- Reprogramming towards totipotency is greatly facilitated by synergistic effects of small molecules
Summary: The optimized culture condition with small molecules is sufficient to allow highly efficient mouse cloning by removing epigenetic barriers to reprogramming.
- Systematic evaluation of markers used for the identification of human induced pluripotent stem cells
Summary: The use of hESC-like morphology, retroviral transgene silencing and temporal expression of pluripotency markers are compared as methods to aid in the identification of hiPSC clones.
- Co-expression networks in generation of induced pluripotent stem cells
Summary: We developed a novel adenoviral iPSC reprogramming vector integrating Yamanaka's four factors in a single cassette, allowing for the identification of biologically relevant co-expression networks.
- Generation of primitive neural stem cells from human fibroblasts using a defined set of factors
Summary: A novel method for the generation of self-renewable, multipotent and neural lineage-restricted LIF-dependent induced primitive neural stem cells (LD-iNSCs) from human fibroblasts with the potential to facilitate human neuronal studies.