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Eye imaginal disc from a third instar Drosophila melanogaster larva stained to detect the actin associated proteins Shroom (red) and Enabled (cyan). Jeffrey D. Hildebrand and colleagues show that Enabled is widely expressed throughout the imaginal disc while Shroom shows more restricted expression in the posterior cells of the morphogenetic furrow, pre-ommatidia clusters, and newly formed ommatidia. Within those cells that express both proteins, Shroom and Enabled co-localize to restricted populations of cell-cell junctions. Anterior is to the left. Image licensed under a Creative Commons Attribution 4.0 International license.