Article Figures & Tables
Figures
- Fig. 1.
Mating of a marbled crayfish female with a P. fallax male. The male (top) holds the female firmly with the chelipeds and ischial hooks and his gonopods are plugged into the female's spermatheca.
- Table 1.
Crossbreeding experiments between marbled crayfish, P. fallax and P. alleni
- Table 2.
Parentage analysis in crossbreeds of marbled crayfish x P. fallax
- Fig. 2.
Comparison of complete mitochondrial genomes of marbled crayfish, P. fallax and P. alleni. The sequences of marbled crayfish from two laboratory populations (Heidelberg, Petshop) and two wild populations (Moosweiher, Madagascar) are completely identical. In contrast, the sequences of P. fallax and P. alleni differ in 144 and 1165 SNPs (vertical lines) from marbled crayfish, respectively. Purple bars indicate positions of 12S rRNA and cytochrome oxidase subunit I (COI) fragments that were used for an earlier phylogenetic analysis (Martin et al., 2010).
- Fig. 3.
Ploidy status of the marbled crayfish genome. (A) Microsatellite locus PclG-02 in marbled crayfish showing a combination of three alleles of 267 bp, 271 bp and 303 bp fragment length. (B) Flow cytometry of haemocytes of P. fallax (Pf) and marbled crayfish (mc) revealing an approximately 1.4 fold increased DNA content in marbled crayfish. The right panel shows the means±standard deviations (s.d.) of two biological and three technical replicates. Differences are highly significant (P=1.33×10−7, Welsh two-sided t-test).
- Fig. 4.
Differences in global DNA methylation between marbled crayfish (red) and P. fallax (blue). Three complete juveniles and major organs of three adult females were analysed in each crayfish. Note the consistently and significantly greater methylation levels in P. fallax (P=1.48×10−7 for the sum of all samples, Welsh two-sided t-test). Error bars represent s.d.
- Fig. 5.
Comparison of morphological characters between marbled crayfish and P. fallax. (A) Annulus ventralis from exuvia of marbled crayfish. (B) Annulus ventralis of P. fallax. (C) Annulus ventralis of P. alleni. Note striking structural difference to sperm receptacles of marbled crayfish and P. fallax. (D) Areola of marbled crayfish. (E) Areola of P. fallax. (F) Left cheliped of marbled crayfish of 8.4 cm TL. (G) Left cheliped of P. fallax female of 4.7 cm TL. Form, dentation and setation of the chelae are very similar in both species. (H) Coloration of cephalothorax in marbled crayfish. (I) Coloration of cephalothorax in P. fallax male (photo: C. Lukhaup).
- Fig. 6.
Comparison of growth between marbled crayfish and P. fallax. The three groups were reared for 250 days at 20°C under identical conditions and fed with the same food ad libitum. The differences between marbled crayfish and P. fallax females are highly significant (asterisks; P=2.06×10−5; Welsh two-sided t-test). Error bars represent s.d.
- Fig. 7.
Comparison of body size and fecundity between marbled crayfish and P. fallax. (A) Largest marbled crayfish from our laboratory having a total length of 10.3 cm. (B) Clutch of same specimen consisting of 731 eggs. (C) Differences in carapace length between populations of ovigerous marbled crayfish (mc) and P. fallax females (PF) from comparable climatic regions. Data for marbled crayfish (n=57) was obtained in Madagascar (Jones et al., 2009) and data for P. fallax (n=27) was obtained in Florida (Hendrix et al., 2000). Horizontal bars indicate ranges and vertical lines indicate mean values (m) and lower and upper range limits. The difference between marbled crayfish and P. fallax females is highly significant as indicated by the P-value. (D) Differences in clutch size between the same populations as in (C). The difference is highly significant as indicated by the P-value. For statistical calculations, the s.d. was taken as half the range, and a Bonferroni adjustment for multiplicity was applied.
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