FUTURE LEADER REVIEW
- Cardiac myosin super relaxation (SRX): a perspective on fundamental biology, human disease and therapeutics
Summary: A discussion of the role and regulation of myosin super relaxation in biology, evolution, human disease, and cardiac therapeutics.
RESEARCH ARTICLES
- Stability of amino acids and related amines in human serum under different preprocessing and pre-storage conditions based on iTRAQ®-LC-MS/MS
Summary: We investigated the stability of amino acids in serum samples that underwent prolonged storage at 4°C and 22°C, and repeated freeze-thaw cycles at −80°C using stable isotope iTRAQ labeling and liquid chromatography tandem mass spectrometry.
- Segregation of brain and organizer precursors is differentially regulated by Nodal signaling at blastula stage
Summary: Nodal signaling is involved in the delimitation of the blastula cell populations that give rise to the brain and axial mesoderm in Xenopus.
- Tetraspanin18 regulates angiogenesis through VEGFR2 and Notch pathways
Summary: Tspan18 is a transmembrane protein with highly restricted expression in endothelial cells and a critical regulator of VEGF and Notch pathways. It regulates artery–vein specification, vessel patterning and vessel stability.
- Unique histological features of the tail skin of cotton rat (Sigmodon hispidus) related to caudal autotomy
Summary: The unique histological structures in derimis, subcutis and coccygeal artery of the tail skin are related to the caudal autotomy mechanism in the cotton rat.
- Sex differences in postprandial blood glucose and body surface temperature are contingent on flight in the fruit bat, Cynopterus sphinx
Summary: The level of sex differences in metabolic traits is affected by flight in Cynopterus sphinx, a finding that has general implications for sex-specific life-history evolution in fruit bats.
- Smoking flies: testing the effect of tobacco cigarettes on heart function of Drosophila melanogaster
Summary: Nicotine, a substance present in tobacco, resembles insecticide compounds such as neonicotinoids, and affects the heart function in Drosophila melanogaster.
- Single-cell sequencing reveals the potential oncogenic expression atlas of human iPSC-derived cardiomyocytes
Summary: The single-cell expression atlas in the cardiomyocytes generated from human iPSCs provide potential carcinogenic information for the clinical application of human iPSC-derived cells.
- Quantified growth of the human embryonic heart
Summary: This paper provides quantitative data on the growth of 58 structures of the human embryonic heart between weeks 4 and 8 of development.
- Dissecting dynamics and differences of selective pressures in the evolution of human pigmentation
Summary: This study developed a novel approach to estimate selection strength over time and applied it on the evolution of human pigmentation.
- A method to culture human alveolar rhabdomyosarcoma cell lines as rhabdospheres demonstrates an enrichment in stemness and Notch signaling
Summary: Here we report on a method to culture human PAX3-FOXO1 fusion-positive rhabdomyosarcoma cells in three dimensions, and use these rhabdospheres as a novel tool to study their stemness and chemoresistance signaling mechanisms.
- Auxin confers protection against ER stress in Caenorhabditis elegans
Summary: Auxin, commonly used to induce conditional protein degradation, promotes ER stress resistance in C. elegans through the unfolded protein response (UPR).
- A modifier screen identifies regulators of cytoskeletal architecture as mediators of Shroom-dependent changes in tissue morphology
Summary: Using Drosophila as a model system, we identify F-actin and microtubules as important determinants of how cells and tissues respond to Shroom induced contractility.
METHODS & TECHNIQUES
- Three-dimensional model of glioblastoma by co-culturing tumor stem cells with human brain organoids
Summary: We describe a method to co-culture glioblastoma stem cells with human cerebral organoids in order to model tumor complexity and recapitulate cellular heterogeneity in vitro.
- Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku
Summary: The authors describe a fluorescently tagged Ku70 protein to monitor replication stress in live S. pombe cells.
- Design of a 3D printed, motorized, uniaxial cell stretcher for microscopic and biochemical analysis of mechanotransduction
Summary: Description and testing of a 3D printed uniaxial stretcher designed for the microscopic and biochemical analysis of mammalian cells subjected to mechanical stretch.