Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Interviews
    • Sign up for alerts
  • About us
    • About BiO
    • Editors and Board
    • Editor biographies
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contact
    • Contact BiO
    • Advertising
    • Feedback
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in

Search

  • Advanced search
Biology Open
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Biology Open

Advanced search

RSS   Twitter   Facebook   YouTube

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Interviews
    • Sign up for alerts
  • About us
    • About BiO
    • Editors and Board
    • Editor biographies
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contact
    • Contact BiO
    • Advertising
    • Feedback
Research Article
Cell membrane disruption stimulates cAMP and Ca2+ signaling to potentiate cell membrane resealing in neighboring cells
Tatsuru Togo
Biology Open 2017 6: 1814-1819; doi: 10.1242/bio.028977
Tatsuru Togo
Department of Anatomy, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae, Kawasaki, Kanagawa 216-8511, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Tatsuru Togo
  • For correspondence: togot@marianna-u.ac.jp
  • Article
  • Figures & tables
  • Supp info
  • Info & metrics
  • eLetters
  • PDF + SI
  • PDF
Loading

Article Figures & Tables

Figures

  • Fig. 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 1.

    Scratching monolayers stimulates cAMP synthesis in MDCK cells. Confluent monolayers of MDCK cells were maintained in 1.8 mM Ca2+ Ringer's solution containing 100 µM IBMX, a phosphodiesterase inhibitor. Cells were then wounded by scratching monolayers with a 27G needle 20 times, or treated with 100 µM ATP or 100 µM forskolin. The amount of cAMP was quantified by immunoassay and normalized to the amount of total protein. The number of experiments is indicated in parentheses. *P=0.0006.

  • Fig. 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 2.

    Cell membrane disruption stimulates cAMP production in neighboring cells. (A) ‘w’ in the fluorescence image of MDCK cells expressing Green Upward cADDis indicates a wounded cell. Cells adjacent to the wounded cell were labeled with numbers in order of their proximity to the wounded cell. A cell was wounded at time zero with a glass needle in 1.8 mM Ca2+ Ringer's solution, and the time course of changes in fluorescence intensity of cADDis was plotted for neighboring cells (1–3). The image shown in this figure was acquired 90 s after cell membrane disruption. See also Movie 1. (B) Cells were wounded at time zero with a glass needle in the absence or presence of 20 U/ml apyrase, and changes in fluorescence intensity in neighboring cells were compared. The number of observed cells is indicated in parentheses. P=0.0007 (a–a′); P=0.0427 (b–b′); P=0.0197 (c–c′).

  • Fig. 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 3.

    ATP and forskolin stimulate cAMP synthesis in MDCK cells. Cells expressing Green Upward cADDis were treated with either 100 µM ATP or 100 µM forskolin at the time indicated by arrows, and the changes in fluorescence intensity of cADDis were recorded. The arrowhead indicates the transient decrease in fluorescence intensity. The number of observed cells is indicated in parentheses.

  • Fig. 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 4.

    Cell membrane disruption induces Ca2+ mobilization in neighboring MDCK cells. (A) Cells loaded with Calcium Green-1 AM were wounded at time zero with a glass needle in the presence or absence of extracellular Ca2+, and changes in fluorescence intensity in the cytoplasmic region were compared. Cells were numbered as per Fig. 2A. The number of observed cells is indicated in parentheses. See also Movie 2. (B) To compare the initial phase of increase in [Ca2+]i, data from cell #1 in A were expanded. *P=0.0333; NS, not significant.

  • Fig. 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 5.

    Cell membrane disruption potentiates membrane resealing in neighboring cells in a PKA- and PKC-dependent manner. Cells loaded with Calcein Red-Orange AM were initially wounded with a glass needle, and changes in fluorescence intensity of Calcein Red-Orange were monitored. Neighboring cells were wounded 5 min later and changes in fluorescence intensity were recorded. The resealing rate was defined as the reciprocal of resealing time in seconds. For cells that failed to reseal, the rate was defined as zero. The number of observed cells is indicated in parentheses. *P=0.002.

  • Fig. 6.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 6.

    Potentiation of membrane resealing induced by ATP is suppressed by PKA and PKC inhibitors in MDCK cells. Cells were pretreated for 10 min with either kinase inhibitors or DMSO. Then, cells were wounded with a glass needle after the addition of 100 µM ATP in the presence or absence of inhibitors, respectively, and the resulting resealing rates were analyzed. As a control, cells treated with 100 µM AMP were wounded with a glass needle. Resealing rates were analyzed 5–20 min after the addition of nucleotides. The number of observed cells is indicated in parentheses. *P=0.0007; †P<0.0001.

Previous ArticleNext Article
Back to top
Previous ArticleNext Article

This Issue

RSSRSS

Keywords

  • Membrane resealing
  • ATP
  • cAMP
  • Ca2+
  • Protein kinase A
  • Protein kinase C

 Download PDF

Email

Thank you for your interest in spreading the word on Biology Open.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Cell membrane disruption stimulates cAMP and Ca2+ signaling to potentiate cell membrane resealing in neighboring cells
(Your Name) has sent you a message from Biology Open
(Your Name) thought you would like to see the Biology Open web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Research Article
Cell membrane disruption stimulates cAMP and Ca2+ signaling to potentiate cell membrane resealing in neighboring cells
Tatsuru Togo
Biology Open 2017 6: 1814-1819; doi: 10.1242/bio.028977
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Research Article
Cell membrane disruption stimulates cAMP and Ca2+ signaling to potentiate cell membrane resealing in neighboring cells
Tatsuru Togo
Biology Open 2017 6: 1814-1819; doi: 10.1242/bio.028977

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article Navigation

  • Top
  • Article
    • ABSTRACT
    • INTRODUCTION
    • RESULTS
    • DISCUSSION
    • MATERIALS AND METHODS
    • Footnotes
    • References
  • Figures & tables
  • Supp info
  • Info & metrics
  • eLetters
  • PDF + SI
  • PDF

Related articles

Cited by...

More in this TOC section

  • The basal release of endothelium-derived catecholamines regulates the contractions of Chelonoidis carbonaria aorta caused by electrical-field stimulation
  • Smoking flies: Testing the effect of tobacco cigarettes on heart function of Drosophila melanogaster
  • Bisphenol A promotes stress granule assembly and modulates the integrated stress response
Show more RESEARCH ARTICLE

Similar articles

Other journals from The Company of Biologists

Development

Journal of Cell Science

Journal of Experimental Biology

Disease Models & Mechanisms

Advertisement

Biology Open and COVID-19

We are aware that the COVID-19 pandemic is having an unprecedented impact on researchers worldwide. The Editors of all The Company of Biologists’ journals have been considering ways in which we can alleviate concerns that members of our community may have around publishing activities during this time. Read about the actions we are taking at this time.

Please don’t hesitate to contact the Editorial Office if you have any questions or concerns.


2020 at The Company of Biologists

Despite 2020’s challenges, we achieved a lot at The Company of Biologists. In the midst of the pandemic, we have seen long-term projects and new ventures come to fruition. Read our full lowdown of 2020.


Interview- Sebastian Markert

Sebastian Markert is first author of a paper in BiO using C. elegans to model amyotrophic lateral sclerosis. In an interview, he talks about the potential implications of his work and his future plans.


Three communities to support biologists to everywhere

Online communities have never been more important. If you’re looking for somewhere to meet fellow scientists, take part in topical discussions and find virtual events in your field, take a look at each of our community sites:

  • The Node: the community site for and by developmental biologists
  • preLights: the preprint highlights service run by the biological community
  • FocalPlane: the community site for microscopists and biologists alike

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Interviews
  • Sign up for alerts

About us

  • About BiO
  • Editors and Board
  • Editor biographies
  • Grants and funding
  • Journal Meetings
  • Workshops
  • The Company of Biologists

For Authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Article types
  • Manuscript preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access

Journal Info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Sign up for alerts

Contact

  • Contact BiO
  • Advertising
  • Feedback

Twitter   YouTube   LinkedIn

© 2021   The Company of Biologists Ltd   Registered Charity 277992