BIO2012885 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Western blots following GRNA affinity chromatography to assay for the presence of Sqd, Syp and Tubulin as indicated. Lysate contains 2.5% of the total wild-type (OrR) ovarian lysate added to each GRNA matrix.

• Supplemental Figure S2 -

  Fig. S2. syp isoform-specific RT-PCR from a range of tissues and developmental stages. Two RNAi lines were tested, 33011 and 33012 (obtained from the VDRC), driven by the ubiquitous actinGal4 driver. RNAi knockdown with both lines is not as severe as that observed in the mutant and does not target isoform G, but is sufficient to result in pupal lethality.

• Supplemental Table S1 -

  Table S1. List of all proteins identified by GRNA chromatography using 5′ORF RNA. The 16 proteins highlighted in bold were found only to associate with 5′ORF RNA.

• Supplemental Table S2 -

  Table S2. Proteins identified by GRNA affinity chromatography. The proteins able to associate with 5′ORF are listed with information as to whether a protein or its orthologues (given in brackets) are known to be present in the transport RNPs of localized mRNAs. If it is known that the protein and the RNA can directly interact, this RNA is highlighted in bold.

• Supplemental Table S3 -

  Table S3. Antibody stocks and markers.

• Supplemental Table S4 -

  Table S4. List of primers used for cloning, and for RT and real time PCR analysis.