Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Interviews
    • Sign up for alerts
  • About us
    • About BiO
    • Editors and Board
    • Editor biographies
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contact
    • Contact BiO
    • Advertising
    • Feedback
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in

Search

  • Advanced search
Biology Open
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Biology Open

Advanced search

RSS   Twitter   Facebook   YouTube

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Interviews
    • Sign up for alerts
  • About us
    • About BiO
    • Editors and Board
    • Editor biographies
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contact
    • Contact BiO
    • Advertising
    • Feedback
Research Article
Cenp-meta is required for sustained spindle checkpoint
Thomas Rubin, Roger E. Karess, Zohra Rahmani
Biology Open 2014 3: 522-528; doi: 10.1242/bio.20148490
Thomas Rubin
*Present address: Department of Genetics and Developmental Biology, Institut Curie, 75248 Paris Cedex 05, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Roger E. Karess
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zohra Rahmani
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: rahmani.zohra@ijm.univ-paris-diderot.fr
  • Article
  • Figures & tables
  • Supp info
  • Info & metrics
  • eLetters
  • PDF + SI
  • PDF
Loading

Data supplements

  • bio.20148490 Supplementary Material

    Files in this Data Supplement:

    • Supplementary Material - Thomas Rubin et al. doi: 10.1242/bio.20148490
    • Movie 1 - Movie 1. Mitotic timing in a WT neuroblast. WT Drosophila larval neuroblast labeled with RFP-Spc25 (red). Corresponds to Fig. 2B. Images were acquired by confocal spinning disk microscopy. Frames were taken every 20 seconds for 9.6 minutes. The movie is shown at 8 frames/s.
    • Movie 2 - Movie 2. Mitotic timing in a cenp-metaΔ mutant neuroblast. cenp-metaΔ mutant Drosophila larval neuroblast labeled with RFP-Spc25 (red). Corresponds to Fig. 2C. Images were acquired by confocal spinning disk microscopy. Frames were taken every 20 seconds for 13.6 minutes. The movie is shown at 8 frames/s.
    • Movie 3 - Movie 3. Mitotic timing in a cenp-metaΔ mad2P mutant neuroblast. cenp-metaΔ mad2P mutant Drosophila larval neuroblast labeled with RFP-Spc25 (red). Corresponds to Fig. 2D. Images were acquired by confocal spinning disk microscopy. Frames were taken every 20 seconds for 9.3 minutes. The movie is shown at 8 frames/s.
    • Movie 4 - Movie 4. Mad2 dynamics in a WT neuroblast. WT Drosophila larval neuroblast labeled with GFP-Mad2 (green). Corresponds to Fig. 3A. Images were acquired by confocal spinning disk microscopy. Frames were taken every 20 seconds for 16.3 minutes. The movie is shown at 8 frames/s.
    • Movie 5 - Movie 5. Mad2 dynamics in a cenp-metaΔ mutant neuroblast. cenp-metaΔ mutant Drosophila larval neuroblast labeled with GFP-Mad2 (green). Corresponds to Fig. 3B. Images were acquired by confocal spinning disk microscopy. Frames were taken every 20 seconds for 10.7 minutes. The movie is shown at 8 frames/s.
    • Movie 6 - Movie 6. Mad2 dynamics in a cenp-metaΔ mutant neuroblast. cenp-metaΔ mutant Drosophila larval neuroblast labeled with GFP-Mad2 (green). Corresponds to Fig. 3C. Images were acquired by confocal spinning disk microscopy. Frames were taken every 20 seconds for 14.7 minutes. The movie is shown at 8 frames/s.
    • Movie 7 - Movie 7. BubR1 dynamics in a WT neuroblast. WT Drosophila larval neuroblast labeled with GFP-rod (green; left) and RFP-BubR1 (red; middle). Merged images are shown in the right panel. Corresponds to Fig. 3D. Images were acquired by confocal spinning disk microscopy. Frames were taken every 20 seconds for 5 minutes. The movie is shown at 8 frames/s.
    • Movie 8 - Movie 8. BubR1 dynamics in a cenp-metaΔ mutant neuroblast. cenp-metaΔ mutant Drosophila larval neuroblast labeled with GFP-rod (green; left) and RFP-BubR1 (red; middle). Merged images are shown in the right panel. Corresponds to Fig. 3E. Images were acquired by confocal spinning disk microscopy. Frames were taken every 20 seconds for 8.3 minutes. The movie is shown at 8 frames/s.
    • Movie 9 - Movie 9. BubR1 dynamics in a cenp-metaΔ mutant neuroblast. cenp-metaΔ mutant Drosophila larval neuroblast labeled with GFP-rod (green; left) and RFP-BubR1 (red; middle). Merged images are shown in the right panel. Corresponds to Fig. 3F. Images were acquired by confocal spinning disk microscopy. Frames were taken every 20 seconds for 4.3 minutes. The movie is shown at 8 frames/s.
    • Movie 10 - Movie 10. Cyclin B degradation in a WT neuroblast. WT Drosophila larval neuroblast labeled with GFP-cyclin B (green; top left) and RFP-Rod (red; top right). Merged images are shown in the bottom left panel. Corresponds to Fig. 4A. Images were acquired by confocal spinning disk microscopy. Frames were taken every 20 seconds for 8.9 minutes. The movie is shown at 8 frames/s.
    • Movie 11 - Movie 11. Cyclin B degradation in a cenp-metaΔ mutant neuroblast. cenp-metaΔ mutant Drosophila larval neuroblast labeled with GFP-cyclin B (green; top left) and RFP-Rod (red; top right). Merged images are shown in the bottom left panel. Corresponds to Fig. 4B. Images were acquired by confocal spinning disk microscopy. Frames were taken every 20 seconds for 17.2 minutes. The movie is shown at 8 frames/s.
Previous ArticleNext Article
Back to top
Previous ArticleNext Article

This Issue

RSSRSS

Keywords

  • Mitosis
  • Spindle assembly checkpoint
  • Metaphase
  • Cenp-E
  • BUBR1
  • APC/C
  • Kinetochore

 Download PDF

Email

Thank you for your interest in spreading the word on Biology Open.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Cenp-meta is required for sustained spindle checkpoint
(Your Name) has sent you a message from Biology Open
(Your Name) thought you would like to see the Biology Open web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Research Article
Cenp-meta is required for sustained spindle checkpoint
Thomas Rubin, Roger E. Karess, Zohra Rahmani
Biology Open 2014 3: 522-528; doi: 10.1242/bio.20148490
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Research Article
Cenp-meta is required for sustained spindle checkpoint
Thomas Rubin, Roger E. Karess, Zohra Rahmani
Biology Open 2014 3: 522-528; doi: 10.1242/bio.20148490

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article Navigation

  • Top
  • Article
    • ABSTRACT
    • INTRODUCTION
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • Acknowledgements
    • Funding
    • Footnotes
    • References
  • Figures & tables
  • Supp info
  • Info & metrics
  • eLetters
  • PDF + SI
  • PDF

Related articles

Cited by...

More in this TOC section

  • The basal release of endothelium-derived catecholamines regulates the contractions of Chelonoidis carbonaria aorta caused by electrical-field stimulation
  • Smoking flies: Testing the effect of tobacco cigarettes on heart function of Drosophila melanogaster
  • Bisphenol A promotes stress granule assembly and modulates the integrated stress response
Show more RESEARCH ARTICLE

Similar articles

Other journals from The Company of Biologists

Development

Journal of Cell Science

Journal of Experimental Biology

Disease Models & Mechanisms

Advertisement

Biology Open and COVID-19

We are aware that the COVID-19 pandemic is having an unprecedented impact on researchers worldwide. The Editors of all The Company of Biologists’ journals have been considering ways in which we can alleviate concerns that members of our community may have around publishing activities during this time. Read about the actions we are taking at this time.

Please don’t hesitate to contact the Editorial Office if you have any questions or concerns.


2020 at The Company of Biologists

Despite 2020’s challenges, we achieved a lot at The Company of Biologists. In the midst of the pandemic, we have seen long-term projects and new ventures come to fruition. Read our full lowdown of 2020.


Interview- Sebastian Markert

Sebastian Markert is first author of a paper in BiO using C. elegans to model amyotrophic lateral sclerosis. In an interview, he talks about the potential implications of his work and his future plans.


Three communities to support biologists to everywhere

Online communities have never been more important. If you’re looking for somewhere to meet fellow scientists, take part in topical discussions and find virtual events in your field, take a look at each of our community sites:

  • The Node: the community site for and by developmental biologists
  • preLights: the preprint highlights service run by the biological community
  • FocalPlane: the community site for microscopists and biologists alike

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Interviews
  • Sign up for alerts

About us

  • About BiO
  • Editors and Board
  • Editor biographies
  • Grants and funding
  • Journal Meetings
  • Workshops
  • The Company of Biologists

For Authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Article types
  • Manuscript preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access

Journal Info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Sign up for alerts

Contact

  • Contact BiO
  • Advertising
  • Feedback

Twitter   YouTube   LinkedIn

© 2021   The Company of Biologists Ltd   Registered Charity 277992