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- Fig. 1.
Microtubule polarity in the D-wing and P-abd. (A-E′) The fraction (A-E) or percentage (A′-E′) of Eb1::GFP comets observed moving in a given direction in wild-type (A, P=0.8984; A′); Pk-overexpressing (B, P=0.3013; B′); Sple-overexpressing (C, P=0.7578; C′); pk-sple mutant (D, P=0.7646; D′); and pk-sple mutant, Pk-overexpressing (E, P=0.8057; E′) D-wings. Genotypes for A,A′-E,E′ are numbers 1-5, respectively (see Materials and Methods). (F-J) The proportion (fraction) of Eb1::GFP comets observed moving in a given direction in wild-type (F, P=0.0273, 1808 comets from n=10 flies); Pk-overexpressing (G, P=0.7354; 1636 comets from n=13 flies); Sple-overexpressing (H, P=0.0398; 2131 comets from n=13 flies); pk-sple mutant (I, P=0.9097; 1839 comets from n=12 flies); and pk-sple mutant, Pk overexpressing (J, P=0.4233; 2184 comets from n=18 flies) P-abds. Genotypes for F-J are numbers 6-10, respectively. For A-E,F-J: grey lines link values from the same fly; blue bars mark the median; P-values are from a Wilcoxon matched-pairs signed rank test; *P<0.05. For A′-E′: n is the total number of comets with the number of flies is in parentheses; percentage is the proportion of comets moving towards the distal quadrant versus the proximal quadrant of angles; P-values are from a one-tailed binomial test. >> denotes overexpression.
- Fig. 2.
Dsh vesicles in the D-wing and P-abd. (A,B) Proportion (fraction) of Dsh::GFP vesicles observed moving in a given direction in D-wing (A, 126 total vesicles from 32 flies; P=0.00080; n=86 proximal and distal vesicles) and P-abd (B, 129 total vesicles from 56 flies; P=0.0098; n=98 proximal and distal vesicles). P-values from a one-tailed binomial test. (C) Average number of vesicles per cell per minute in each fly in the D-wing and P-abd. Error bars are s.d. **P<0.01; ***P<0.001. This figure uses genotype 14.
- Fig. 3.
Direction of hair growth and microtubule polarity in ft, d mutant abdomens. (A-C′) Abdominal segments from wild-type (A, genotype 17), ft-d null (B, genotype 18), and ft-d null, overexpressing Sple (C,C′, genotype 12) flies stained with phalloidin to visualize actin-rich prehairs. Yellow triangle indicates approximate location of anterior-posterior compartment boundary. HhGal4-driven UAS-Eb1::GFP expression marks the posterior compartment in C′. Scale bar=10 µm. >> denotes overexpression. (D-F) The proportion (fraction) of Eb1::GFP comets observed moving in a given direction in ft, d mutant A-abds (D, P=0.9697; 3471 comets from n=12 flies; genotype 13); ft, d mutant P-abds (E, P=0.3591; 2091 comets from n=15 flies; genotype 11); and ft, d mutant, Sple overexpressing P-abds (F, P=0.8160; 2077 comets from n=15 flies; genotype 12). Grey lines link values from the same fly; blue bars mark the median; P-values are from a Wilcoxon matched-pairs signed rank test.
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