PT  - JOURNAL ARTICLE
AU  - Lagendijk, Anne Karine
AU  - Moulton, Jon D.
AU  - Bakkers, Jeroen
TI  - Revealing details: whole mount microRNA <em>in situ</em> hybridization protocol for zebrafish embryos and adult tissues
DP  - 2012 Apr 11
TA  - Biology Open
4099  - http://bio.biologists.org/content/early/2012/04/11/bio.2012810.short
4100  - http://bio.biologists.org/content/early/2012/04/11/bio.2012810.full
AB  - Non-coding microRNA (miRNA) molecules bind their target mRNAs and thereby modulate the amount of protein produced. To understand the significance of a potential miRNA-mRNA interaction, temporal and spatial information on miRNA and mRNA expression is essential. Here, we provide a detailed protocol for miRNA whole mount in situ hybridization. We introduce the use of Morpholino based oligos as antisense probes for miRNA detection, in addition to the current “gold standard” locked nucleic acid (LNA) probes. Furthermore we have modified existing miRNA in situ protocols thereby improving both sensitivity and resolution of miRNA visualization in whole zebrafish embryos and adult tissues.