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Research Article
Prickle isoforms control the direction of tissue polarity by microtubule independent and dependent mechanisms
Katherine A. Sharp, Jeffrey D. Axelrod
Biology Open 2016 5: 229-236; doi: 10.1242/bio.016162
Katherine A. Sharp
Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, L235, Stanford, CA 94305, USADepartment of Genetics, Stanford University School of Medicine, 300 Pasteur Drive, L235, Stanford, CA 94305, USA
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Jeffrey D. Axelrod
Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, L235, Stanford, CA 94305, USA
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  • For correspondence: jaxelrod@stanford.edu
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    Fig. 1.

    Microtubule polarity in the D-wing and P-abd. (A-E′) The fraction (A-E) or percentage (A′-E′) of Eb1::GFP comets observed moving in a given direction in wild-type (A, P=0.8984; A′); Pk-overexpressing (B, P=0.3013; B′); Sple-overexpressing (C, P=0.7578; C′); pk-sple mutant (D, P=0.7646; D′); and pk-sple mutant, Pk-overexpressing (E, P=0.8057; E′) D-wings. Genotypes for A,A′-E,E′ are numbers 1-5, respectively (see Materials and Methods). (F-J) The proportion (fraction) of Eb1::GFP comets observed moving in a given direction in wild-type (F, P=0.0273, 1808 comets from n=10 flies); Pk-overexpressing (G, P=0.7354; 1636 comets from n=13 flies); Sple-overexpressing (H, P=0.0398; 2131 comets from n=13 flies); pk-sple mutant (I, P=0.9097; 1839 comets from n=12 flies); and pk-sple mutant, Pk overexpressing (J, P=0.4233; 2184 comets from n=18 flies) P-abds. Genotypes for F-J are numbers 6-10, respectively. For A-E,F-J: grey lines link values from the same fly; blue bars mark the median; P-values are from a Wilcoxon matched-pairs signed rank test; *P<0.05. For A′-E′: n is the total number of comets with the number of flies is in parentheses; percentage is the proportion of comets moving towards the distal quadrant versus the proximal quadrant of angles; P-values are from a one-tailed binomial test. >> denotes overexpression.

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    Fig. 2.

    Dsh vesicles in the D-wing and P-abd. (A,B) Proportion (fraction) of Dsh::GFP vesicles observed moving in a given direction in D-wing (A, 126 total vesicles from 32 flies; P=0.00080; n=86 proximal and distal vesicles) and P-abd (B, 129 total vesicles from 56 flies; P=0.0098; n=98 proximal and distal vesicles). P-values from a one-tailed binomial test. (C) Average number of vesicles per cell per minute in each fly in the D-wing and P-abd. Error bars are s.d. **P<0.01; ***P<0.001. This figure uses genotype 14.

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    Fig. 3.

    Direction of hair growth and microtubule polarity in ft, d mutant abdomens. (A-C′) Abdominal segments from wild-type (A, genotype 17), ft-d null (B, genotype 18), and ft-d null, overexpressing Sple (C,C′, genotype 12) flies stained with phalloidin to visualize actin-rich prehairs. Yellow triangle indicates approximate location of anterior-posterior compartment boundary. HhGal4-driven UAS-Eb1::GFP expression marks the posterior compartment in C′. Scale bar=10 µm. >> denotes overexpression. (D-F) The proportion (fraction) of Eb1::GFP comets observed moving in a given direction in ft, d mutant A-abds (D, P=0.9697; 3471 comets from n=12 flies; genotype 13); ft, d mutant P-abds (E, P=0.3591; 2091 comets from n=15 flies; genotype 11); and ft, d mutant, Sple overexpressing P-abds (F, P=0.8160; 2077 comets from n=15 flies; genotype 12). Grey lines link values from the same fly; blue bars mark the median; P-values are from a Wilcoxon matched-pairs signed rank test.

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Keywords

  • Planar cell polarity
  • Prickle
  • Spiny-legs
  • Drosophila
  • Fat

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Research Article
Prickle isoforms control the direction of tissue polarity by microtubule independent and dependent mechanisms
Katherine A. Sharp, Jeffrey D. Axelrod
Biology Open 2016 5: 229-236; doi: 10.1242/bio.016162
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Research Article
Prickle isoforms control the direction of tissue polarity by microtubule independent and dependent mechanisms
Katherine A. Sharp, Jeffrey D. Axelrod
Biology Open 2016 5: 229-236; doi: 10.1242/bio.016162

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