Article Figures & Tables
Figures
- Fig. 1.
Microtubule polarity in the D-wing and P-abd. (A-E′) The fraction (A-E) or percentage (A′-E′) of Eb1::GFP comets observed moving in a given direction in wild-type (A, P=0.8984; A′); Pk-overexpressing (B, P=0.3013; B′); Sple-overexpressing (C, P=0.7578; C′); pk-sple mutant (D, P=0.7646; D′); and pk-sple mutant, Pk-overexpressing (E, P=0.8057; E′) D-wings. Genotypes for A,A′-E,E′ are numbers 1-5, respectively (see Materials and Methods). (F-J) The proportion (fraction) of Eb1::GFP comets observed moving in a given direction in wild-type (F, P=0.0273, 1808 comets from n=10 flies); Pk-overexpressing (G, P=0.7354; 1636 comets from n=13 flies); Sple-overexpressing (H, P=0.0398; 2131 comets from n=13 flies); pk-sple mutant (I, P=0.9097; 1839 comets from n=12 flies); and pk-sple mutant, Pk overexpressing (J, P=0.4233; 2184 comets from n=18 flies) P-abds. Genotypes for F-J are numbers 6-10, respectively. For A-E,F-J: grey lines link values from the same fly; blue bars mark the median; P-values are from a Wilcoxon matched-pairs signed rank test; *P<0.05. For A′-E′: n is the total number of comets with the number of flies is in parentheses; percentage is the proportion of comets moving towards the distal quadrant versus the proximal quadrant of angles; P-values are from a one-tailed binomial test. >> denotes overexpression.
- Fig. 2.
Dsh vesicles in the D-wing and P-abd. (A,B) Proportion (fraction) of Dsh::GFP vesicles observed moving in a given direction in D-wing (A, 126 total vesicles from 32 flies; P=0.00080; n=86 proximal and distal vesicles) and P-abd (B, 129 total vesicles from 56 flies; P=0.0098; n=98 proximal and distal vesicles). P-values from a one-tailed binomial test. (C) Average number of vesicles per cell per minute in each fly in the D-wing and P-abd. Error bars are s.d. **P<0.01; ***P<0.001. This figure uses genotype 14.
- Fig. 3.
Direction of hair growth and microtubule polarity in ft, d mutant abdomens. (A-C′) Abdominal segments from wild-type (A, genotype 17), ft-d null (B, genotype 18), and ft-d null, overexpressing Sple (C,C′, genotype 12) flies stained with phalloidin to visualize actin-rich prehairs. Yellow triangle indicates approximate location of anterior-posterior compartment boundary. HhGal4-driven UAS-Eb1::GFP expression marks the posterior compartment in C′. Scale bar=10 µm. >> denotes overexpression. (D-F) The proportion (fraction) of Eb1::GFP comets observed moving in a given direction in ft, d mutant A-abds (D, P=0.9697; 3471 comets from n=12 flies; genotype 13); ft, d mutant P-abds (E, P=0.3591; 2091 comets from n=15 flies; genotype 11); and ft, d mutant, Sple overexpressing P-abds (F, P=0.8160; 2077 comets from n=15 flies; genotype 12). Grey lines link values from the same fly; blue bars mark the median; P-values are from a Wilcoxon matched-pairs signed rank test.
Tables
Article Navigation
Related articles
Cited by...
More in this TOC section
Similar articles
Other journals from The Company of Biologists
First person interviews — Kim, Isabel and Antonio
In the latest interviews with early-career authors of our papers, Antonio Barral, Isabel Rollan and Kim Nguyen discuss their scientific backgrounds, research and future directions.
We're excited to be an affiliate journal for Review Commons, the ASAPbio/EMBO platform for high-quality journal-independent peer-review in the life sciences, which went live on 09 December.
On the cover of issue 11, Tawari, Wodarz et al. identify CG4038 as a gene downregulated in bam mutant ovaries in an RNA-seq transcriptome analysis.
Dates for 2020 — Grants, Meetings and Workshops
Get ahead and update your 2020 diary with our meeting grant deadlines! The Company of Biologists also has a fantastic line-up of Workshops with funded places for early-career researchers and larger Journal Meetings from our sister journals Development, Journal of Cell Science, Journal of Experimental Biology and Disease, Models & Mechanisms.
Why publish with BiO?
Biology Open aims to provide rapid publication for scientifically sound observations and valid conclusions in developmental, cell, experimental and translational biology. Submit your paper for an easy and fast publication process. Recent highlights in BiO by Maldonado, Morillo-Velarde et al. discuss Octopus insularis as a new marine model for evolutionary developmental biology.