previous characterisation of this line has revealed that zebrafish develop an intestinal lymphatic (IL) network (summarised in supplementary material Fig. S1) (Okuda et al., 2012). Intestinal lymphatic sprouts (ILSs) (previously described as lymphatic branches) grow between the major intestinal lymphatic vessels and are rare on the left side of the zebrafish intestine at 7 days post-fertilisation (dpf). From 7 dpf, the number and length of the ILS steadily increases until 15 dpf, where they are hypothesised to contribute to the web-like IL that forms across the intestinal bulb (Okuda et al., 2012). The lack of ILSs on the left side of the larvae at 7 dpf provides a physiologically-relevant opportunity to identify and quantify intestinal lymphatic network expansion in intestinal disease.
When intestinal inflammation was induced using either TNBS or DSS in 3 dpf lyve1:DsRed2;kdrl:EGFP embryos, the number and total length of the ILSs were increased at 7 dpf when compared with untreated larvae (Fig. 1). Increased lymphangiogenic activity was intestine-specific, as no ectopic lymphatic vessels were observed in the trunk (Fig. 1A‴-C‴) or the head (data not shown). The lymphatic vessels induced following TNBS or DSS treatment predominately grew over the outer surface of the intestinal epithelium. However, there were rare examples where these ILS grew towards the swim bladder, dorsal to the intestine. Blood vessel development in the intestine also appeared normal in TNBS and DSS-treated larvae ( Fig. 1A-C). To show that the increased ILS formation was inflammation-dependent, we suppressed inflammation in TNBS or DSS treated larvae by co-administration of the anti-inflammatory drug 5-aminosalicylic acid (5-ASA) which is used to treat IBD (Rutgeerts et al., 2009). With 5-ASA co-treatment, the number and total length of the ILSs was reduced when compared with larvae exposed to colitogenic agents alone ( Fig. 1D-G), showing that TNBS/ DSS-driven lymphangiogenesis in the intestine is associated with inflammation. We therefore termed this model zebrafish intestinal inflammatory lymphangiogenesis (IIL).

5-ASA reduces neutrophil and macrophage recruitment to the intestine
Given that macrophages and neutrophils are known to contribute to inflammatory lymphangiogenesis in mammalian models (Tan et al., 2014), we next investigated whether reduction of zebrafish IIL following 5-ASA treatment was associated with decreased immune cell recruitment to the intestine. Using the neutrophil-specific lysozyme C promoter driven Tg(lyz:EGFP) transgenic line (Brannon et al., 2009;Hall et al., 2007) we confirmed our previous study showing that TNBS exposure results in an increase in neutrophil recruitment to the zebrafish intestine and that treatment with 5-ASA suppresses this (Oehlers et al., 2011) (see supplementary material Fig. S2). To establish whether TNBS treatment also altered macrophage recruitment to the intestine, larvae expressing the macrophage-specific macrophage expressed gene 1 promoter driven Tg(mpeg1:EGFP) transgene (Ellett et al., 2011) were treated at 3 dpf with TNBS and the number of mpeg1-positive, macrophagelineage cells in the intestine assessed at 7 dpf. The number of macrophages in the intestine of TNBS-treated larvae increased 2.2-fold when compared with untreated larvae (untreated, 34±12; TNBS-treated, 73±18) ( Fig. 2A,B,H). Confocal live imaging of double transgenic mpeg1:EGFP;intestinal fatty acid binding protein (i-fabp):RFP (Her et al., 2004) larvae demonstrated that macrophages accumulated around the outer surface of the i-fabpexpressing intestinal epithelial cells following TNBS treatment ( Fig. 2D-G). The total number of EGFP-positive macrophages in mpeg1:EGFP larvae, quantified by fluorescence activated cell sorting (FACS), increased 1.5-fold following TNBS treatment (untreated, 68/100,000±10; TNBS-treated, 99/100,000±10) (Fig. 2I). From this data we conclude that TNBS induces a systemic inflammatory response that is particularly severe in the intestine. Finally, 5-ASA suppressed the recruitment of macrophages to the zebrafish intestine in larvae exposed to TNBS; larvae treated with 5-ASA+TNBS had less intestinal macrophages when compared with TNBS-treated larvae ( Fig. 2A-C,H). Taken together these results show that 5-ASA co-treatment reduces TNBS-mediated inflammation in the zebrafish intestine.

Zebrafish macrophages and neutrophils express lymphatic growth factors
RT-PCR was used to investigate whether zebrafish larval macrophages express lymphatic growth factors. Macrophage-lineage cells were isolated from zebrafish larvae by FACS of EGFPpositive cells from the mpeg1:EGFP transgenic at 7 dpf. The isolated population was subsequently confirmed to be macrophages as cells strongly expressed the macrophage marker, colony stimulating factor 1 receptor a (csf1ra) with little or no expression of the neutrophil marker, myeloperoxidase (mpx) or the intestinal marker, caudal type homeobox 1 b (cdx1b) (Fig. 3A) (Flores et al., 2008;Meijer et al., 2008). We found that zebrafish macrophagelineage cells expressed vegfaa, vegfc, and vegfd in both untreated and TNBS-treated larvae (Fig. 3A). A previous study has shown that the expression level of vegfc in macrophages increases in a mouse model of skin inflammation, contributing to the increased VEGF-C concentration in the inflamed site . To investigate if this occurs in zebrafish IBD, the expression level of vegfc in zebrafish macrophages isolated from TNBS-treated larvae was compared with that of untreated larvae using qPCR and was shown to remain unchanged (Fig. 3B). To investigate whether zebrafish neutrophils express lymphatic growth factors, zebrafish neutrophils were isolated by collecting EGFP-positive cells from the lyz:EGFP transgenic using FACS at 7 dpf. The isolated cell population strongly expressed mpx, with little or no expression of csf1ra and cdx1b (Fig. 3C), consistent with a neutrophil phenotype. Zebrafish neutrophils were found to express vegfaa and vegfd in both untreated and TNBS-treated larvae, however, we did not consistently obtain an RT-PCR product for vegfc (Fig. 3C), suggesting that zebrafish neutrophils may only express low levels of vegfc mRNA.

Zebrafish intestinal epithelial cells express lymphatic growth factors
Epithelial cells are a source of VEGF-A (Gong and Koh, 2010;Halin et al., 2007) and VEGF-C (Baluk et al., 2005) in mouse models of inflammatory diseases. We therefore hypothesised that intestinal epithelial cells may also be a source of lymphatic growth factors in our zebrafish IIL model. To investigate this, RFP-positive cells were isolated by FACS from the i-fabp:RFP transgenic line (Her et al., 2004), at 7 dpf, and the expression of lymphatic growth factors was studied using RT-PCR. The isolated cell population strongly expressed the intestinal marker cdx1b, with little or no expression of the leukocyte markers csf1ra and mpo, (see

The Vegfr pathway is required for zebrafish intestinal inflammatory lymphangiogenesis
As zebrafish macrophages and neutrophils express vegfaa/c/d, we next investigated whether the Vegfr pathway is required for zebrafish IIL. Two pan-VEGFR inhibitors were used: tivozanib (Buchanan et al., 2012;Nakamura et al., 2006) or sunitinib (Cao et al., 2008;Mendel et al., 2003). The efficacy of these inhibitors on lymphatic vessel development was determined by treating 3 dpf embryos with either 50 nM tivozanib or 3 μM sunitinib, which resulted in a lack of trunk and intestinal lymphatics at 7 dpf (see supplementary material Fig. S4). In our model, the ILS generated during IIL develop from the intestinal lymphatics, therefore we needed to reduce the dose of each inhibitor to allow the development of the intestinal lymphatic vessels. We chose to use a 20-fold lower dose of each inhibitor (2.5 nM tivozanib or 150 nM of sunitinib) as these doses were not sufficient to inhibit developmental lymphangiogenesis ( Fig. 4A-E, see supplementary material Fig. S4). Co-administration of either Vegfr inhibitor with TNBS to 3 dpf embryos resulted in significantly reduced IIL at 7 dpf when compared with the 1% DMSO and TNBS co-treatment vehicle control (Fig. 4A-E) suggesting that the Vegfr pathway is required for IIL in zebrafish.

Macrophages are required for zebrafish intestinal inflammatory lymphangiogenesis
To determine whether macrophages and/or neutrophils are required for IIL we utilised transgenic lines in which either macrophagelineage cells (mpeg1:Gal4FF;UAS:nfsB:mCherry) or neutrophils (mpx:KalTA4;UAS:nfsB:mCherry) co-express mCherry and the bacterial enzyme nitroreductase (nfsB). Following addition of metronidazole, nitroreductase-expressing cells are able to convert this pro-drug into a cytotoxic metabolite resulting in cell death (Curado et al., 2007;Pisharath et al., 2007). Compound lyve1:DsRed; kdrl;EGFP;mpeg1:Gal4FF;UAS:nfsB:mCherry and lyve1:DsRed; kdrl;EGFP;mpx:KalTA4;UAS:nfsB:mCherry transgenic lines were pre-treated with 2.5 mM metronidazole at 2.3 dpf, before exposure to a TNBS and metronidazole co-treatment from 3-7 dpf (Fig. 5A). This treatment regimen, achieved a 75% reduction in macrophage-lineage cells and a 65% reduction in neutrophils by 3 dpf and at 7 dpf, the numbers of both cell types were reduced by over 95% (Fig. 5G,H). IIL was significantly reduced following the ablation of mpeg1-expressing cells (Fig. 5C,D,I,J) but was not affected by the ablation of mpxexpressing cells (Fig. 5E,F,I,J) suggesting that macrophages but not neutrophils mediate inflammatory lymphangiogenesis in the zebrafish intestine following treatment with TNBS. Importantly, when lyve1:DsRed;kdrl;EGFP animals that did not express nitroreductase were exposed to 2.5 mM metronidazole and TNBS, we observed normal levels of IIL, showing that metronidazole alone does not inhibit inflammatory lymphangiogenesis (Fig. 5B,I,J). As haemogenic endothelium-derived cells have been implicated in developmental lymphangiogenesis (Klotz et al., 2015;Stanczuk et al., 2015) one explanation for the lack of IIL following macrophage ablation is that they are required for non-inflammatory, developmental lymphangiogenesis that is required to generate the parent intestinal lymphatic vessels for IIL to occur. To test this, we measured thoracic duct formation at 5 dpf and ILS length at 7 dpf following the ablation of macrophages and found that both were normal when mpeg1expressing cells were ablated in embryos that were not treated with TNBS (see supplementary material Fig. S5), suggesting that these cells are not required for normal lymphatic development in zebrafish and that macrophages have a specific role in inflammatory lymphangiogenesis.

DISCUSSION
In this study, we have shown that TNBS and DSS-induced colitogenic challenge results in increased ILS development, which we termed zebrafish IIL. We confirmed that zebrafish IIL was inflammatorydriven, as reduction of intestinal inflammation using the IBD therapeutic anti-inflammatory 5-ASA suppressed IIL. These results demonstrate that zebrafish exposed to DSS or TNBS can model the increased intestinal lymphangiogenesis observed in IBD patients (D'Alessio et al., 2014;Fogt et al., 2004;Geleff et al., 2003;Kaiserling et al., 2003;Pedica et al., 2008;Rahier et al., 2011). We could not determine if the increase in ILS number and length following TNBS/DSS treatment was due to ectopic ILS formation or from accelerated development of normal ILS formation; nevertheless, this is the first report demonstrating that zebrafish lymphatic vessels can respond to inflammatory stimulation and establishes a zebrafish model to study inflammatory-driven lymphatic growth. A recent study in mice has shown that lymph node lymphangiogenesis in Fig. 3. Zebrafish macrophages and neutrophils express lymphatic growth factors. (A) RT-PCR of vegfaa, vegfc, vegfd, csf1ra, mpx, cdx1b, and ef1-a from RNA isolated from untreated and TNBS-treated 7 dpf zebrafish macrophage-lineage cells. (B) qPCR analysis of vegfc mRNA in TNBStreated 7 dpf zebrafish macrophages relative to untreated 7 dpf zebrafish macrophages (n=3). (C) RT-PCR of vegfaa, vegfc, vegfd, csf1ra, mpx, cdx1b, and ef1-a from RNA isolated from untreated and TNBS-treated 7 dpf zebrafish neutrophils. The colours in the gel images are inverted and the numbers in brackets indicate reproducibility over three experiments. Error bars, ±s.d. n.s. P>0.05 by unpaired t-test.
response to oxazolone-induced inflammation can be visualised using a Prox1 lymphatic reporter line (Truman et al., 2012(Truman et al., , 2013. However, our zebrafish model of inflammatory lymphangiogenesis enables us to visualise the interactions between leukocytes, inflamed tissues, and lymphatic vessels in response to inflammatory stimuli in a live embryo. Furthermore, the optical transparency of zebrafish embryos allows time-lapse imaging of developing inflammatory-associated lymphatic vessels at single-cell resolution, which is currently still challenging in adult zebrafish and in mammalian models of IBD. While our data provides clear evidence implicating macrophages in IIL there are some limitations in our model that restrict the direct translation of our findings to human IBD. Firstly, this work was conducted in larval zebrafish in which the intestinal lymphatic vessels are still developing. Although we have ruled out a role for macrophages in developmental lymphangiogenesis, our findings should be validated in an adult model of IBD (Geiger et al., 2013) to ensure that similar mechanisms of inflammatory lymphangiogenesis occur in adult mesenteric lymphatics. However, these studies will be challenging due to the difficulty of live-imaging adult fish and because the anatomy of adult intestinal lymphatics has yet to be described in zebrafish. Second, our immersion model causes widespread inflammation whereas during IBD, inflammation is restricted to the intestine. While it is clear that intestinal inflammation is induced in our model, we cannot rule out the possibility that our IIL phenotype is due to non-intestinal inflammation. There are models of IBD in the fish that induce more localised intestinal inflammation that could be used to further substantiate our findings (Thakur et al., 2014).
The VEGFR pathway is essential for inflammatory lymphangiogenesis in various diseases, including IBD (D'Alessio et al., 2014;Jurisic et al., 2013;Kim et al., 2014). The application of Vegfr inhibitors tivozanib or sunitinib, prevented zebrafish IIL. Treatment with either 150 nm tivozanib or 3 μM sunitinib was sufficient to prevent IIL but at this concentration, developmental lymphangiogenesis in the intestine was inhibited, leading to a loss of the intestinal lymphatics and consequently no ILS could develop following TNBS treatment. Because of this, the concentration of both Vegfr inhibitors was titrated down to a level that allowed developmental lymphangiogenesis to occur but was still sufficient to prevent inflammatory lymphangiogenesis (tivozanib 5 nM, sunitinib 150 nM). These doses were however still 15-fold higher than the inhibitor IC 50 values for mammalian VEGFR-3; tivozanib 0.24 nM (Nakamura et al., 2006), sunitinib 10 nM (Mendel et al., 2003) suggesting that they are sufficient to inhibit Vegfr signalling and that this pathway has an essential role in our IIL model. However, the question that remained was what tissues or cells provide the VEGFs necessary for IIL to occur?
Intestinal epithelial cells express vegfaa and vegfc. This is similar to mammalian intestinal epithelial cells, which have been shown to express VEGF-A and VEGF-C (Griga et al., 2002(Griga et al., , 1999bJoory et al., 2006). Unlike the development of the zebrafish intersegmental vessels that require vegfaa expressed in the somites (Lawson et al., 2002; Liang et al., 1998;Nasevicius and Ekker, 2000), the source of Vegfs involved in zebrafish intestinal blood vessel development is still unknown. Given that intestinal epithelial cells express vegfaa and vegfc, they may contribute to developmental angiogenesis/ lymphangiogenesis in the zebrafish intestine by secreting Vegfaa and Vegfc (Fig. 6A). In support of this hypothesis, the formation of the intestinal vasculature is concomitant with the development of the intestine (Isogai et al., 2001;Okuda et al., 2012). However, we found that the expression levels of vegfc and vegfaa in intestinal epithelial cells remained unchanged following TNBS treatment; while this does not rule out a role for these cells in IIL, it led us to focus on the role of leukocytes in this process.
To determine the role of macrophages during IIL we utilised the nitroreductase-mediated cell ablation system (Pisharath and Parsons, 2009). Nitroreductase-mediated cell ablation has been successfully used in zebrafish to remove a variety of cell types including macrophage-lineage cells (Petrie et al., 2014). We found that IIL was suppressed when mpeg-1-expressing cells were ablated with metronidazole. In contrast, ablation of neutrophils had no effect on IIL, suggesting that macrophages are the main driver of inflammatory lymphangiogenesis in the intestine. Macrophages are thought to contribute to inflammatory lymphangiogenesis by two mechanisms; the first is via "transdifferentiation" into lymphatic endothelial cells (Kerjaschki et al., 2006;Maruyama et al., 2005) and the second is through the up regulation of prolymphatic growth factors such as VEGF-C, either directly via secretion or indirectly by the release of pro-inflammatory cytokines. (Baluk et al., 2005;Cursiefen et al., 2004;Harvey and Gordon, 2012;Kataru et al., 2009;Kim et al., 2009;Kubota et al., 2009;Maruyama et al., 2005;Tan et al., 2014;Zhang et al., 2007). We believe the second mechanism is the predominant one in our model as the lymphatic expansion mediated by macrophages was sensitive to VEGFR inhibitors. Therefore, we propose that the recruitment of pro-lymphatic macrophages to the intestine following colitogenic challenge, contributes to zebrafish IIL (Fig. 6B). In support, reduced macrophage recruitment to the intestine following 5-ASA treatment correlated with decreased zebrafish IIL.
We have previously shown that intestinal inflammation following colitogenic challenge requires the presence of gut microbiota, as treatment with mixtures of the broad-spectrum antibiotics penicillin/ streptomycin or ampicillin/kanamycin inhibited the recruitment of leukocytes to the intestine (Oehlers et al., 2011). Metronidazole is an antibiotic, particularly against anaerobic microbes and has been used to treat patients with Crohn's disease (Freeman et al., 1997;Prantera et al., 1996). We found that standard doses of between 5-10 mM of metronidazole that were previously used to conduct nitroreductase-mediated cell ablation in the zebrafish (Pisharath and Parsons, 2009) inhibited IIL in control, non-nitroreductase expressing animals (data not shown), likely due to the antibiotic activity of the pro-drug. However, a reduced metronidazole dose (2.5 mM) ensured that non-nitroreductase expressing animals displayed IIL while still allowing robust ablation of leukocytes using our treatment regimen.
Although macrophage and neutrophil numbers increased throughout the larvae following TNBS treatment (Oehlers et al., 2011), inflammatory lymphangiogenesis was only observed in the intestine. This may be because inflammation was more severe in the intestine than in other areas of the larvae in this model. In support, we previously demonstrated that expression of the inflammatory cytokine, interleukin 8, is specifically upregulated in the intestine of larvae exposed to TNBS (Oehlers et al., 2011). In addition, inflammatory angiogenesis was not observed in our model, despite an increase in vegfaa-expressing macrophages and neutrophils in the intestine. Intestinal blood vessels start developing at around 2.5 dpf (Isogai et al., 2001) while intestinal lymphatics start developing at around 4 dpf (Okuda et al., 2012). The lymphatic-specific phenotype may therefore be due to the intestinal lymphatic vessels developing later than the intestinal blood vasculature, making lymphatic vessels more susceptible to increased vegfaa/c/d expression.
In summary, we have established a novel inflammatory lymphangiogenesis model in zebrafish larvae. We have shown that zebrafish larval macrophages and neutrophils express vegfaa, vegfc, and vegfd and that macrophages contribute to inflammatory lymphangiogenesis in the zebrafish intestine. Our model provides a new platform to investigate the mechanisms of inflammation-driven lymphatic vessel growth.
FACS 3-7 dpf zebrafish larvae were dissociated as previously described (Covassin et al., 2006;Oehlers et al., 2011). Fluorescent cells were isolated based on forward and side scatter characteristics and fluorescence expression. FACS-assisted cellular isolation was carried out using a FACSAria II (Becton Dickinson, San Diego, CA, USA). FACS analysis experiments were carried out on a BD LSRII (Becton Dickinson, San Diego, CA, USA).

RNA extraction and RT-PCR
Total RNA from embryos and FACS sorted cells was extracted using Trizol reagent and Trizol LS reagent, respectively using the manufacturer's instructions (Life Technologies, Carlsbad, CA, USA). The cDNA was synthesised using High-Capacity cDNA Reverse Transcription Kit (Life Technologies, Carlsbad, CA, USA) using 1 μg of FACS sorted RNA or 2 μg of total RNA. Primer pairs used for RT-PCR are listed in supplementary material Table S1.

qPCR
The qPCR was performed using the Platinum SYBR Green qPCR SuperMix-UDG with ROX (Life Technologies, Carlsbad, CA, USA) in an ABI PRISM 7900 HT Fast sequence detection system (Life Technologies, Carlsbad, CA, USA) as previously described (Oehlers et al., 2011). Primer pairs are listed in supplementary material Table S1. All primer pairs spanned an intron to control for any contaminating genomic DNA.

Live imaging
Confocal images were taken as previously described ) using a Nikon D-Eclipse C1 confocal microscope using z stacks 5 μm apart. Epifluorescence images were taken as previously described (Oehlers et al., 2011) using Leica MZ16A fluorescence stereomicroscope with DFC490 camera. Images were processed using FiJi image processing software (Schindelin et al., 2012), Photoshop CS5 (Adobe, San Jose, CA, USA), and Volocity 5.4 image analysis software (Improvision/Perkin Elmer Life and Analytical Sciences, Shelton, CT, USA).

Quantification of thoracic duct and intestinal lymphatic sprouts
Thoracic duct formation at 5 dpf was scored as previously described (Astin et al., 2014). ILS development was quantified by taking lateral confocal images of the left intestinal region of lyve1:DsRed2;kdrl:EGFP larvae at 7 dpf and measuring the number and total length of ILSs anterior to the boundary of 7th and 8th somites using Volocity 5.4 image analysis software.

Quantification of neutrophil and macrophage number
Neutrophil and macrophage number was quantified through a fluorescent microscope in the left side of the zebrafish intestine at 7 dpf using lyz:EGFP and mpeg1:EGFP transgenics, respectively.

Nitroreductase-mediated cell ablation
For macrophage-lineage and neutrophil ablation, 2.3 dpf embryos were pretreated with 2.5 mM metronidazole (Sigma-Aldrich). At 3 dpf, embryos were co-treated with either TNBS+2.5 mM metronidazole or 2.5 mM metronidazole only and maintained until 7 dpf. Embryos were kept in the dark throughout.

Statistical analysis
All statistical analysis and graph plotting was done using GraphPad Prism version 5.0d (GraphPad Software). For single comparisons, unpaired t-test (normal distribution) or Mann-Whitney U-test were used. For multiple comparisons, one way ANOVA with Dunnett's test (multiple comparisons to control) or Turkey test (compare all data pairs) were used. P values of less than 0.05 were considered statistically significant.