A symbiotic alga was successfully isolated from the soil moss Entodon obtusatus from the Guandi Mountains, Shanxi Province, China, and cultivated under axenic conditions. Morphological observations showed that the symbiotic alga was similar to Chlorococcum. Based on phylogenetic analysis of 18S rRNA and rbcL genes and ITS regions, Chlorococcum sp. GD was identified as C. sphacosum. Three data sets were congruent for those aspects of the respective topologies that were relatively robust, and differed for those parts of the topologies that were not. This strain was cultured in BG11 medium to test its growth and biodiesel properties. It produced a lipid content of nearly 40%, and achieved biomass concentration of 410 mg L−1 and lipid productivity of 6.76 mg L−1d−1, with favorable C16:0 (23.10%) and C18:1 (21.62%) fatty acid content. This alga appears to have potential for use in biodiesel production.
- Received July 6, 2016.
- Accepted August 15, 2016.
- © 2016. Published by The Company of Biologists Ltd
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