Article Figures & Tables
Figures
- Fig. 1.
Microtubule polarity in the D-wing and P-abd. (A-E′) The fraction (A-E) or percentage (A′-E′) of Eb1::GFP comets observed moving in a given direction in wild-type (A, P=0.8984; A′); Pk-overexpressing (B, P=0.3013; B′); Sple-overexpressing (C, P=0.7578; C′); pk-sple mutant (D, P=0.7646; D′); and pk-sple mutant, Pk-overexpressing (E, P=0.8057; E′) D-wings. Genotypes for A,A′-E,E′ are numbers 1-5, respectively (see Materials and Methods). (F-J) The proportion (fraction) of Eb1::GFP comets observed moving in a given direction in wild-type (F, P=0.0273, 1808 comets from n=10 flies); Pk-overexpressing (G, P=0.7354; 1636 comets from n=13 flies); Sple-overexpressing (H, P=0.0398; 2131 comets from n=13 flies); pk-sple mutant (I, P=0.9097; 1839 comets from n=12 flies); and pk-sple mutant, Pk overexpressing (J, P=0.4233; 2184 comets from n=18 flies) P-abds. Genotypes for F-J are numbers 6-10, respectively. For A-E,F-J: grey lines link values from the same fly; blue bars mark the median; P-values are from a Wilcoxon matched-pairs signed rank test; *P<0.05. For A′-E′: n is the total number of comets with the number of flies is in parentheses; percentage is the proportion of comets moving towards the distal quadrant versus the proximal quadrant of angles; P-values are from a one-tailed binomial test. >> denotes overexpression.
- Fig. 2.
Dsh vesicles in the D-wing and P-abd. (A,B) Proportion (fraction) of Dsh::GFP vesicles observed moving in a given direction in D-wing (A, 126 total vesicles from 32 flies; P=0.00080; n=86 proximal and distal vesicles) and P-abd (B, 129 total vesicles from 56 flies; P=0.0098; n=98 proximal and distal vesicles). P-values from a one-tailed binomial test. (C) Average number of vesicles per cell per minute in each fly in the D-wing and P-abd. Error bars are s.d. **P<0.01; ***P<0.001. This figure uses genotype 14.
- Fig. 3.
Direction of hair growth and microtubule polarity in ft, d mutant abdomens. (A-C′) Abdominal segments from wild-type (A, genotype 17), ft-d null (B, genotype 18), and ft-d null, overexpressing Sple (C,C′, genotype 12) flies stained with phalloidin to visualize actin-rich prehairs. Yellow triangle indicates approximate location of anterior-posterior compartment boundary. HhGal4-driven UAS-Eb1::GFP expression marks the posterior compartment in C′. Scale bar=10 µm. >> denotes overexpression. (D-F) The proportion (fraction) of Eb1::GFP comets observed moving in a given direction in ft, d mutant A-abds (D, P=0.9697; 3471 comets from n=12 flies; genotype 13); ft, d mutant P-abds (E, P=0.3591; 2091 comets from n=15 flies; genotype 11); and ft, d mutant, Sple overexpressing P-abds (F, P=0.8160; 2077 comets from n=15 flies; genotype 12). Grey lines link values from the same fly; blue bars mark the median; P-values are from a Wilcoxon matched-pairs signed rank test.
Tables
Article Navigation
Related articles
Cited by...
More in this TOC section
Similar articles
Other journals from The Company of Biologists
Cover article - Apolipoprotein L9 interacts with LC3/GABARAP and is a microtubule-associated protein with a widespread subcellular distribution
Arvind A. Thekkinghat, Kamlesh K. Yadav and Pundi N. Rangarajan demonstrate the versatile properties of apolipoprotein L9, a lipid-binding protein, and its influence on a variety of activities taking place inside an animal cell.
James J. Dowling and his colleagues conducted the first large-scale screening efforts for NEBLUN-related nemaline myopathy to address the urgent need for effective treatment for patients affected by the disease.
Have you seen our interviews with the early-career first authors of our papers? Recently, we caught up with first authors Arvind Thekkinghat, Shannon Taylor and Daisuke Takao.
Biology Open has strong credentials and publishing with us is easy and fast. BiO aims to provide rapid publication for scientifically sound observations and valid conclusions in developmental, cell, experimental and translational biology. Submit your paper here – you’ll be in good company!
Recent developmental biology highlights in BiO – Anna Nele Herdina and her colleagues define the spectrum of phenotypic abnormalities linked with Col4a2 disruption, and demonstrate the opportunities the Deciphering the Mechanisms of Developmental Disorders (DMDD) program offers for exploring rare human diseases.
Transfer to Biology Open
Did you know: if your submission to one of our sister journals, Development, Journal of Cell Science, Journal of Experimental Biology or Disease Models & Mechanisms, is unsuccessful, you can transfer your paper and any reviews directly to Biology Open. The majority of papers transferred with reviews are accepted for publication. Read here to find out more.
BiO partners with Publons.
Biology Open is pleased to announce a new partnership with Publons. This allows reviewers to easily track and verify every review by choosing to add the review to their Publons profile when completing the review submission form. Publons makes it simple for reviewers to showcase their peer review contributions in a format that can be included in job and funding applications (without breaking reviewer anonymity). Read the official announcement here!
preLights - preLights reaches 500 posts!
The success of preLights is down to the hard work of our preLighters. Head over to the preLights website to find out more about our most active early-career researchers.