- Disruption of pdgfra alters endocardial and myocardial fusion during zebrafish cardiac assembly
Summary: The molecular mechanisms regulating cardiac fusion are not well understood. Here, we show that platelet-derived growth factor receptor alpha is essential for normal endocardial and myocardial fusion during zebrafish development.
- Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model
Summary: Patient-derived primary cells maintain their original phenotype when cultured on 3D scaffolds, and their in vivo behavior can be effectively studied with innovative zebrafish models.
- Utilising polymorphisms to achieve allele-specific genome editing in zebrafish
Summary: Heterozygous single nucleotide polymorphisms in CRISPR/Cas9 target sites bias genome editing in favour of alleles with perfect complementarity to gRNAs, a feature which can be exploited for chromosome-specific editing.
- Molecular insights into Adgra2/Gpr124 and Reck intracellular trafficking
Summary: This work uncovers molecular determinants of Adgra2/Gpr124 and Reck trafficking to the plasma membrane where the partners meet to act as potent Wnt7-specific Wnt/β-catenin signaling co-activators required for brain vascularization.
- Heterotypic interactions regulate cell shape and density during color pattern formation in zebrafish
Summary: The conspicuous pigmentation pattern of zebrafish is produced by three kinds of interacting pigment cells. Here we address the cellular consequences of these interactions in wild-type fish and mutants with altered pigment patterns.
- αE-catenin-dependent mechanotransduction is essential for proper convergent extension in zebrafish
Summary: Cadherin adhesions transduce tension across the junction-actomyosin axis into proportional biochemical responses via α-catenin. Here we show for the first time how this function of the cadherin complex is important during zebrafish morphogenesis.
- Zebrafish P54 RNA helicases are cytoplasmic granule residents that are required for development and stress resilience
Summary: Stress granules are formed by mRNAs stalled in translation during stress conditions. P54 RNA helicases from zebrafish reside in cytoplasmic granules and are essential for heat-shock resilience.
- Alcohol exposure leads to unrecoverable cardiovascular defects along with edema and motor function changes in developing zebrafish larvae
Summary: Alcohol exposure causes cardiovascular defects along with edema and motor function changes in zebrafish larvae that are unrecoverable in the post-exposure period.
- Cortisol-treated zebrafish embryos develop into pro-inflammatory adults with aberrant immune gene regulation
Summary: Chronically elevated glucocorticoid stress signaling during early zebrafish development has long-term consequences that affect immune gene expression and regulation in adulthood.
- Differences in cardiovascular toxicities associated with cigarette smoking and snuff use revealed using novel zebrafish models
Summary: We analyze common and distinct developmental and cardiovascular toxicities caused by cigarette smoke and snuff, and include a new zebrafish embryo model for studying abdominal aortic aneurysm.