RT Journal Article
SR Electronic
T1 Phase-contrast X-ray microtomography of mouse fetus
JF Biology Open
JO Biology Open
FD Company of Biologists
SP BIO2012430
DO 10.1242/bio.2012430
A1 Hoshino, Masato
A1 Uesugi, Kentaro
A1 Yagi, Naoto
YR 2012
UL http://bio.biologists.org/content/early/2012/02/09/bio.2012430.abstract
AB A phase-contrast X-ray microtomography system using the Talbot imaging has been built at the SPring-8 synchrotron radiation facility. This system has much higher density resolution than absorption-based X-ray microtomography. The tomographic sections of formalin-fixed mouse fetuses obtained with this method clearly depict various organs without any staining at a pixel resolution of up to 5 µm. Since this technique allows us to obtain three-dimensional structural information without sectioning, it will be particularly useful to examine anomalies that take place during development. It can be also used to quantitatively measure volume and mass of organs during development.