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Figures
- Fig. 1.
The nucleotide and deduced protein sequences of leptin cDNA in the lined seahorse Hippocampus erectus. (A) Gene structure. The boxes represent coding exons. The numbers show the base pairs and amino acids (aa). (B) The signal peptide is shown in shadow. The cysteine residues used in disulfide linkages are circled.
- Fig. 2.
The molecular characterization of vertebrate leptin. (A) The comparison of amino acid sequences of the teleost and human leptins. The multiple sequence alignment was performed by ClustalX2.0. The signal peptides are indicated by a black triangle. The four α-helices of human leptin are boxed. The conserved cysteine residues involved in the formation of disulfide bridges are shaded. (B) The tertiary structures of seahorse and human leptins. The secondary and tertiary protein structures were modeled using the ProModII program at the SWISS-MODEL automated protein modeling server, based upon human leptin (1AX8.pdb) Protein Data Bank structure file.
- Fig. 3.
The tissue expression of leptin-a and lepr mRNA in seahorses (Hippocampus erectus). (A) Female and (B) male seahorses Hippocampus erectus (n=3). mRNA levels identified by RT-PCR normalized against β-actin transcript, including brain, gill, liver, intestine, kidney, muscle, testis (male), ovary (female), brood pouch (male), and skin (female) tissues.
- Fig. 4.
The expression of leptin-a and lepr mRNA of juvenile seahorses (Hippocampus erectus) under different food intake statuses for 7 days. (A) Liver, (B) brain (n=8). mRNA levels were qualified by real-time PCR. Asterisks denote significant differences between the different food intake statuses (P<0.05; one-way ANOVA followed by the Duncan's multiple-range tests).
- Fig. 5.
The mRNA expressions of leptin-a and lepr during different pregnant stages. (A)The mRNA levels were qualified by real-time PCR. The asterisks denote significant differences between pregnant and non-pregnant stages (P<0.05; one-way ANOVA followed by the Duncan's multiple-range tests; n=8). (B) The embryos attached to the brood pouch in the early pregnant stage while released to the brood pouch in the late pregnant stage.
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