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METHODS & TECHNIQUES
Parallel in vivo monitoring of pH in gill capillaries and muscles of fishes using microencapsulated biomarkers
Ekaterina Borvinskaya, Anton Gurkov, Ekaterina Shchapova, Boris Baduev, Zhanna Shatilina, Anton Sadovoy, Igor Meglinski, Maxim Timofeyev
Biology Open 2017 6: 673-677; doi: 10.1242/bio.024380
Ekaterina Borvinskaya
1Institute of Biology at Irkutsk State University, Irkutsk 664003, Russia
2Institute of Biology at Karelian Research Centre of Russian Academy of Sciences, Petrozavodsk 185035, Russia
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Anton Gurkov
1Institute of Biology at Irkutsk State University, Irkutsk 664003, Russia
3Baikal Research Centre, Irkutsk 664003, Russia
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Ekaterina Shchapova
1Institute of Biology at Irkutsk State University, Irkutsk 664003, Russia
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Boris Baduev
1Institute of Biology at Irkutsk State University, Irkutsk 664003, Russia
3Baikal Research Centre, Irkutsk 664003, Russia
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Zhanna Shatilina
1Institute of Biology at Irkutsk State University, Irkutsk 664003, Russia
3Baikal Research Centre, Irkutsk 664003, Russia
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Anton Sadovoy
4Institute of Materials Research and Engineering, A*STAR, 138634, Singapore
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Igor Meglinski
1Institute of Biology at Irkutsk State University, Irkutsk 664003, Russia
5University of Oulu, Optoelectronics and Measurement Techniques Laboratory, Oulu 90570, Finland
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Maxim Timofeyev
1Institute of Biology at Irkutsk State University, Irkutsk 664003, Russia
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  • ORCID record for Maxim Timofeyev
  • For correspondence: m.a.timofeyev@gmail.com
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ABSTRACT

Tracking physiological parameters in different organs within the same organism simultaneously and in real time can provide an outstanding representation of the organism's physiological status. The state-of-the-art technique of using encapsulated fluorescent molecular probes (microencapsulated biomarkers) is a unique tool that can serve as a platform for the development of new methods to obtain in vivo physiological measurements and is applicable to a broad range of organisms. Here, we describe a novel technique to monitor the pH of blood inside the gill capillaries and interstitial fluid of muscles by using microencapsulated biomarkers in a zebrafish model. The functionality of the proposed technique is shown by the identification of acidification under anesthesia-induced coma and after death. The pH in muscles reacts to hypoxia faster than that in the gill bloodstream, which makes both parameters applicable as markers of either local or bodily reactions.

Footnotes

  • Competing interests

    The authors declare no competing or financial interests.

  • Author contributions

    Conceptualization: E.B., A.G., B.B., A.S., I.M., M.T.; Methodology: E.B., A.G., E.S., B.B., I.M.; Software: E.B., A.G., E.S., A.S.; Formal analysis: A.G., Z.S., A.S.; Investigation: E.B., A.G., E.S.; Resources: E.B., A.G., B.B.; Data curation: E.B., A.G.; Writing – original draft: E.B., A.G.; Writing – review & editing: M.T., Z.S., I.M.; Visualization: E.B., A.G., E.S.; Supervision: I.M., M.T.; Project administration: I.M., M.T.; Funding acquisition: I.M., M.T.

  • Funding

    This research was supported by the Russian Science Foundation (15-14-10008).

  • Received February 15, 2017.
  • Accepted March 28, 2017.
  • © 2017. Published by The Company of Biologists Ltd
http://creativecommons.org/licenses/by/3.0

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Keywords

  • Blood pH
  • Encapsulated fluorescent sensors
  • Interstitial pH
  • Microencapsulated biomarkers
  • Physiological measurements in vivo
  • Zebrafish

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METHODS & TECHNIQUES
Parallel in vivo monitoring of pH in gill capillaries and muscles of fishes using microencapsulated biomarkers
Ekaterina Borvinskaya, Anton Gurkov, Ekaterina Shchapova, Boris Baduev, Zhanna Shatilina, Anton Sadovoy, Igor Meglinski, Maxim Timofeyev
Biology Open 2017 6: 673-677; doi: 10.1242/bio.024380
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METHODS & TECHNIQUES
Parallel in vivo monitoring of pH in gill capillaries and muscles of fishes using microencapsulated biomarkers
Ekaterina Borvinskaya, Anton Gurkov, Ekaterina Shchapova, Boris Baduev, Zhanna Shatilina, Anton Sadovoy, Igor Meglinski, Maxim Timofeyev
Biology Open 2017 6: 673-677; doi: 10.1242/bio.024380

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