Tyrosine motifs are required for prestin basolateral membrane targeting

ABSTRACT Prestin is targeted to the lateral wall of outer hair cells (OHCs) where its electromotility is critical for cochlear amplification. Using MDCK cells as a model system for polarized epithelial sorting, we demonstrate that prestin uses tyrosine residues, in a YXXΦ motif, to target the basolateral surface. Both Y520 and Y667 are important for basolateral targeting of prestin. Mutation of these residues to glutamine or alanine resulted in retention within the Golgi and delayed egress from the Golgi in Y667Q. Basolateral targeting is restored upon mutation to phenylalanine suggesting the importance of a phenol ring in the tyrosine side chain. We also demonstrate that prestin targeting to the basolateral surface is dependent on AP1B (μ1B), and that prestin uses transferrin containing early endosomes in its passage from the Golgi to the basolateral plasma membrane. The presence of AP1B (μ1B) in OHCs, and parallels between prestin targeting to the basolateral surface of OHCs and polarized epithelial cells suggest that outer hair cells resemble polarized epithelia rather than neurons in this important phenotypic measure.


INTRODUCTION
The cochlear amplifier is responsible for the exquisite sensitivity of mammalian hearing (Davis, 1983). There is considerable experimental data implicating electromotility of outer hair cells as integral to this process (Ashmore, 1987;Brownell et al., 1985;Dallos and Evans, 1995;Geisler, 1993;Geisler and Sang, 1995;Russell and Nilsen, 1997;Santos-Sacchi, 2003). Electromotility in outer hair cells is brought about by prestin, a transmembrane protein of the SLC26 family (Zheng et al., 2000), and molecular evidence has now confirmed its importance to cochlear amplification (Gao et al., 2007;Liberman et al., 2002;Mellado Lagarde et al., 2008). The localization of prestin along the lateral wall of these elongated cylindrical cells is critical to electromotility (Dallos et al., 1991;Hallworth et al., 1993;Huang and Santos-Sacchi, 1993;Kalinec et al., 1992;Yu et al., 2006;Zheng et al., 2000). The presence of prestin along the lateral wall of the cell brings about the voltage mediated elongation and shortening of outer hair cells along its longitudinal axis. How prestin is targeted to the lateral wall of the cell has been indeterminate.
Hair cells are specialized epithelial cells that show features of both epithelial cells as well as neurons. Individual cells form apically located tight junctions with other cells and have apically located stereocilia that are analogous to apically located microvilli (Leonova and Raphael, 1997;Mahendrasingam et al., 1997). Hair cells resemble neurons in containing unstable membrane potentials that result from a plethora of voltage and mechanically sensitive ion channels. These channels are sharply segregated in the cell with mechanically sensitive channels located in stereocilia (Fettiplace, 2009). In contrast many of its voltage and ligand gated ion channels are located at the basolateral surface of the cell (Housley et al., 2006). Inner hair cells, in addition, have synaptic apparatus that is located at it basal pole (Glowatzki et al., 2008).
A large body of work in polarized epithelial cells has shown the segregation of proteins to the basolateral and apical ends of the cell that results in a segregation of function (Farr et al., 2009;Rodriguez-Boulan et al., 2005). This segregation of proteins occurs by sorting of proteins after exit from the Golgi. Since neurons demonstrate a similar segregation of function, it has been proposed that dendritic and axonal compartments are analogous to the basolateral and apical surface respectively of polarized epithelial cells (Bradke and Dotti, 1998;Dotti et al., 1991;Dotti and Simons, 1990;Pietrini et al., 1994). Hair cells, however, pose a dilemma since they have features of both epithelial cells and neurons. Critically, the dendritic and axonal ends of a hair cell are at opposite ends to the expected basolateral and apical ends of the cell. Thus mechanosensitive channels that serve as its receptors are present in stereocilia and not at the basolateral surface as would be expected by its dendritic extrapolation. Similarly, the synaptic apparatus of inner hair cells is located at the basal pole and not at the stereociliary apical end as would be expected by its axonal extrapolation.
A collation of previous experimental data would suggest that protein sorting in hair cells resembles that of polarized epithelial cells rather than neurons. Thus, prior work has shown the basolateral localization of a number of proteins that are classically sorted to the basolateral surface of polarized epithelial cells. These proteins include E-cadherin, b-catenin and Na/K ATPase in mammalian, chicken and zebrafish hair cells (Bian et al., 2011;Clemens Grisham et al., 2013;Leonova and Raphael, 1997;Mahendrasingam et al., 1997). Moreover, AP1B (m1B), a protein subunit in the AP1B clathrin protein complex integral to the basolateral sorting apparatus, is present in hair cells and its loss is important for the sorting of Na/K ATPase to the basolateral surface of hair cells (Clemens Grisham et al., 2013). This protein subunit, which was identified as critical to hair cell function in a forward screening of hair cell dysfunction mutants in zebrafish, is normally present only in epithelial cells and not in other cell types including neurons .
In this paper we demonstrate that prestin, the protein responsible for outer hair cell electromotility, is localized along the basolateral surface of polarized epithelial cells using a tyrosine signal motif that is dependent on the AP1B (m1B) subunit. These data further support an emerging concept that in hair cells protein sorting resembles that of epithelial cells rather than neurons. Previous work in other polarized CL4 epithelial cells have shown a similar sorting of two proteins, Espin and myosin Xa, associated with the stereocilliary apparatus to the apical surface of the cell and prestin to the basolateral surface of the cell. The implications to this concept are significant.

MATERIALS AND METHODS cDNA constructs and generation of mutants
Single or multiple amino acid substitutions were generated using QuickChange II or QuickChange II Multi site-directed mutagenesis kits (Stratagene, La Jolla, CA) with a gerbil prestin-YFP in pEYFPN1 vector (Clontech, Mountain View, CA) as a template. All mutations were confirmed by DNA sequencing, including the entire coding region.

Cochlea processing
Tissue from 3+ week old mice (C57BL/6) were obtained after animals were euthanized using CO 2 asphyxiation in accordance with Yale University IACUC protocol. The temporal bones were isolated and placed in 4% formaldehyde overnight after opening the round and oval windows. Cochlea were removed after micro-dissection of the temporal bones washed in incubation buffer three times and processed for immunostaining as described above.

Cell transfection and imaging
MDCK and LLC-PK cells were a gift from Dr Michael Caplans lab, Yale University (in turn obtained from ATCC) and cells were not subject to STR profiling. MDCK and LLC-PK cells plated on glass coverslips (or where applicable HEK cells and CHO cells) were transiently transfected with constructs using Fugene6 according to the manufacturer's instructions (Promega, Madison, WI). Cells were plated at 100% confluency to ensure polarization, and transfected 36 hours after plating. Cells were fixed at 30 hours after transfection unless otherwise indicated. Cells were imaged by confocal microscopy using a Zeiss LSM 510/510 meta after fixation as previously described . Image parameters (scan time per pixel, pixel density, z step) were kept constant in a given experimental paradigm to allow reliable comparison.
In siRNA experiments, MDCK cells were electroporated with Prestin YFP and siRNA to the medium subunit of AP1B (m1B) as previously described . We used a previously proven siRNA to m1B siRNA (m1B-M16 sequence 59-AACAAGCTGGTGACTGGCAAA-39 ] and a control chicken b-4 siRNA (chick KCNMB4, ) that were custom synthesized (Dharmacon, Lafayette, CO). Briefly, m1B siRNA (or chick KCNMB4 siRNA in control experiments) at 5 nM and PrestinYFP plasmid at 10 nM was resuspended with 1 million trypsinized MDCK cells in 100 ml of DMEM. Cells were electroporated using a square wave pulse with the following parameters: 300 V for 100 ms61 and 25 V for 20 ms611 with a 100 ms pause in between. The entire electroporation protocol was repeated once. Cells were incubated on ice for 30 minutes and plated in prewarmed complete medium in 24 well plates on glass coverslips at a density of 350,000 cells per well (100% confluency). The medium was changed daily and cells were fixed and imaged 72 hours later.
Sixteen bit Images were acquired on a Zeiss 510 laser scanning microscope using a 636water immersion lens (N.A 1.4), with fixed laser settings, a scan rate of 6.4 ms per pixel, a pinhole aperture of 1.0 Airy units, and fixed detector gain. Regions of interest were identified and fluorescence data extracted. We established that the fluorescence intensity was within the linear range and used mean fluorescence density as a measure of protein concentration.

Gated STED
Gated STED, a new method of super resolution confocal microsopy, was used to visualize prestin expression in OHCs. Mouse cochlea were dissected and incubated with anti-prestin antibody (1:100 goat antiprestin, Santa Cruz, CA) in incubation buffer overnight at 4˚C. Tissue was washed three times with incubation buffer and then incubated with donkey anti-goat Oregon Green 488 (1:100, Beckton Dickinson) at room temperature for 1 hour. Cells were washed three times in incubation buffer followed by two washes in PBS. Tissue was mounted in Prolong Gold (Invitrogen) and viewed using a Leica TCS SP8 Gated STED microscope (Vicidomini et al., 2011).

Image processing and data analysis
Images were processed after acquisition using Volocity software (Perkin Elmer, CA). Pearson's correlation was calculated after automated thresholding using the method of Costes et al. (Costes et al., 2004). All results are given as mean 6 s.e.m. Where appropriate, ANOVA with Bonferroni Multiple Comparisons test was used to test for significance in differences (Instat3, CA).

Electrophysiological recording
For electrophysiological recordings 100,000 Chinese hamster ovary (CHO) cells were transfected in 24-well plates using Lipofectamine (Invitrogen, Carlsbad, CA) as previously described .
Capacitance was evaluated using a continuous high-resolution 2-sine wave. Capacitance data were fitted to the first derivative of a two-state Boltzmann function: Q max is the maximum nonlinear charge transfer, V h the voltage at peak capacitance or half-maximal nonlinear charge transfer, V m the membrane potential, C lin linear capacitance, z the valence (a metric of voltage sensitivity), e the electron charge, k the Boltzmann's constant and T the absolute temperature. Q max is reported as Q sp the specific charge density, i.e. total charge moved normalized to linear capacitance. A students t-test was used to evaluate the effects of mutants on the different parameters of NLC.

RESULTS
OHCs express prestin and other basolateral markers along baso-lateral wall, and express the AP1B (m1B) subunit Previous theoretical treatments confirmed by electrophysiological and immunolocalization experiments have demonstrated the localization of prestin along the lateral wall of OHCs. We sought to further confirm these data using high resolution confocal microscopy. Adult mice cochlea were stained with antiprestin antibodies and visualized using gated STED microscopy. As shown in Fig. 1 prestin is sharply localized along the lateral wall of the OHCs. The protein staining was uniform through the lateral wall. At the basolateral pole of the cell however, there was a patchy clustering of prestin. In contrast, there was a gradual tapering of prestin staining at the apical end of the cell.
The localization of prestin to the basolateral surface of outer hair cells led us to reason that outer hair cells are analogous to polarized epithelial cells with anatomical and functional segregation of the cell into an apical and basolateral compartments. The apical segregation of the stereociliary apparatus and the basolateral segregation of the cochlear amplifier are consistent with this separation. Prior work in hair cells of different species has shown the presence of b-catenin and Na/K ATPase, two proteins identified as classically localized in the basolateral compartment of polarized epithelial cells, to be present in the basolateral surface of hair cells. As shown in Fig. 1 these two proteins -b-catenin and Na/K ATPase -were found to segregate along the basolateral surface of outer hair cells (Fig. 1), further confirming similarities between outer hair cells and polarized epithelial cells. We also determine that OHCs express the AP1B (m1b) subunit that has been shown to be important for basolateral sorting of proteins in polarized epithelial cells (Fig. 1).
Prestin has several tyrosine residues within a basolateral targeting sequence of which Y520 and Y667 are important for basolateral sorting In order to establish an experimental model system to tease apart the mechanisms underlying prestins basolateral targeting, we expressed it in MDCK cells. These cells have a long history of being used as a model system for studying polarized sorting in epithelial cells. As shown in Fig. 2 prestin tagged with YFP at its C-terminus is targeted to the basolateral surface of MDCK cells. The protein shows a similar pattern of distribution to b-catenin and Na/K ATPase, both well known basolaterally targeted proteins. Prestin lacking a YFP tag showed similar targeting to the basolateral membrane (data not shown).
A number of sequence motifs in a protein are important for the targeting of a protein to the basolateral surface of polarized epithelial cells. Prestin has several tyrosine residues contained within a YXXW motif in its C-terminus, and lacks other targeting motifs classically associated with basolateral targeting (including dileucine motifs, NPxY) (Brewer and Roth, 1991;Cancino et al., 2007;Gonzalez and Rodriguez-Boulan, 2009;Gravotta et al., 2007;Hunziker et al., 1991;Lin et al., 1997;Matter et al., 1992;Rodriguez-Boulan and Gonzalez, 1999;Weisz and Rodriguez-Boulan, 2009). These include the tyrosine residues Y520, Y526, Y616 and Y667 (shown in cartoon form in the two alternative membrane spanning models of prestin in supplementary material Fig. S1). In order to ascertain the importance of these residues for basolateral targeting, we individually mutated these residues and determined their basolateral localization. As shown in Fig. 2 mutation of Y520Q, and to a lesser extent Y667Q, resulted in a reduced expression of prestin on the basolateral surface of the cell. There was both increased intracellular retention (Fig. 2) of the protein as well as targeting to the apical surface of the cell (Fig. 3).
In order to further confirm that the effects of these mutations were specific in bringing about deficient basolateral targeting we sought to determine the effects of these mutations on the surface expression of prestin in non-polarized HEK and CHO cells. As shown in Fig. 4 wt type prestin and prestin with mutations at Y520 and Y667 were both targeted to the cell surface. However, both these mutations affected the function of the molecule. In CHO cells expressing these mutant proteins, an increase in non-linear capacitance, the widely accepted functional surrogate for electromotility, was absent with Y520Q and markedly diminished with Y667Q. Supplementary material Table S1 shows the parameters for NLC in these mutants.
The phenol ring within the tyrosine residues (Y520 and Y667) are important for basolateral targeting We sought to determine the importance of the stereo chemical properties of the tyrosine residue at position 520 and 667 to ascertain its effects on targeting to the basolateral surface of MDCK cells. We mutated individual tyrosine residues to serine or phenylalanine to mimic the effects of the side chain hydroxyl group and phenol ring respectively. We determined that substitution of tyrosine with phenylalanine at Y520 resulted in near complete recovery in basolateral targeting (Fig. 5). In contrast, mutation of Y520 to a serine residue (to mimic the side chain OH group) resulted in poor delivery of the protein to the basolateral surface (Fig. 5). Similarly, it is unlikely that the effects of these other mutations are mediated by their size or hydrophilicity since mutation of Y520 to alanine failed to restore . The differences between wt and Y520Q and Y667Q were considered significant. A one way ANOVA (parametric) with Bonferroni post test comparison yielded a p value of ,0.001 for wt vs Y520Q and wt vs Y667Q; wt vs Y616Q and Y667Q were not significant. The mean surface to total ratios were: wt 0.67 (+/20.016 SE, n522); Y520Q 20.39 (+/20.0129 SE, n520); Y526Q 0.78 (+/20.013 SE, n513); Y616Q 0.73 (+/20.02 SE, n513); Y667Q 0.40 (+/2.044 SE, n513). Here too the differences between wt and Y520Q and Y667Q were significant. A one-way ANOVA (parametric) with Bonferroni post-test comparison yielded a p value of ,0.001 for wt vs Y520Q and wt vs Y667Q. The scale bar is 10 microns. basolateral targeting of prestin (Fig. 5). Similar effects were seen with mutation of Y667.

Mutation of Y520 and Y667 residues results in retention within the Golgi
Previous experiments in other proteins targeted to the basolateral surface have shown that sorting of proteins is determined at the Golgi. We hypothesized that mutation of tyrosine residues at Y520 and Y667 would result in retention of the protein within the Golgi (and could explain at least partially, the increased amounts of the mutated protein retained within the cell). MDCK cells transfected with prestin YFP, Y520Q, or Y667Q were fixed at different times after transfection and retention within the Golgi determined by colocalization with the Golgi specific protein Giantin (Fig. 6) (Deborde et al., 2008). We determined that mutation of Y520Q resulted in a persistent retention of prestin within the Golgi. In contrast, Y667Q showed retention of prestin within the Golgi, which improved with time. Thus, Y667Q was localized within the Golgi at 12, 20 and 30 hours after transfection but demonstrated increasing efflux from the Golgi at 40 hours. In contrast, wt prestin had made a near complete exit from the Golgi by 20 hours. Y520Q showed little egress from the Golgi with continued retention within that organelle even at 40 hours.
Prestin exit from the Golgi to the plasma membrane is mediated by early recycling endosomes We then sought to determine the identity of organelles responsible for movement of prestin from the Golgi to the plasma membrane. For these experiments we transfected prestin YFP into MDCK cells. Transfected cells were kept at 19˚C to allow accumulation of protein within the Golgi (Golgi block) (Bian et al., 2013). Cells were incubated with transferrin Alexa 647 applied to cells for 10 minutes to label early/recycling endosomes. The cells were then immersed in fresh culture medium and fixed at different time points after raising the temperature to 37˚C to release proteins from Golgi block. As shown in Fig. 7 there was co-localization of vesicles containing Fig. 3. Mutation of Y520 and Y667 result in increased delivery of prestin to the apical surface of MDCK cells. MDCK cells transiently transfected with wt prestin YFP and the two constructs Y520Q prestin YFP, and Y667Q prestin YFP were fixed at 36 hours and stained with antibody to the apical marker GP130 (podohexin). There is significant apical targeting of Y520Q and Y667Q evident in the serial X-Y sections along the z axis and the corresponding X-Z sections at the bottom. The bottom panel shows Pearson's correlation of prestin YFP and GP130 confirming absent apical targeting of the wild type construct and apical targeting of Y520Q and Y667Q. The mean Pearson's correlation values were: wt 20.027 (+/20.011 SE, n57); Y520Q 0.105 (+/20.035 SE, n55); Y667Q 0.17 (+/20.063 SE, n511). The differences between wt prestin and Y520Q and wt prestin and Y667Q were significant. A one-way ANOVA (parametric) yielded a p value of ,0.01 between wt prestin and Y520Q, and a p value of ,0.001 between wt prestin and Y667Q. The scale bar is 5 microns. prestin YFP that were fixed 48 hours after transfection. Cells were stained with antibodies to Na/K ATPase and visualized by confocal microscopy. Wild type prestin YFP, Y520Q prestin YFP and Y667Q prestin YFP all target the plasma membrane as evidenced by its co-localization with plasma membrane Na/K ATPase. The lower panel shows NLC traces of different mutations at Y520 and Y667. The actual values of NLC parameters along with cell numbers are given in supplementary material Table S1. The scale bar is 10 microns.
both prestin YFP and transferrin Alexa 647. We interpret this data to suggest that prestin YFP use early/recycling endosomes to reach the basolateral surface of the cell.
Prestin targeting to the basolateral surface also involves the AP1B (m1B) pathway In other systems the use of tyrosine motifs for basolateral sorting in polarized epithelial cells has been shown to involve the AP1B (m1B) pathway. We sought to ascertain if AP1B (m1B) was important for sorting prestin to the basolateral surface of polarized epithelial cells. In initial experiments we used LLC-PK cells, which lack the AP1B (m1B) subunit. In these cells Prestin YFP shows decreased targeting to the basolateral surface of the cell (supplementary material Fig. S2). We then used siRNA knockdown of AP1B (m1B) to determine targeting of prestin YFP in MDCK cells. In these cells knock down treatment with siRNA to AP1B (m1B) resulted in apical targeting of the protein and reduced expression of the protein on the basolateral surface of the cell (Fig. 8).

DISCUSSION
In this paper we show for the first time that prestin is sorted to the basolateral surface of polarized epithelial (MDCK) cells using a tyrosine motif. We identified two tyrosine residues contained in the cassette YXXW motif that are likely important for its localization to the basolateral surface of the cell. Mutation of one of these residues Y520 resulted in apical targeting and intracellular retention of the protein. A second mutation of Y667 resulted in delayed exit from the Golgi to the basolateral surface with initial targeting to the apical surface of the cell and intracellular retention. With increasing time, however, there was progressive localization of this mutant at the basolateral surface of the cell. We believe mutation of these residues resulted in specific loss of basolateral targeting since the mutant protein was targeted to the apical surface of MDCK cells and since the protein showed plasma membrane targeting in non-polarized CHO and HEK cells. Nevertheless, it still remains a possibility that the loss of basolateral targeting resulted from a subtle misfolding of the protein that also affected its normal function. We were unable to demonstrate normal function as measured by the presence of nonlinear capacitance in these mutants expressed in CHO cells (Y520Q showed absent NLC while Y667Q showed diminished NLC). In this context, it should be noted that several mutations that also affected prestin function by subtle alterations in its folding, show normal basolateral targeting in MDCK cells (data not shown). We would also like to note previous experiments by Zheng et al. (Zheng et al., 2005), in which mutation of Y520 and Y526 together failed to show targeting to the surface of TSA and OK cells. We do not have an explanation for the discordance in our data although the experiments are not strictly comparable since Zheng et al. (Zheng et al., 2005) were describing the targeting of a double mutant (Y520A and Y526A) while we describe the targeting of single mutations (Y520 or Y667).
Our interpretation that prestin is sorted to the basolateral surface of cells using a tyrosine motif is further substantiated by two other related findings. First, we demonstrate that AP1B (m1B) is important for basolateral targeting, since expression of prestin in LLC-PK cells that lack this subunit resulted in decreased basolateral targeting. Moreover knockdown of AP1B (m1B) in MDCK cells also resulted in increased apical targeting of prestin with deficient basolateral targeting of the protein. It is now believed that the tyrosine residues are critical for direct interaction with AP1B (m1B) and bringing about basolateral targeting of proteins (Bonifacino and Dell'Angelica, 1999;Carvajal-Gonzalez et al., 2012;Fields et al., 2007). We also demonstrate using Golgi block experiments that prestin uses recycling endosomes as a post Golgi transport intermediate to transit from the Golgi to the basolateral plasma membrane. The use of AP1B (m1B) has been demonstrated by several other proteins using tyrosine residues contained in a YXXW motif to  /20.045 SE,n56). A one way ANOVA revealed significant differences between wt prestin and Y520A (P,0.01), wt prestin and Y520Q (P,0.001), and wt prestin and Y520S (P,0.001). The differences between wt prestin and Y520F were not significant. The scale bar is 10 microns.
target the basolateral membrane (Adair-Kirk et al., 2003;Ang et al., 2004;Duffield et al., 2004;Fields et al., 2007;Fölsch et al., 1999;Fölsch et al., 2003;Gravotta et al., 2007;Sugimoto et al., 2002). Moreover, the use of transferrin containing endosomes has been shown with basolateral proteins that use a tyrosine motif to exit the Golgi (Ang et al., 2004;Donoso et al., 2009). In prior experiments membrane targeting to the surface of CL4 cells, which are derived from LLC-PK1 cells have been observed (Zheng et al., 2010). In the absence of data on the expression of AP1B (m1B) in CL4 cells used for these experiments or the relative surface expression of prestin in these cells, it is difficult to comment on the significance of these data (Zheng et al., 2010).
The implications to our data are greatest to hair cells. Our data further suggest that hair cells are similar to polarized epithelial cells with differential sorting of proteins to the apical Fig. 6. Egress from the Golgi is poor/absent in Y520Q and delayed in Y667Q. MDCK cells transiently transfected with prestin YFP, and the mutations Y520Q and Y667Q contained in the prestin YFP cassette were fixed after 12, 20, 30 and 40 hours after transfection. The cells were then stained with antibodies to the Golgi protein Giantin. While prestin YFP exits the Golgi at 20 hours, Y520 dos not egress from the Golgi and Y667Q leaves the Golgi after 40 hours. A graphical form of Pearson's correlation between prestin YFP and Giantin at the different time points is shown below. For wt prestin there was a significant difference on one way ANOVA between the Pearson's correlation between prestin and Giantin at all times compared to 12 hours: 12 hours vs 20 hours, P,0.05; 12 hours vs 30 hours, P,0.001; and 12 hours vs 40 hours P,0.001. In contrast, the differences in Pearson's correlation between Y520Q and Giantin at all times compared to 12 hours were not significantly different (P.0.05 on one way ANOVA). For Y667Q the Pearson's correlation with Giantin was significantly different on one way ANOVA between 12 hours and 40 hours (P,0.05), while the remaining two time points were not significantly different (12 hours vs 20 hours, P.0.05; 12 hours vs 30 hours, P.0.05). and basolateral surface of the cell. Hair cells could be thought of as having different compartments -an apical surface important for transducing sound and a basal pole that is important for synaptic transmission in inner hair cells and a basolateral pole that is important for housing the cochlear amplifier and its processes. Our data suggest that integral to this segregation of function is the segregation of proteins to different apical and basal compartments of the cell along the lines demonstrated in polarized epithelial cells. Other work has also shown the apical sorting in CL4 cells of proteins identified as important for stereociliary and transduction function in hair cells (Zheng et al., 2010). At least in the context of protein sorting, hair cells seem to have retained features of polarized epithelial cells rather than neurons. The expected segregation of proteins along a dendritic and axonal separation would result in a reversal of the observed localization of proteins in hair cells. Perhaps one explanation for this unexpected finding is that hair cells resemble epithelial cells more than they do neurons. Importantly, hair cells are derived from the embryonic otic placode, a thickened segment of the cranial ectoderm that invaginates separately from the neural tube to form the otocyst. Consistent with its epithelial phenotype, hair cells express the clathrin adaptor protein AP1B (m1B) subunit that is present only in polarized epithelial cells and lacking in neurons. Significantly, this subunit is important for the localization of several proteins that, like prestin, use a tyrosine motif to target to the basolateral surface of the cell. It remains to be established if prestin uses tyrosine residues and /or the AP1B (m1B) subunit to target to the lateral wall of outer hair cells. Fig. 7. Prestin YFP uses transferrin containing endosomes to transit from the Golgi to the basolateral membrane. MDCK cells were transiently transfected with prestin-YFP and kept at 19˚C to induce Golgi block. Cells were incubated with transferrin -Alexa 647 for 10 minutes. The incubation temperature was raised to 37˚C after which cells were fixed at 0, 1 minute, 5 minutes, 10 minutes and 15 minutes. Cells were then imaged with confocal microscopy. Shown are confocal images in the X-Y plane at the basolateral Z axis of the cell that were fixed at different times after raising the temperature to 37˚C. With increasing time there is co-localization of prestin-YFP exiting the Golgi with transferring -Alexa 647. The right hand panels shows merged and individual Alexa 647 and prestin-YFP images of an enlarged area at 15 minutes after raising the temperature to 37˚C. Colocalization of prestin YFP and transferrin 647 is demonstrated. The scale bar is 10 microns. Fig. 8. Targeting of prestin YFP to the basolateral membrane requires AP1B (m1B). MDCK cells were electroporated with prestin YFP plasmid and siRNA to AP1B (m1B). Cells were plated at confluent density and fixed after 30 hours. Cells were stained with antibodies to the apical marker GP130 and visualized by confocal microscopy. Shown are X-Y images along the z axis of transfected cells. The lowest panel shows the corresponding X-Z sections. Transfection of MDCK cells with siRNA to AP1B (m1B) resulted in an apical localization of prestin YFP and near absence in targeting to the basolateral surface. In contrast, cells co-transfected with control siRNA (chicken KCNMB4) resulted in basolateral targeting of prestin YFP. The scale bar is 10 microns.