PT - JOURNAL ARTICLE AU - Lagendijk, Anne Karine AU - Moulton, Jon D. AU - Bakkers, Jeroen TI - Revealing details: whole mount microRNA <em>in situ</em> hybridization protocol for zebrafish embryos and adult tissues DP - 2012 Apr 11 TA - Biology Open 4099 - http://bio.biologists.org/content/early/2012/04/11/bio.2012810.short 4100 - http://bio.biologists.org/content/early/2012/04/11/bio.2012810.full AB - Non-coding microRNA (miRNA) molecules bind their target mRNAs and thereby modulate the amount of protein produced. To understand the significance of a potential miRNA-mRNA interaction, temporal and spatial information on miRNA and mRNA expression is essential. Here, we provide a detailed protocol for miRNA whole mount in situ hybridization. We introduce the use of Morpholino based oligos as antisense probes for miRNA detection, in addition to the current “gold standard” locked nucleic acid (LNA) probes. Furthermore we have modified existing miRNA in situ protocols thereby improving both sensitivity and resolution of miRNA visualization in whole zebrafish embryos and adult tissues.