Advertisement
Research Article
A Gata4 nuclear GFP transcriptional reporter to study endoderm and cardiac development in the mouse
Claire S. Simon, Lu Zhang, Tao Wu, Weibin Cai, Nestor Saiz, Sonja Nowotschin, Chen-Leng Cai, Anna-Katerina Hadjantonakis
Biology Open 2018 7: bio036517 doi: 10.1242/bio.036517 Published 10 December 2018

ABSTRACT

The GATA zinc-finger transcription factor GATA4 is expressed in a variety of tissues during mouse embryonic development and in adult organs. These include the primitive endoderm of the blastocyst, visceral endoderm of the early post-implantation embryo, as well as lateral plate mesoderm, developing heart, liver, lung and gonads. Here, we generate a novel Gata4 targeted allele used to generate both a Gata4H2B-GFP transcriptional reporter and a Gata4FLAG fusion protein to analyse dynamic expression domains. We demonstrate that the Gata4H2B-GFP transcriptional reporter faithfully recapitulates known sites of Gata4 mRNA expression and correlates with endogenous GATA4 protein levels. This reporter labels nuclei of Gata4 expressing cells and is suitable for time-lapse imaging and single cell analyses. As such, this Gata4H2B-GFP allele will be a useful tool for studying Gata4 expression and transcriptional regulation.

This article has an associated First Person interview with the first author of the paper.

INTRODUCTION

GATA4 is a member of the highly conserved GATA family zinc-finger transcription factors (Viger et al., 2008), which bind to the GATA motif consensus sequence 5-AGATAG-3 in promoter and cis-regulatory elements of target genes to activate transcription (Arceci et al., 1993; Merika and Orkin, 1993). The GATA factors can be subdivided into two families based on phylogenetic analysis and their tissue expression profiles; the hematopoietic factors: GATA1/2/3, and the endodermal and cardiac factors: GATA4/5/6 (Crispino and Horwitz, 2017; Peterkin et al., 2005; Lentjes et al., 2016). In humans, GATA4 mutations are associated with a wide range of congenital heart defects including ventricular septal defects and gonadal anomalies (Garg et al., 2003; Pehlivan et al., 1999; Lourenco et al., 2011; Wang et al., 2011).

In mice, Gata4 plays an important role in the development of extra-embryonic endoderm. During pre-implantation development, from embryonic day (E) ∼3.5 to ∼E4.5, the inner cell mass (ICM) of the blastocyst gives rise to the pluripotent epiblast (Epi) and the extra-embryonic primitive endoderm (PrE) (Rossant, 2016; Chazaud and Yamanaka, 2016). While other PrE markers GATA6, PDGFRA, SOX17 are expressed to some extent in uncommitted ICM cells, GATA4 is the earliest known PrE-specific marker (Artus et al., 2011; Plusa et al., 2008; Kurimoto et al., 2006; Arceci et al., 1993; Niakan et al., 2010; Morris et al., 2010; Chazaud et al., 2006; Ohnishi et al., 2014), making it a prime candidate reporter for the identification of PrE cells as they emerge in real-time. Overexpression of either GATA4 or GATA6 is sufficient to induce differentiation of pluripotent embryonic stem (ES) cells to extra-embryonic endoderm like (XEN) cells (Fujikura et al., 2002; Schroter et al., 2015; Morrisey et al., 1996; Shimosato et al., 2007; Niakan et al., 2013), although Gata4 is dispensable for PrE specification in vivo (Molkentin et al., 1997; Kuo et al., 1997).

Gata4 is functionally required later in the extra-embryonic endoderm at early post-implantation stages, where it is expressed in the parietal (ParE) and visceral (VE) endoderm (Heikinheimo et al., 1994; Arceci et al., 1993; Cai et al., 2008). A third of Gata4−/− embryos fail to gastrulate, causing early lethality at ∼E7.5 (Molkentin et al., 1997). The remaining two thirds of Gata4−/− embryos arrest at ∼E9.0 due to a failure in ventral closure leading to defective heart morphogenesis (Molkentin et al., 1997; Kuo et al., 1997). These phenotypes can be rescued with wild-type extra-embryonic tissues (Narita et al., 1997; Watt et al., 2004, 2007), indicating that abnormal VE development is the primary defect in both classes of Gata4−/− embryos. In support of Gata4 playing a key role in VE development, Gata4−/− ES cells fail to efficiently differentiate into VE-like cells (Soudais et al., 1995).

Gata4 also plays essential roles in cardiac development (Pikkarainen et al., 2004; Peterkin et al., 2005; Zhao et al., 2008). Tetraploid complementation with Gata4−/− ES cells produce embryos with abnormal heart tube looping and thin-walled myocardium (Watt et al., 2007, 2004). Disruption of Gata4 function in the heart by either tissue-specific ablation, impaired FOG2 interaction, or substitution with Gata6, all result in severe cardiac abnormalities (Zeisberg et al., 2005; Oka et al., 2006; Crispino et al., 2001; Rojas et al., 2008; Rivera-Feliciano et al., 2006; Borok et al., 2016). GATA4 therefore is considered a key regulator of cardiogenesis, and Gata4 mRNA is broadly expressed throughout cardiac development (Auda-Boucher et al., 2000; Arceci et al., 1993). During gastrulation, Gata4 transcripts are detected in the cardiac and lateral plate mesoderm (Heikinheimo et al., 1994; Rojas et al., 2005; Schachterle et al., 2012). Gata4 expression continues in the primitive heart tube from E8.0 in both the endocardium and myocardium, and persists in cardiomyocytes throughout embryonic development and into adulthood (Heikinheimo et al., 1994; Morrisey et al., 1996; Schachterle et al., 2012). In addition to its roles in VE and cardiac morphogenesis, Gata4 expression is also required for proper development of the ovary, testes, pancreas, lung and liver (Kyrönlahti et al., 2011a,b; Watt et al., 2007).

Due to the pivotal roles of Gata4 in a variety of tissues, visualising the dynamic spatial and temporal expression domains is of great importance. Tissue-specific sites of Gata4 expression have been recapitulated with cis-regulatory elements driving transgenic LacZ reporters, for example in the lateral mesoderm (G2 enhancer), cardiac lineage (G9 enhancer) and the endoderm (G4 and G8 enhancers) (Rojas et al., 2005, 2009, 2010; Schachterle et al., 2012). Endogenous sites Gata4 expression can be replicated with rGata4p-[5kb]GFP and rGata4p-[5kb]RFP transgenic reporters which contain a 5kb fragment of the rat Gata4 proximal promoter (Pilon et al., 2008; Mazaud Guittot et al., 2007). However, single-cell resolution image analysis of rGata4p-[5kb]GFP and rGata4p-[5kb]RFP is challenging due to the cytoplasmic localisation of the fluorescence proteins used in these reporters.

Here, to circumvent transgenic site-specific and copy-number integration effects on reporter expression, and to conserve all cis-regulatory sequences, we generated a novel nuclear localised fluorescent reporter and a FLAG fusion targeted to the endogenous Gata4 locus. The transcriptional reporter, Gata4H2B-GFP, faithfully recapitulated known sites of Gata4 expression and strongly correlated with GATA4 protein levels. GFP expression was robustly observed in the endoderm lineages of the PrE, VE and midgut. In addition, GFP was also expressed in mesodermal cell types; early extra-embryonic mesoderm, cardiac lineage, lateral plate mesoderm, septum transversum, and then later in the lung and hepatic mesenchyme. This reporter brightly labelled nuclei of Gata4 expressing cells and was suitable for time-lapse imaging and single-cell resolution analysis. In addition, Cre recombination of the Gata4H2B-GFP allele generates a Gata4FLAG allele encoding a fusion protein for use in biochemical studies. As such, the Gata4H2B-GFP and Gata4FLAG alleles, represents useful tools for identifying novel sites of Gata4 expression and yield insights into how this key transcription factor is regulated and functions during embryonic development and tissue morphogenesis.

RESULTS AND DISCUSSION

Generation of a Gata4H2B-GFP reporter

To follow transcriptional activity of Gata4 in mouse embryonic development, we generated a bright nuclear localised fluorescent reporter that would be suitable for live imaging and for single-cell resolution analysis. For this, we used a histone-fused GFP protein H2B-GFP (Kanda et al., 1998; Hadjantonakis and Papaioannou, 2004). To circumvent site-specific integration effects of transgenes on reporter expression, and to conserve all cis-regulatory sequences we engineered a targeted allele at the endogenous Gata4 locus.

The Gata4H2B-GFP/+ knock-in mouse model was generated by inserting a loxP-flanked H2B-GFP-4×PolyA cassette, followed by a FRT-flanked Neo selection cassette, and a 3×FLAG tag into the endogenous ATG start site of Gata4 exon 2. This was achieved by homologous recombination in mouse embryonic stem (ES) cells (Fig. 1A). Correctly targeted ES cells were screened by long-range PCR (Fig. 1B), and were then used to generate germline chimeric mice. The FRT flanked Neo cassette was excised in vivo by crossing with a ubiquitously expressing FLP mouse line to generate the Gata4H2B-GFP allele. The H2B-GFP-4×PolyA sequence upstream of the Gata4 coding sequence renders the Gata4H2B-GFP a null allele, and no homozygous mice could be recovered at weaning age (Table 1).

Fig. 1.
Fig. 1.

Generation of Gata4H2B-GFP/+ targeted mouse model. (A) The cassette LoxP-H2B-GFP-4XPloyA-LoxP-FRT-Neo-FRT-3XFLAG was inserted into the start codon of the mouse Gata4 locus through homologous recombination. The Neo cassette was flanked by two FRT sites and removed by crossing to Flippase mice to generate the Gata4H2B-GFP allele. Cre excision of H2B-GFP generates GATA4-FLAG N-terminal protein fusion Gata4FLAG allele. (B) The targeted ES cells were confirmed by long-range PCR amplification of two fragments using primers P1 and P2, P3 and P4, respectively. Primers P1 and P2 produced a 4.3 kb band. Primers P3 and P4 produced a 4.9 kb band. (C) Western blot of individual E7.5 embryo littermates from a Gata4FLAG/+×wild-type mating. The anti-FLAG antibody cross reacts with GATA4-FLAG in Gata4FLAG/+ embryos. Total protein lysis of an ear from a Gata4FLAG/+ adult male was used as a positive control.

View this table:
Table 1.

Genotype of offspring from Gata4H2B-GFP/+×Gata4H2B-GFP/+ matings

Cre-mediated excision of the LoxP-flanked H2B-GFP-4×PolyA generated a Gata4FLAG allele that encodes an N-terminal 3×FLAG tagged GATA4 fusion protein. Western blot of individual E7.5 littermate embryos from a Gata4FLAG/+ and wild-type mating demonstrated GATA4-FLAG protein expression in those embryos that presumably inherited the Gata4Flag allele. The GATA4-FLAG fusion protein had an approximate molecular weight of 47 kDa, in agreement with its predicted size. Gata4FLAG heterozygous and homozygous mice were viable and fertile (Table S1). This suggests that the GATA4-FLAG protein fusion does not markedly disrupt GATA4 protein function, due to the absence of early embryonic lethality that is seen in Gata4 null mice.

Primitive endoderm cells express Gata4H2B-GFP reporter at the blastocyst stage

Uncommitted ICM cells of the blastocyst co-express the transcription factors NANOG and GATA6, a pattern that is gradually and asynchronously resolved as cells become specified between the 32–100 cell stages to NANOG+ Epi cells and GATA6+ PrE cells (Saiz et al., 2016b; Plusa et al., 2008; Chazaud et al., 2006). PrE specification and lineage maturation follows a sequential, hierarchical activation of the PrE lineage-specific markers; GATA6, PDGFRA, SOX17, GATA4 and SOX7 (Plusa et al., 2008; Arceci et al., 1993; Artus et al., 2011; Kurimoto et al., 2006; Niakan et al., 2010; Morris et al., 2010; Chazaud et al., 2006). Mature PrE cells express GATA4 from ∼58–63 cell stage blastocyst (Plusa et al., 2008).

To determine when and where the Gata4H2B-GFP reporter is expressed during pre-implantation development, we directly visualised GFP and stained for GATA4 protein in fixed heterozygous embryos between early-, (32–64 cells), mid- (64–100 cells) and late- (>100 cells) blastocyst stages by laser scanning confocal microscopy (Fig. 2A). Nuclear fluorescent intensities were quantified using a MATLAB-based nuclear segmentation algorithm, MINS (Lou et al., 2014). The expression of the Gata4H2B-GFP reporter was tightly correlated with GATA4 antibody staining in the ICM across pre-implantation development (r=0.89; Fig. 2B, Fig. S1A). Although occasionally we observed GFP positive cells that did not show nuclear GATA4 staining (Fig. 2A), likely due to H2B-GFP remaining bound to chromatin in mitotic cells or possibly independent regulation of Gata4 transcription and GATA4 protein levels. Gata4H2B-GFP expression was observed in ∼62 cell stage embryos that had specified few GATA4+ PrE cells and levels increased over-time within the PrE (Fig. S1A). By the late blastocyst stage (>100 cells) Gata4H2B-GFP expression was spatially restricted to the PrE epithelial layer lining the blastocyst cavity (Fig. 2A). Similar results were also seen using an alternative GATA4 antibody and anti-GFP antibody (Fig. S1B)

Fig. 2.
Fig. 2.

Gata4H2B-GFP expression marks primitive endoderm during pre-implantation development. (A) Representative images showing 10 μm maximum intensity projections of confocal z-stack images of fixed Gata4H2B-GFP/+ embryos immunostained for GATA4 (rabbit antibody) at E3.5–E4.5 stages of pre-implantation development. GFP signal was detected directly and nuclei were stained with Hoechst. Quantification shown in B and Fig. S1A. (B) Scatter plot showing the nuclear fluorescence intensity of GFP and endogenous GATA4 protein within individual ICM cells of Gata4H2B-GFP/+ embryos (n=20) between E3.5–E4.5 stages. Pearson correlation coefficient (r) and P values are shown in the graph. (C) Representative images showing 10 μm maximum intensity projections of confocal z-stack images of Gata4H2B-GFP/+ embryos fixed and immunostained with GFP, GATA6 and NANOG at E3.5–E4.5 stages of pre-implantation development. Nuclei were stained with Hoechst. Quantification and lineage allocation shown in D, E and Fig. S1B. (D) Boxplots showing the GFP nuclear fluorescence intensity in primitive endoderm (PrE, GATA6+, blue), double positive (DP, GATA6+NANOG+, purple), epiblast (EPI, NANOG+, red) and double negative (DN, GATA6−NANOG−, grey) ICM cells of fixed Gata4H2B-GFP/+ embryos (n=19). (E) Scatter plot showing the relative nuclear fluorescence intensity of NANOG and GATA6, coloured according to GFP nuclear intensity within individual ICM cells of fixed Gata4H2B-GFP/+ embryos (n=19 embryos). (F) Still images of time-lapse microscopy of E3.5 Gata4H2B-GFP/+ embryos with GFP overlaid on bright field (BF) or GFP alone. GFP+ cells emerge from a deep position (arrow) within the ICM and sort onto the surface of the blastocoel cavity. Blastocyst embryo stages represented by cell (c) number. Fixed embryo scale bars: 20 μm; time-lapse scale bars: 50 μm.

To confirm the association of Gata4H2B-GFP expression to the PrE, we compared GFP levels between ICM lineages in NANOG and GATA6 stained embryos. Cell identities were assigned as previously described (Saiz et al., 2016b,a), as either PrE (GATA6+), EPI (NANOG+), double positive (DP, NANOG+GATA6+) or double negative (DN, NANOG–GATA6–) (Fig. 2C, Fig. S1C). GFP levels were enriched in PrE (GATA6+NANOG−) cells, and increased in expression in later stage embryos (>90 cells, Fig. 2D,E). Gata4H2B-GFP expressing cells also co-stained for the PrE markers SOX7 and SOX17 (Fig. S1D,E).

To assess the dynamics of the Gata4H2B-GFP reporter, we performed time-lapse imaging of pre-implantation embryos. Mid-blastocyst stage Gata4H2B-GFP/+ embryos (∼E3.5) were imaged for 16 h. The Gata4H2B-GFP reporter was sufficiently bright to follow the trajectory of PrE cells emerging within the ICM to the surface of the blastocoel cavity (Fig. 2F, arrow and Movie 1). To experimentally estimate the half-life of Gata4H2B-GFP we treated late blastocyst stage (∼E4.0) embryos for 12 h with low levels of cycloheximide (10 μM) to partially inhibit protein translation (Fig. S2A and Movies 2 and 3). The half-life of the exponential decay rate of H2B-GFP under cycloheximide treatment was approximately 5.5 h (Fig. S2B,C), similar to the 6 h half-life observed for Nanog:H2B-GFP (Xenopoulos et al., 2015). Together, these data demonstrate the Gata4H2B-GFP reporter recapitulates endogenous gene expression, faithfully marking the PrE lineage, and can be used to visualise PrE emergence in live embryos.

Early post-implantation expression of Gata4H2B-GFP in the visceral endoderm and mesoderm

The PrE lineage gives rise to the ParE and VE populations of the post-implantation embryo, which both express Gata4 transcripts (Heikinheimo et al., 1994; Arceci et al., 1993; Morrisey et al., 1998). To determine the expression of Gata4H2B-GFP in these extra-embryonic lineages at early post-implantation stages, we directly visualised GFP expression and stained for endogenous GATA4 protein in E5.5 and E6.0 Gata4H2B-GFP/+ heterozygous embryos. At E5.5 and at E6.0, prior to overt primitive streak formation (pre-streak; PS stage), GATA4 protein was expressed throughout the embryonic visceral endoderm (emVE) and extra-embryonic visceral endoderm (exVE) (Cai et al., 2008), where it co-localised with GFP expression (Fig. 3A,B). At both E5.5 and E6.0 GFP expression was absent from the epiblast and extra-embryonic ectoderm (ExE) (Fig. 3A,B). Together, these data demonstrate that the Gata4H2B-GFP allele faithfully recapitulates previously described expression domains of Gata4 in the VE prior to gastrulation (Cai et al., 2008; Morrisey et al., 1998; Heikinheimo et al., 1994).

Fig. 3.
Fig. 3.

The Gata4H2B-GFP reporter is expressed in the visceral endoderm at early post-implantation stages. (A–B) Representative confocal images of fixed whole-mount Gata4H2B-GFP/+ embryos immunostained for endogenous GATA4 protein at E5.5 (A) and E6.0 (B) post-implantation stages prior to gastrulation. Upper panels show maximum intensity projection (MIP) and lower panels show sagittal views of projected 10um z-stack (A) or single optical plane (B). Nuclei are stained with Hoechst and GFP was visualised directly. GFP and GATA4 are co-localised in extra-embryonic visceral endoderm (exVE) and embryonic visceral endoderm (emVE) but absent in extra-embryonic ectoderm (exe) and epiblast (epi). Scale bars: 20 μm. Ectoplacental cone (EPC), anterior (A), posterior (P), proximal (Pr), distal (D).

At gastrulation (E6.5–E7.25), cells of the proximal-posterior epiblast undergo an epithelial-to-mesenchymal transition (EMT) in the primitive streak region to form the mesoderm and definitive endoderm (DE), and migrate between the epiblast and VE epithelial layers (Arnold and Robertson, 2009; Viotti et al., 2014a). By early bud stage (E7.5) DE cells intercalate into and disperse the overlying emVE cell layer (Kwon et al., 2008; Viotti et al., 2014b).

To assess Gata4H2B-GFP expression during gastrulation we visualised GFP and GATA4 protein in E6.25–E7.75 Gata4H2B-GFP/+ embryos. At the early- and mid-streak stage (E6.25–E6.5) GFP is expressed throughout emVE and exVE cells, where it is largely co-expressed with GATA4 protein in both the exVE and emVE (Fig. 4A, Fig. S3A). The GFP+ emVE cells overlying the anterior region of the epiblast expressed the highest levels of GATA4, however, in the posterior region of the emVE, we observed GFP positive cells that did not show nuclear GATA4 staining (Fig. 4A). The broader domain of Gata4H2B-GFP expression may be due independent regulation of transcript and protein levels, or the perdurance of the GFP protein. At the early bud stage and early head fold stage (E7.5–E7.75), after emVE dispersal, the sparse emVE cells overlying the epiblast expressed GFP (Fig. 4B, Fig. S3B). Together, these data suggest that at gastrulation stages Gata4H2B-GFP marks the entire VE (both exVE and emVE), both before and after emVE dispersal.

Fig. 4.
Fig. 4.

Gata4H2B-GFP/+ reporter expression marks the visceral endoderm, cardiac and lateral plate mesoderm at gastrulation stages. (A–B) Representative confocal images of fixed whole-mount Gata4H2B-GFP/+ embryos immunostained for endogenous GATA4 protein at E6.5 (A) and E7.5 (B) post-implantation stages during gastrulation. Upper panels show maximum intensity projection (MIP) and lower panels show sagittal views of a single optical plane (B). Nuclei are stained with Hoechst and GFP was visualised directly. (A) GFP expression throughout the embryonic and extra-embryonic visceral endoderm (emVE and exVE) and co-localisation with endogenous GATA4 protein. (B) After definitive endoderm intercalation and emVE dispersal, GFP+ emVE cells remain dispersed over the epiblast. Upper panels show maximum intensity projection (MIP) and lower panels show sagittal views of a single optical plane. (C) Representative confocal images of fixed whole-mount Gata4H2B-GFP/+ embryos immunostained for the primitive streak (ps) and nascent mesoderm marker BRACHYURY (T) at E7.25. Upper panels show maximum intensity projection (MIP) and lower panels show sagittal views of a single optical plane. (a,b) Transverse section at the positions indicated on MIP images. (a) Transverse section though the extra-embryonic (Ex-Em) region showing high levels of GFP fluorescence in the exVE, with lower levels of GFP fluorescence in extra-embryonic mesoderm. (b) Transverse section though the embryonic (Em) region showing lower levels of GFP fluorescence in the embryonic mesoderm, likely cardiac and lateral plate mesoderm, and emVE. Boxed regions on the sagittal view (c) show higher magnification of anterior region with GFP+ cells in the mesoderm layer, and (d) higher magnification of the posterior primitive streak region with GFP expression only present in the emVE. Scale bars: 50 μm. Anterior (A), posterior (P), proximal (Pr), distal (D), mesoderm (mes, or m), epiblast (epi), extra-embryonic ectoderm (exe), allantois (al).

At the early bud stage and early head fold stage (E7.5–E7.75), GFP+GATA4+ cells were observed in the mesoderm layer (Fig. 4B, Fig. S3B). To better identify Gata4H2B-GFP reporter expression within cells of the mesoderm, we stained Gata4H2B-GFP/+ late steak (∼E7.25) embryos for BRACHYURY (T) to mark the primitive streak and nascent mesoderm (Fig. 4C). Transverse cryo-sections through the extra-embryonic region showed high levels of GFP expression in the exVE, and lower GFP levels in the extra-embryonic mesoderm (Fig. 4Ca), in agreement with known sites of GATA4 expression in extra-embryonic endoderm and mesoderm (Nemer and Nemer, 2003).

Transverse sections through the primitive streak revealed GFP expression in the emVE, and the anterior and lateral migrating mesoderm layer (Fig. 4Cb), likely representing cardiac and lateral plate mesoderm (Rojas et al., 2005; Schachterle et al., 2012; Zhao et al., 2008). T expression in the nascent mesoderm was restricted to the posterior region of the embryo, and did not coincide with GFP expression (Fig. 4Cb). Reciprocal expression of T and GFP in the posterior and anterior mesoderm was also seen in sagittal single optical views of whole-mount embryos (Fig. 4Cc,d). These data suggest that as cardiac mesoderm and lateral mesoderm cells migrate away from the streak they downregulate T and activate Gata4 expression, similar to the pattern of T/Gata6 at this stage (Freyer et al., 2015).

Gata4H2B-GFP expression at mid-gestation

To follow Gata4H2B-GFP expression at later stages, we first visualised whole-mount heterozygous E8.5 embryos under epi-fluorescence and observed GFP expression in the primitive heart (Fig. 5). To assess tissue-specific expression, embryos were stained for GATA4 protein, cryo-sectioned and imaged by confocal microscopy. At this stage, GFP expression was detected in the right and left ventricle of the primitive heart tube, in both the outer myocardium and the inner endocardium (Fig. 5A). The sinus venosus, precursor of the atria, was also positive for GFP (Fig. 5B). GATA4 protein expression overlapped with GFP in the primitive heart tube, although we noted lower levels of GATA4 protein in the region separating the left and right ventricles. Co-expression of GFP and GATA4 was also observed in the septum transversum mesenchyme of lateral mesoderm origin (Fig. 5B). The exVE derived yolk sac endoderm and anterior midgut co-expressed both GFP and GATA4 while the endoderm of the foregut, posterior midgut and hindgut was negative for both GFP and GATA4 protein at this stage (Fig. 5A,B, Fig. S4).

Fig. 5.
Fig. 5.

Gata4H2B-GFP/+ reporter expression at E8.5. Left panels show lateral whole-mount Gata4H2B-GFP/+ embryos at E8.5 prior to fixation with GFP overlaid over bright field or GFP alone. (A,B) Transverse sections of whole-mount stained Gata4H2B-GFP/+ embryos for endogenous GATA4 protein. GFP was visualised directly and DNA was stained with Hoechst at positions indicated on whole-mount embryo. (A,A′) GFP expression is observed throughout the endocardium (inner) and myocardium (outer) layers of the heart and is co-expressed with GATA4 protein (B,B′) and also in the sinus venosus, septum transversum (st) and yolk sac (YS) endoderm. Scale bars: 100 μm. Neural tube (NT), foregut (fg), right (R), left (L), dorsal (D), ventral (V), anterior (A), posterior (P), right ventricle (rv), left ventricle (lv). Arrowheads indicate myocardial wall and asterisk indicates endocardium.

At E9.5 Gata4H2B-GFP/+ embryos showed continued GFP expression within the heart. Sections of E9.5 Gata4H2B-GFP/+ embryos revealed GFP+ cells throughout the endocardium and myocardium of the heart (Fig. 6Aa), in agreement with Gata4 being expressed throughout cardiac development as a key regulator of cardiogenesis (Arceci et al., 1993; Heikinheimo et al., 1994). In addition, GFP was expressed in the septum transversum at E8.5, but was absent in the adjacent foregut endoderm at E9.5 (Fig. 6Ab). While both the foregut and hindgut endoderm remained negative for GFP, the midgut endoderm robustly expressed Gata4H2B-GFP at this stage.

Fig. 6.
Fig. 6.

Gata4H2B-GFP/+ reporter expression at midgestation. (A) Upper panel showing lateral whole-mount immunofluorescence of Gata4H2B-GFP/+ embryos at E9.5. GFP is overlaid over bright field or GFP alone. (a-c) Transverse sections of an E9.5 Gata4H2B-GFP/+ embryo at indicated positions on whole-mount embryo. GFP was visualised directly and DNA was stained with Hoechst (a) GFP continues to be expressed throughout the heart (b) and the septum transversum. (c) GFP is also expressed in the midgut (mg) epithelium at this stage but is absent from the foregut and hindgut. (B) Lateral whole-mount immunofluorescence of Gata4H2B-GFP/+ embryos at E12.5. GFP is overlaid over bright field or GFP alone. (C) Transverse sections of an E12.5 Gata4H2B-GFP/+ embryo. GFP was visualised directly and DNA was stained with Hoechst. GFP expressing cells are present in the stomach epithelium and associated mesenchyme, the liver mesenchyme, the endocardium and myocardium of the heart, pancreas, midgut epithelium and the mesenchyme but not epithelium of the lung buds. Scale bars: 100 μm. Neural tube (NT), foregut (fg), hindgut (hg), right (R), left (L), dorsal (D), ventral (V), anterior (A), posterior (P).

At E12.5 GFP expression could be seen in the heart of Gata4H2B-GFP/+ embryos (Fig. 6B). Sections revealed high levels of GFP in the endocardium and myocardium of the heart (Fig. 6C). Tissue-specific expression of Gata4H2B-GFP was also detected in a number of gut, and gut-associated organs including the midgut endoderm, stomach epithelium and pancreatic bud (Fig. 6C), consistent with known sites of Gata4 expression (Ketola et al., 2004; Decker et al., 2006; Jacobsen et al., 2002; Heikinheimo et al., 1994). GFP expression was also present in the liver and in the lung bud mesenchyme, but absent in the endoderm-derived lung epithelium (Fig. 6C), as has been shown previously for Gata4 expression (Ackerman et al., 2007; Jay et al., 2007; Arceci et al., 1993).

Gata4H2B-GFP expression in adult mice

To assess the expression of the reporter in adult tissues, we visualised whole-mount organs for expression of GFP in both male and female mice at 3 months of age. In Gata4H2B-GFP/+ adults, GFP expression was observed in organs previously reported to express Gata4 including the heart, lung and liver as noted at E12.5, and in the adrenal gland (Fig. 7) (Arceci et al., 1993; Heikinheimo et al., 1994; Kiiveri et al., 2002). In addition, GFP expression was detected in the gonads, in both the ovary and testis, where Gata4 is required in granulosa cells for normal ovarian function, and in Sertoli cells for normal testicular function in both foetal and adult mice (Fig. 7) (Kyrönlahti et al., 2011b,a).

Fig. 7.
Fig. 7.

Gata4H2B-GFP/+ reporter expression in adult organs. Whole-mount immunofluorescence of Gata4H2B-GFP/+ organs from male and female adult mice at 3 months of age. GFP expressing organs include the ovaries, testes, heart, lung, adrenal gland, liver and gall bladder (gb). Right atrium (RA), right ventricle (RV), left ventricle (LV), left atrium (LA).

CONCLUSIONS

We have generated a novel Gata4H2B-GFP reporter and compared its pattern of expression to known sites of Gata4 mRNA expression and GATA4 protein expression domains. In contrast to previous transgenic reporters that are driven by enhancers or partial rat promoter sequences (Rojas et al., 2005, 2009, 2010; Mazaud Guittot et al., 2007; Pilon et al., 2008; Schachterle et al., 2012), this reporter which is targeted to the endogenous mouse locus retains all Gata4 cis-regulatory information.

Accordingly, this Gata4H2B-GFP transcriptional reporter faithfully recapitulated known embryonic Gata4 expression domains during embryonic development and in adult organs. In endodermal tissues, reporter expression labelled extra-embryonic endoderm and from mid-gestation stages the embryonic midgut endoderm, pancreas and stomach (Plusa et al., 2008; Kurimoto et al., 2006; Arceci et al., 1993; Cai et al., 2008; Heikinheimo et al., 1994; Morrisey et al., 1998; Ketola et al., 2004; Decker et al., 2006; Jacobsen et al., 2002). In mesodermal tissues, reporter expression labelled the cardiac lineage, the lateral plate mesoderm, septum transversum, and pulmonary and hepatic mesenchyme (Ackerman et al., 2007; Jay et al., 2007; Arceci et al., 1993; Nemer and Nemer, 2003; Rojas et al., 2005; Schachterle et al., 2012; Zhao et al., 2008; Heikinheimo et al., 1994). Transcriptional reporter expression strongly correlated with protein levels at pre-implantation embryonic stages, and GFP and GATA4 protein expression were largely co-expressed at post-implantation embryonic stages.

We demonstrated that at pre-implantation stages this Gata4H2B-GFP reporter is PrE-lineage specific. As Gata4 is not expressed in uncommitted ICM cells (Plusa et al., 2008; Artus et al., 2011; Kurimoto et al., 2006; Cai et al., 2008) we used it to visualise PrE emergence in live embryos. If combined with spectrally distinct Epi-specific reporter, Gata4H2B-GFP could be used to study initial specification events as the Epi and PrE lineages emerge from uncommitted ICM progenitors.

At post-implantation stages, Gata4H2B-GFP robustly labelled both embryonic and extra-embryonic VE cells. Primary defects in the VE cause Gata4−/− embryos to arrest between E7.5–E9.0 from either abnormal gastrulation, or later ventral closure defects (Molkentin et al., 1997; Kuo et al., 1997; Narita et al., 1997). Gata4 clearly plays an important role in VE morphogenesis, but this has not been well studied in vivo. Therefore, the Gata4H2B-GFP reporter allele will be a useful tool to study morphogenetic movements in the VE and interactions with the DE. Gata4H2B-GFP functions as a null allele so could be used to investigate perturbed functions of Gata4-deficient VE cells and probe the primary phenotype seen in Gata4−/− embryos (Molkentin et al., 1997; Kuo et al., 1997). In addition, the Gata4H2B-GFP allele can be Cre-excised to generate Gata4FLAG allele encoding an N-terminally FLAG-tagged GATA4 protein to study GATA4 transcriptional targets and co-factors in tissues of interest.

Another critical role for GATA4 is in cardiogenesis, and we demonstrate that the Gata4H2B-GFP labels the entire cardiac lineage from gastrulation stages into adulthood, recapitulating endogenous Gata4 expression (Auda-Boucher et al., 2000; Arceci et al., 1993; Heikinheimo et al., 1994; Schachterle et al., 2012; Rojas et al., 2005; Zhao et al., 2008). Hence, the Gata4H2B-GFP allele could be used for visualisation and/or FACS isolation of cardiac mesoderm cells at different embryonic stages.

In summary, Gata4H2B-GFP is a novel mouse line that permits the visualisation of Gata4 expressing cells at single-cell resolution due to the nuclear-localised fluorescent GFP reporter. This reporter was sufficiently bright to study tissues with time-lapse imaging and at single-cell resolution. As such, this Gata4H2B-GFP allele will be a useful tool for studying Gata4 expression and transcriptional regulation in a variety of contexts.

MATERIAL AND METHODS

Mice

The Gata4H2B-GFP/+ targeted allele was generated by inserting LoxP-H2B-GFP-4XPloyA-LoxP-FRT-Neo-FRT-3XFLAG cassette into the start codon of the Gata4 locus through homologous recombination. The targeting construct contained the cassette flanked by a 4.0kb 5′ homologous arm and a 4.0kb 3′ homologous arm in. The construct was linearised and electroporated into 129/SvJ mouse embryonic stem (ES) cells. Positive ES cells were identified by long-range PCR (Roche, Cat. 04829069001) with primer pair P1: 5′-TGGACGTGGACCACTGAGAGTAGG-3′ and P2: 5′-GCTTTAGTCACCGCCTTCTTGGAG-3′ and primer pair P3: 5′-CATGACATCGATTACAAGGATGACG-3′ and P4: 5′-CCTCAGTCTTCAACTCTCTGAACACC-3′. The PCR fragments were further verified by DNA sequencing. The targeted ES cells were microinjected into blastocysts to generate chimeric mice. The male F0 chimeric mice were crossed with Black Swiss females to generate F1 Gata4H2B-GFP/+ mice. The Neo cassette was removed by crossing with FLP recombinase mice (Rodríguez et al., 2000). To generate the Gata4FLAG mouse line, H2B-GFP was excised by crossing female Gata4H2B-GFP/+ mice with male Sox2.Cre mice (Hayashi et al., 2002). Gata4H2B-GFP/+ and Gata4FLAG/+ mice were then maintained on a CD1 outbred background. Gata4H2B-GFP mice were genotyped using standard GFP primers (Xenopoulos et al., 2015), and primers specific for the Gata4 wild-type allele (355 bp) P5: 5′-TTTCCTGAGCAAACCAGAGC-3′ and P6: 5′-CGGAGTGGGCACGTAGAC-3′. Gata4FLAG mice were genotyped using the primers P7: 5′-TTGAGCGAGTTGGGCCTCTCCTCG-3′ and P8: 5′-AGGGCCACCTGCTTCGTAGGCG-3′ to detect the FLAG (490 bp) and wild-type (230 bp) alleles.

Western blotting

Male Gata4FLAG/+ mice were mated with outbred CD1 female mice. Individual E7.5 embryo littermates were harvested for protein. Single embryos were lysed in 2% SDS and boiled at 95°C for 5 min. Protein extracts were applied for western blotting and detected with an anti-FLAG antibody (Sigma-Aldrich, F3165). Total protein lysate from a male Gata4FLAG/+ ear was used as positive control.

Embryo manipulation and culture

Gata4H2B-GFP/+ males were mated with outbred CD1 female mice. For pre-implantation embryo analysis E3.5–E4.5 blastocysts were flushed from uterine horns using flushing and holding medium (FHM, Millipore) as previously described (Behringer et al., 2014). The zona pellucida of the blastocysts was removed by incubation in acid Tyrode's solution (Sigma-Aldrich) at 37°C for 2 min. For live image analysis, blastocysts were moved into droplets of at least 1 μl of KSOM-AA (Millipore) culture medium per embryo or 10 μM cycloheximide (Sigma-Aldrich) in KSOM-AA, covered with mineral oil (Sigma-Aldrich) in 35 mm glass-bottomed dishes (MatTek). Cultured blastocysts were imaged overnight on a Zeiss LSM880 confocal microscope in a humidified incubation chamber with 5% CO2 at 37°C for 16 h and 12 h for cycloheximide treatments. Blastocysts were washed in PBS with 4 mg/ml BSA, prior to fixation in a solution of 4% PFA in PBS (BioRad) for 10 min at room temperature or overnight at 4°C, depending on the anti-GATA4 antibody used (see below). Fixed blastocysts were stored in PBS 4 mg/ml BSA until processing. For post-implantation embryos E5.5–E12.5 embryos were dissected from the maternal decidua in DMEM:F12 medium (Life Technologies). E5.5–E9.5 embryos were washed briefly in PBS and fixed in 4% PFA in PBS for 20 min at room temperature. E12.5 embryos were fixed at 1% PFA in PBS overnight at 4°C. Adult organs were fixed in 4% PFA in PBS for 2 h at room temperature.

Immunofluorescence of pre-implantation embryos

Pre-implantation embryo immunofluorescence was carried out as previously described (Saiz et al., 2016a). Briefly, fixed blastocysts were washed in PBX; 0.1% Triton X-100 (Sigma-Aldrich) in PBS, permeabilised for 5 min in a solution of 0.5% Triton X-100, 100 mM glycine in PBS and then washed in PBX for 5 min. Embryos were blocked in blocking buffer: 2% horse serum in PBS, for 40 min at room temperature, followed by incubation overnight at 4°C with primary antibodies diluted in blocking buffer (see antibody list below). The next day, embryos were washed three times in PBX (5 min each wash), incubated in blocking buffer for 40 min at room temperature before a 1 h incubation with secondary antibodies at 4°C. Embryos were then washed and incubated for at least 1 h in 5 μg/ml Hoechst in PBS.

For post-implantation stages, fixed embryos were washed in PBX, permeabilised in 0.5% Triton-X in PBS for 15 min and then washed three times in PBX. Embryos were then incubated in blocking buffer, for E5.5–E7.5; 2% Horse serum in PBX, and for >E8.5; 5% horse serum, 0.2% BSA, in PBX, for 2 h at room temperature, followed by incubation overnight at 4°C with primary antibodies diluted in blocking buffer. The next day, embryos were washed extensively in PBX, followed by incubation with secondary antibodies in blocking buffer for 2 h at room temperature, washed in PBX, and incubated with 5 μg/ml Hoechst in PBX to visualise DNA for 2 h to overnight prior to imaging.

Primary antibodies used were rabbit anti-GATA4 (Santa Cruz, sc-9053, 1:100), rabbit anti-NANOG (Reprocell, REC-RCAB0002PF, 1:500), goat anti-GATA4 (Santa Cruz, sc-1237, 1:100, samples fixed overnight at 4°C), goat anti-SOX17 (R&D Systems, AF1924, 1:100), goat anti-GATA6 (R&D Systems, AF1700, 1:100), goat anti-SOX7 (R&D Systems, AF2766, 1:100) and goat anti-BRACHYURY (R&D Systems, AF2085, 1:100). GFP was visualised directly except for when pre-implantation embryos were fixed overnight at 4°C (for use with goat anti-GATA4) when GFP was detected with chicken anti-GFP antibody (Aves, GFP1020, 1:400). Secondary antibodies used in this study were donkey anti-rabbit A647 (Life Technologies, A-315730), donkey anti-goat A568 (Life Technologies, A-11057), goat anti-rabbit A568 (Life Technologies, A-11011) and donkey anti-chicken A488 (Jackson Labs, 703-545-155) all used at 1:500 dilutions.

Cryo-sectioning of post-implantation embryos

Fixed embryos were washed in PBS and incubated in 10% sucrose for 30 min, followed by incubation in 30% sucrose for 4 h at room temperature. Embryos were then incubated in OCT (Tissue-Tek) overnight at 4°C and cryo-embedded in OCT by freezing on dry ice. Cryo-sections were cut at a 12 μm thickness on a Leica CM3050S cryostat. Cryo-sections that were not stained as whole-mount embryos were washed in PBS then counterstained by incubation in 5 μg/ml Hoechst in PBS for 30 min. Prior to mounting, sections were washed in PBS, and then in water, and mounted in Fluoromount G mounting media (Southern Biotech).

Image data acquisition

E3.5–E7.5 whole-mount embryos and cryo-sections were imaged on a Zeiss LSM880 laser scanning confocal microscope. Fixed whole-mount embryos were imaged in glass bottomed dishes (MatTek) in PBS (post-implantation) or 5 μg/ml Hoechst in PBS (pre-implantation). Fixed pre-implantation embryos were imaged along the entire z-axis with 1 μm z-steps using EC Plan-Neofluar 40×/1.30 oil immersion objective as described previously (Saiz et al., 2016a). Laser power was measured prior to each imaging session and laser output adjusted to maintain constant power across experiments for each laser line. Accordingly, imaging parameters for each antibody and laser line combination were the same across experiments, to reduce technical variability. For live imaging blastocysts were collected as described and imaged at 2 μm z-steps with a 15 min time-lapse interval. Whole mount embryos E8.5–E12.5 and organs from male and female 3-month-old adult mice were imaged whole-mount on a Leica M165 FC fluorescence stereomicroscope. Raw images were processed in Image J. Time-lapse movies were compiled in Imaris and Quicktime.

Calculation of half-life in cycloheximide treated embryos

Individual Gata4H2B-GFP positive PrE cells in time-lapse movies of control (KSOM) and cycloheximide treated embryos were manually tracked in Imaris. High concentrations of cycloheximide are toxic for embryos, therefore we used a relatively low concentration (10 μM) to partially inhibit protein translation, although we still observed increased rates of cell death in cyclohexmide treated embryos. To ensure we only measured H2B-GFP decay, and not the emergence of Gata4 expressing PrE cells, we only tracked GFP positive cells present at the beginning of the time-lapse movies (∼E4.0). Noise in the raw GFP nuclear fluorescence intensity due to decay in fluorescent signal along the z-axis was not corrected for. In addition, only a few GFP+ cells in cycloheximide treated embryos could be tracked throughout the entire 12 h movie. Shortened tracks were either due to the signal decaying to background levels so that the cell could no longer be identified within the ICM, or apoptosis at earlier time points. Therefore, to normalise for intensity changes within each cell, an exponential decay model for GFP was fitted for individual cell tracks over time. Exponential decay was fitted using Eqn 1. [GFP]t is the fluorescent intensity of GFP at time=t, [GFP]0 is the initial fluorescence intensity of GFP, t is time in hours, and k is the decay constant which can also be expressed as the rate of change in log[GFP] as plotted in Fig. S2B. Using Eqn 2 we calculated the half-life (t1/2) of H2B-GFP for each individually tracked cell. For values where k→0 then t1/2→∞ and skew the mean of t1/2, therefore we calculated median as a better measure of central tendency, and the median t1/2=5.5 h. Embedded Image(1) Embedded Image(2)

Quantitative analysis of pre-implantation embryos

Semi-automated 3D nuclear segmentation for cell count staging and quantification of nuclear fluorescence intensity was carried out using MINS, a nuclear-segmentation algorithm, as described previously (Saiz et al., 2016a; Lou et al., 2014). Correction for fluorescence decay along the z-axis was performed by fitting linear regressions to the logarithm of fluorescence values as a function of the z-axis and correcting using an empirical Bayes method, as detailed in (Saiz et al., 2016b). All analysis of fluorescence intensity levels and statistics were performed using R (http://www.r-project.org/). Lineage assignments were determined as follows. Trophectoderm cells were manually scored based on position and morphology. ICM cells were identified as GATA4+PrE or GATA4−ICM cells based on threshold levels of GATA4 protein manually determined from frequency density plots (Fig. S1A). Mitotic cells were excluded from statistical analysis of correlation between GATA4 and GFP fluorescent intensity levels, as H2B-GFP remains bound to chromatin in the absence of nuclear GATA4 protein (Fig. 2B). ICM cell identities were assigned based on relative GATA6 and NANOG levels using a k-means clustering approach as previously described (Saiz et al., 2016b,a), as PrE (GATA6+), EPI (NANOG+), double positive (DP, NANOG+GATA6+) or double negative (DN, NANOG−GATA6−) (Fig. 2C, Fig. S1C).

Primitive endoderm identity was assigned as ICM cells exceeding a manually determined threshold level of GATA4 protein based on frequency density plots of expression levels TE (negative) versus ICM (positive and negative, bimodal). The remaining unassigned ICM cells were classified as ‘epiblast/uncommitted ICM’ as these possibilities could not be distinguished in this experimental regime. Mitotic cells were excluded from statistical analysis of fluorescent intensity levels, as H2B-GFP remains bound to chromatin in the absence of nuclear GATA4 protein.

Acknowledgements

We thank the mouse facility at Mount Sinai Hospital, New York for the generation of the germline transmitting chimeras. We thank members of the Hadjantonakis lab for helpful feedback on the characterisation of this report, and Sophie Morgani for comments on the manuscript.

Footnotes

  • Competing interests

    The authors declare no competing or financial interests.

  • Author contributions

    Conceptualization: C.S.S., A.-K.H., C.-L.C.; Methodology: C.S.S., L.Z., T.W., W.C., N.S., S.N.; Validation: C.S.S., L.Z., T.W., W.C., N.S., S.N.; Formal analysis: C.S.; Investigation: C.S.S.; Resources: C.S.S., L.Z., T.W., W.C., S.N.; Data curation: C.S.S.; Writing - original draft: C.S.S.; Writing - review & editing: C.S.S., A.-K.H., C.-L.C.; Visualization: C.S.S.; Supervision: A.-K.H., C.-L.C.; Project administration: A.-K.H., C.-L.C.; Funding acquisition: A.-K.H., C.-L.C.

  • Funding

    This work was supported by grants from the National Institutes of Health (NIH) to A.-K.H. (R01DK084391, R01HD094868 and P30CA008748) and C.-L.C. (1R01HL131735 and 1R01HL095810).

  • Supplementary information

    Supplementary information available online at http://bio.biologists.org/lookup/doi/10.1242/bio.036517.supplemental

  • Received June 14, 2018.
  • Accepted October 22, 2018.
http://creativecommons.org/licenses/by/4.0

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

References

  1. ↵
    1. Ackerman, K. G.,
    2. Wang, J.,
    3. Luo, L.,
    4. Fujiwara, Y.,
    5. Orkin, S. H. and
    6. Beier, D. R.
    (2007). Gata4 is necessary for normal pulmonary lobar development. Am. J. Respir. Cell Mol. Biol. 36, 391-397. doi:10.1165/rcmb.2006-0211RC
    OpenUrlCrossRefPubMedWeb of Science
  2. ↵
    1. Arceci, R. J.,
    2. King, A. A.,
    3. Simon, M. C.,
    4. Orkin, S. H. and
    5. Wilson, D. B.
    (1993). Mouse GATA-4: a retinoic acid-inducible GATA-binding transcription factor expressed in endodermally derived tissues and heart. Mol. Cell. Biol. 13, 2235-2246. doi:10.1128/MCB.13.4.2235
    OpenUrlAbstract/FREE Full Text
  3. ↵
    1. Arnold, S. J. and
    2. Robertson, E. J.
    (2009). Making a commitment: cell lineage allocation and axis patterning in the early mouse embryo. Nat. Rev. Mol. Cell Biol. 10, 91-103. doi:10.1038/nrm2618
    OpenUrlCrossRefPubMedWeb of Science
  4. ↵
    1. Artus, J.,
    2. Piliszek, A. and
    3. Hadjantonakis, A.-K.
    (2011). The primitive endoderm lineage of the mouse blastocyst: sequential transcription factor activation and regulation of differentiation by Sox17. Dev. Biol. 350, 393-404. doi:10.1016/j.ydbio.2010.12.007
    OpenUrlCrossRefPubMed
  5. ↵
    1. Auda-Boucher, G.,
    2. Bernard, B.,
    3. Fontaine-Pérus, J.,
    4. Rouaud, T.,
    5. Mericksay, M. and
    6. Gardahaut, M.-F.
    (2000). Staging of the commitment of murine cardiac cell progenitors. Dev. Biol. 225, 214-225. doi:10.1006/dbio.2000.9817
    OpenUrlCrossRefPubMedWeb of Science
  6. ↵
    1. Behringer, R.,
    2. Gertsenstein, M.,
    3. Nagy, K. V. and
    4. Nagy, A.
    (2014). Manipulating the Mouse Embryo: a Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
  7. ↵
    1. Borok, M. J.,
    2. Papaioannou, V. E. and
    3. Sussel, L.
    (2016). Unique functions of Gata4 in mouse liver induction and heart development. Dev. Biol. 410, 213-222. doi:10.1016/j.ydbio.2015.12.007
    OpenUrlCrossRef
  8. ↵
    1. Cai, K. Q.,
    2. Capo-Chichi, C. D.,
    3. Rula, M. E.,
    4. Yang, D.-H. and
    5. Xu, X.-X.
    (2008). Dynamic GATA6 expression in primitive endoderm formation and maturation in early mouse embryogenesis. Dev. Dyn. 237, 2820-2829. doi:10.1002/dvdy.21703
    OpenUrlCrossRefPubMed
  9. ↵
    1. Chazaud, C. and
    2. Yamanaka, Y.
    (2016). Lineage specification in the mouse preimplantation embryo. Development 143, 1063-1074. doi:10.1242/dev.128314
    OpenUrlAbstract/FREE Full Text
  10. ↵
    1. Chazaud, C.,
    2. Yamanaka, Y.,
    3. Pawson, T. and
    4. Rossant, J.
    (2006). Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway. Dev. Cell 10, 615-624. doi:10.1016/j.devcel.2006.02.020
    OpenUrlCrossRefPubMedWeb of Science
  11. ↵
    1. Crispino, J. D. and
    2. Horwitz, M. S.
    (2017). GATA factor mutations in hematologic disease. Blood 129, 2103-2110. doi:10.1182/blood-2016-09-687889
    OpenUrlAbstract/FREE Full Text
  12. ↵
    1. Crispino, J. D.,
    2. Lodish, M. B.,
    3. Thurberg, B. L.,
    4. Litovsky, S. H.,
    5. Collins, T.,
    6. Molkentin, J. D. and
    7. Orkin, S. H.
    (2001). Proper coronary vascular development and heart morphogenesis depend on interaction of GATA-4 with FOG cofactors. Genes Dev. 15, 839-844. doi:10.1101/gad.875201
    OpenUrlAbstract/FREE Full Text
  13. ↵
    1. Decker, K.,
    2. Goldman, D. C.,
    3. Grasch, C. L. and
    4. Sussel, L.
    (2006). Gata6 is an important regulator of mouse pancreas development. Dev. Biol. 298, 415-429. doi:10.1016/j.ydbio.2006.06.046
    OpenUrlCrossRefPubMedWeb of Science
  14. ↵
    1. Freyer, L.,
    2. Schröter, C.,
    3. Saiz, N.,
    4. Schrode, N.,
    5. Nowotschin, S.,
    6. Martinez-Arias, A. and
    7. Hadjantonakis, A.-K.
    (2015). A loss-of-function and H2B-Venus transcriptional reporter allele for Gata6 in mice. BMC Dev. Biol. 15, 38. doi:10.1186/s12861-015-0086-5
    OpenUrlCrossRefPubMed
  15. ↵
    1. Fujikura, J.,
    2. Yamato, E.,
    3. Yonemura, S.,
    4. Hosoda, K.,
    5. Masui, S.,
    6. Nakao, K.,
    7. Miyazaki Ji, J. and
    8. Niwa, H.
    (2002). Differentiation of embryonic stem cells is induced by GATA factors. Genes Dev. 16, 784-789. doi:10.1101/gad.968802
    OpenUrlAbstract/FREE Full Text
  16. ↵
    1. Garg, V.,
    2. Kathiriya, I. S.,
    3. Barnes, R.,
    4. Schluterman, M. K.,
    5. King, I. N.,
    6. Butler, C. A.,
    7. Rothrock, C. R.,
    8. Eapen, R. S.,
    9. Hirayama-Yamada, K.,
    10. Joo, K. et al.
    (2003). GATA4 mutations cause human congenital heart defects and reveal an interaction with TBX5. Nature 424, 443-447. doi:10.1038/nature01827
    OpenUrlCrossRefPubMedWeb of Science
  17. ↵
    1. Hadjantonakis, A.-K. and
    2. Papaioannou, V. E.
    (2004). Dynamic in vivo imaging and cell tracking using a histone fluorescent protein fusion in mice. BMC Biotechnol. 4, 33. doi:10.1186/1472-6750-4-33
    OpenUrlCrossRefPubMed
  18. ↵
    1. Hayashi, S.,
    2. Lewis, P.,
    3. Pevny, L. and
    4. Mcmahon, A. P.
    (2002). Efficient gene modulation in mouse epiblast using a Sox2Cre transgenic mouse strain. Mech. Dev. 119, S97-S101. doi:10.1016/S0925-4773(03)00099-6
    OpenUrlCrossRefPubMedWeb of Science
  19. ↵
    1. Heikinheimo, M.,
    2. Scandrett, J. M. and
    3. Wilson, D. B.
    (1994). Localization of transcription factor GATA-4 to regions of the mouse embryo involved in cardiac development. Dev. Biol. 164, 361-373. doi:10.1006/dbio.1994.1206
    OpenUrlCrossRefPubMedWeb of Science
  20. ↵
    1. Jacobsen, C. M.,
    2. Narita, N.,
    3. Bielinska, M.,
    4. Syder, A. J.,
    5. Gordon, J. I. and
    6. Wilson, D. B.
    (2002). Genetic mosaic analysis reveals that GATA-4 is required for proper differentiation of mouse gastric epithelium. Dev. Biol. 241, 34-46. doi:10.1006/dbio.2001.0424
    OpenUrlCrossRefPubMedWeb of Science
  21. ↵
    1. Jay, P. Y.,
    2. Bielinska, M.,
    3. Erlich, J. M.,
    4. Mannisto, S.,
    5. Pu, W. T.,
    6. Heikinheimo, M. and
    7. Wilson, D. B.
    (2007). Impaired mesenchymal cell function in Gata4 mutant mice leads to diaphragmatic hernias and primary lung defects. Dev. Biol. 301, 602-614. doi:10.1016/j.ydbio.2006.09.050
    OpenUrlCrossRefPubMedWeb of Science
  22. ↵
    1. Kanda, T.,
    2. Sullivan, K. F. and
    3. Wahl, G. M.
    (1998). Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr. Biol. 8, 377-385. doi:10.1016/S0960-9822(98)70156-3
    OpenUrlCrossRefPubMedWeb of Science
  23. ↵
    1. Ketola, I.,
    2. Otonkoski, T.,
    3. Pulkkinen, M.-A.,
    4. Niemi, H.,
    5. Palgi, J.,
    6. Jacobsen, C. M.,
    7. Wilson, D. B. and
    8. Heikinheimo, M.
    (2004). Transcription factor GATA-6 is expressed in the endocrine and GATA-4 in the exocrine pancreas. Mol. Cell. Endocrinol. 226, 51-57. doi:10.1016/j.mce.2004.06.007
    OpenUrlCrossRefPubMed
  24. ↵
    1. Kiiveri, S.,
    2. Liu, J.,
    3. Westerholm-Ormio, M.,
    4. Narita, N.,
    5. Wilson, D. B.,
    6. Voutilainen, R. and
    7. Heikinheimo, M
    . (2002). Differential expression of GATA-4 and GATA-6 in fetal and adult mouse and human adrenal tissue. Endocrinology 143, 3136-3143. doi:10.1210/endo.143.8.8939
    OpenUrlCrossRefPubMedWeb of Science
  25. ↵
    1. Kuo, C. T.,
    2. Morrisey, E. E.,
    3. Anandappa, R.,
    4. Sigrist, K.,
    5. Lu, M. M.,
    6. Parmacek, M. S.,
    7. Soudais, C. and
    8. Leiden, J. M.
    (1997). GATA4 transcription factor is required for ventral morphogenesis and heart tube formation. Genes Dev. 11, 1048-1060. doi:10.1101/gad.11.8.1048
    OpenUrlAbstract/FREE Full Text
  26. ↵
    1. Kurimoto, K.,
    2. Yabuta, Y.,
    3. Ohinata, Y.,
    4. Ono, Y.,
    5. Uno, K. D.,
    6. Yamada, R. G.,
    7. Ueda, H. R. and
    8. Saitou, M.
    (2006). An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis. Nucleic Acids Res. 34, e42. doi:10.1093/nar/gkl050
    OpenUrlCrossRefPubMed
  27. ↵
    1. Kwon, G. S.,
    2. Viotti, M. and
    3. Hadjantonakis, A.-K.
    (2008). The endoderm of the mouse embryo arises by dynamic widespread intercalation of embryonic and extraembryonic lineages. Dev. Cell 15, 509-520. doi:10.1016/j.devcel.2008.07.017
    OpenUrlCrossRefPubMedWeb of Science
  28. ↵
    1. Kyrönlahti, A.,
    2. Euler, R.,
    3. Bielinska, M.,
    4. Schoeller, E. L.,
    5. Moley, K. H.,
    6. Toppari, J.,
    7. Heikinheimo, M. and
    8. Wilson, D. B.
    (2011a). GATA4 regulates Sertoli cell function and fertility in adult male mice. Mol. Cell. Endocrinol. 333, 85-95. doi:10.1016/j.mce.2010.12.019
    OpenUrlCrossRefPubMed
  29. ↵
    1. Kyrönlahti, A.,
    2. Vetter, M.,
    3. Euler, R.,
    4. Bielinska, M.,
    5. Jay, P. Y.,
    6. Anttonen, M.,
    7. Heikinheimo, M. and
    8. Wilson, D. B.
    (2011b). GATA4 deficiency impairs ovarian function in adult mice. Biol. Reprod. 84, 1033-1044. doi:10.1095/biolreprod.110.086850
    OpenUrlAbstract/FREE Full Text
  30. ↵
    1. Lentjes, M. H. F. M.,
    2. Niessen, H. E. C.,
    3. Akiyama, Y.,
    4. De Bruïne, A. P.,
    5. Melotte, V. and
    6. Van Engeland, M.
    (2016). The emerging role of GATA transcription factors in development and disease. Expert Rev. Mol. Med. 18, e3. doi:10.1017/erm.2016.2
    OpenUrlCrossRefPubMed
  31. ↵
    1. Lou, X.,
    2. Kang, M.,
    3. Xenopoulos, P.,
    4. Muñoz-Descalzo, S. and
    5. Hadjantonakis, A.-K.
    (2014). A rapid and efficient 2D/3D nuclear segmentation method for analysis of early mouse embryo and stem cell image data. Stem Cell Reports 2, 382-397. doi:10.1016/j.stemcr.2014.01.010
    OpenUrlCrossRef
  32. ↵
    1. Lourenco, D.,
    2. Brauner, R.,
    3. Rybczynska, M.,
    4. Nihoul-Fekete, C.,
    5. Mcelreavey, K. and
    6. Bashamboo, A.
    (2011). Loss-of-function mutation in GATA4 causes anomalies of human testicular development. Proc. Natl. Acad. Sci. USA 108, 1597-1602. doi:10.1073/pnas.1010257108
    OpenUrlAbstract/FREE Full Text
  33. ↵
    1. Mazaud Guittot, S.,
    2. Tétu, A.,
    3. Legault, E.,
    4. Pilon, N.,
    5. Silversides, D. W. and
    6. Viger, R. S.
    (2007). The proximal Gata4 promoter directs reporter gene expression to sertoli cells during mouse gonadal development. Biol. Reprod. 76, 85-95. doi:10.1095/biolreprod.106.055137
    OpenUrlAbstract/FREE Full Text
  34. ↵
    1. Merika, M. and
    2. Orkin, S. H.
    (1993). DNA-binding specificity of GATA family transcription factors. Mol. Cell. Biol. 13, 3999-4010. doi:10.1128/MCB.13.7.3999
    OpenUrlAbstract/FREE Full Text
  35. ↵
    1. Molkentin, J. D.,
    2. Lin, Q.,
    3. Duncan, S. A. and
    4. Olson, E. N.
    (1997). Requirement of the transcription factor GATA4 for heart tube formation and ventral morphogenesis. Genes Dev. 11, 1061-1072. doi:10.1101/gad.11.8.1061
    OpenUrlAbstract/FREE Full Text
  36. ↵
    1. Morris, S. A.,
    2. Teo, R. T. Y.,
    3. Li, H.,
    4. Robson, P.,
    5. Glover, D. M. and
    6. Zernicka-Goetz, M.
    (2010). Origin and formation of the first two distinct cell types of the inner cell mass in the mouse embryo. Proc. Natl. Acad. Sci. USA 107, 6364-6369. doi:10.1073/pnas.0915063107
    OpenUrlAbstract/FREE Full Text
  37. ↵
    1. Morrisey, E. E.,
    2. Ip, H. S.,
    3. Lu, M. M. and
    4. Parmacek, M. S.
    (1996). GATA-6: a zinc finger transcription factor that is expressed in multiple cell lineages derived from lateral mesoderm. Dev. Biol. 177, 309-322. doi:10.1006/dbio.1996.0165
    OpenUrlCrossRefPubMedWeb of Science
  38. ↵
    1. Morrisey, E. E.,
    2. Tang, Z.,
    3. Sigrist, K.,
    4. Lu, M. M.,
    5. Jiang, F.,
    6. Ip, H. S. and
    7. Parmacek, M. S.
    (1998). GATA6 regulates HNF4 and is required for differentiation of visceral endoderm in the mouse embryo. Genes Dev. 12, 3579-3590. doi:10.1101/gad.12.22.3579
    OpenUrlAbstract/FREE Full Text
  39. ↵
    1. Narita, N.,
    2. Bielinska, M. and
    3. Wilson, D. B.
    (1997). Wild-type endoderm abrogates the ventral developmental defects associated with GATA-4 deficiency in the mouse. Dev. Biol. 189, 270-274. doi:10.1006/dbio.1997.8684
    OpenUrlCrossRefPubMedWeb of Science
  40. ↵
    1. Nemer, G. and
    2. Nemer, M.
    (2003). Transcriptional activation of BMP-4 and regulation of mammalian organogenesis by GATA-4 and −6. Dev. Biol. 254, 131-148. doi:10.1016/S0012-1606(02)00026-X
    OpenUrlCrossRefPubMedWeb of Science
  41. ↵
    1. Niakan, K. K.,
    2. Ji, H.,
    3. Maehr, R.,
    4. Vokes, S. A.,
    5. Rodolfa, K. T.,
    6. Sherwood, R. I.,
    7. Yamaki, M.,
    8. Dimos, J. T.,
    9. Chen, A. E.,
    10. Melton, D. A. et al.
    (2010). Sox17 promotes differentiation in mouse embryonic stem cells by directly regulating extraembryonic gene expression and indirectly antagonizing self-renewal. Genes Dev. 24, 312-326. doi:10.1101/gad.1833510
    OpenUrlAbstract/FREE Full Text
  42. ↵
    1. Niakan, K. K.,
    2. Schrode, N.,
    3. Cho, L. T. Y. and
    4. Hadjantonakis, A.-K.
    (2013). Derivation of extraembryonic endoderm stem (XEN) cells from mouse embryos and embryonic stem cells. Nat. Protoc. 8, 1028-1041. doi:10.1038/nprot.2013.049
    OpenUrlCrossRefPubMed
  43. ↵
    1. Ohnishi, Y.,
    2. Huber, W.,
    3. Tsumura, A.,
    4. Kang, M.,
    5. Xenopoulos, P.,
    6. Kurimoto, K.,
    7. Oleś, A. K.,
    8. Araúzo-Bravo, M. J.,
    9. Saitou, M.,
    10. Hadjantonakis, A.-K. et al.
    (2014). Cell-to-cell expression variability followed by signal reinforcement progressively segregates early mouse lineages. Nat. Cell Biol. 16, 27-37. doi:10.1038/ncb2881
    OpenUrlCrossRefPubMedWeb of Science
  44. ↵
    1. Oka, T.,
    2. Maillet, M.,
    3. Watt, A. J.,
    4. Schwartz, R. J.,
    5. Aronow, B. J.,
    6. Duncan, S. A. and
    7. Molkentin, J. D.
    (2006). Cardiac-specific deletion of Gata4 reveals its requirement for hypertrophy, compensation, and myocyte viability. Circ. Res. 98, 837-845. doi:10.1161/01.RES.0000215985.18538.c4
    OpenUrlAbstract/FREE Full Text
  45. ↵
    1. Pehlivan, T.,
    2. Pober, B. R.,
    3. Brueckner, M.,
    4. Garrett, S.,
    5. Slaugh, R.,
    6. Van Rheeden, R.,
    7. Wilson, D. B.,
    8. Watson, M. S. and
    9. Hing, A. V.
    (1999). GATA4 haploinsufficiency in patients with interstitial deletion of chromosome region 8p23.1 and congenital heart disease. Am. J. Med. Genet. 83, 201-206. doi:10.1002/(SICI)1096-8628(19990319)83:3<201::AID-AJMG11>3.0.CO;2-V
    OpenUrlCrossRefPubMedWeb of Science
  46. ↵
    1. Peterkin, T.,
    2. Gibson, A.,
    3. Loose, M. and
    4. Patient, R.
    (2005). The roles of GATA-4, −5 and −6 in vertebrate heart development. Semin. Cell Dev. Biol. 16, 83-94. doi:10.1016/j.semcdb.2004.10.003
    OpenUrlCrossRefPubMedWeb of Science
  47. ↵
    1. Pikkarainen, S.,
    2. Tokola, H.,
    3. Kerkela, R. and
    4. Ruskoaho, H.
    (2004). GATA transcription factors in the developing and adult heart. Cardiovasc. Res. 63, 196-207. doi:10.1016/j.cardiores.2004.03.025
    OpenUrlCrossRefPubMedWeb of Science
  48. ↵
    1. Pilon, N.,
    2. Raiwet, D.,
    3. Viger, R. S. and
    4. Silversides, D. W.
    (2008). Novel pre- and post-gastrulation expression of Gata4 within cells of the inner cell mass and migratory neural crest cells. Dev. Dyn. 237, 1133-1143. doi:10.1002/dvdy.21496
    OpenUrlCrossRefPubMedWeb of Science
  49. ↵
    1. Plusa, B.,
    2. Piliszek, A.,
    3. Frankenberg, S.,
    4. Artus, J. and
    5. Hadjantonakis, A.-K.
    (2008). Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Development 135, 3081-3091. doi:10.1242/dev.021519
    OpenUrlAbstract/FREE Full Text
  50. ↵
    1. Rivera-Feliciano, J.,
    2. Lee, K. H.,
    3. Kong, S. W.,
    4. Rajagopal, S.,
    5. Ma, Q.,
    6. Springer, Z.,
    7. Izumo, S.,
    8. Tabin, C. J. and
    9. Pu, W. T.
    (2006). Development of heart valves requires Gata4 expression in endothelial-derived cells. Development 133, 3607-3618. doi:10.1242/dev.02519
    OpenUrlAbstract/FREE Full Text
  51. ↵
    1. Rodríguez, C. I.,
    2. Buchholz, F.,
    3. Galloway, J.,
    4. Sequerra, R.,
    5. Kasper, J.,
    6. Ayala, R.,
    7. Stewart, A. F. and
    8. Dymecki, S. M.
    (2000). High-efficiency deleter mice show that FLPe is an alternative to Cre-loxP. Nat. Genet. 25, 139-140. doi:10.1038/75973
    OpenUrlCrossRefPubMedWeb of Science
  52. ↵
    1. Rojas, A.,
    2. De Val, S.,
    3. Heidt, A. B.,
    4. Xu, S. M.,
    5. Bristow, J. and
    6. Black, B. L.
    (2005). Gata4 expression in lateral mesoderm is downstream of BMP4 and is activated directly by Forkhead and GATA transcription factors through a distal enhancer element. Development 132, 3405-3417. doi:10.1242/dev.01913
    OpenUrlAbstract/FREE Full Text
  53. ↵
    1. Rojas, A.,
    2. Kong, S. W.,
    3. Agarwal, P.,
    4. Gilliss, B.,
    5. Pu, W. T. and
    6. Black, B. L.
    (2008). GATA4 is a direct transcriptional activator of cyclin D2 and Cdk4 and is required for cardiomyocyte proliferation in anterior heart field-derived myocardium. Mol. Cell. Biol. 28, 5420-5431. doi:10.1128/MCB.00717-08
    OpenUrlAbstract/FREE Full Text
  54. ↵
    1. Rojas, A.,
    2. Schachterle, W.,
    3. Xu, S.-M. and
    4. Black, B. L.
    (2009). An endoderm-specific transcriptional enhancer from the mouse Gata4 gene requires GATA and homeodomain protein-binding sites for function in vivo. Dev. Dyn. 238, 2588-2598. doi:10.1002/dvdy.22091
    OpenUrlCrossRefPubMed
  55. ↵
    1. Rojas, A.,
    2. Schachterle, W.,
    3. Xu, S.-M.,
    4. Martín, F. and
    5. Black, B. L.
    (2010). Direct transcriptional regulation of Gata4 during early endoderm specification is controlled by FoxA2 binding to an intronic enhancer. Dev. Biol. 346, 346-355. doi:10.1016/j.ydbio.2010.07.032
    OpenUrlCrossRefPubMed
  56. ↵
    1. Rossant, J.
    (2016). Making the mouse blastocyst: past, present, and future. Curr. Top. Dev. Biol. 117, 275-288. doi:10.1016/bs.ctdb.2015.11.015
    OpenUrlCrossRef
  57. ↵
    1. Saiz, N.,
    2. Kang, M.,
    3. Schrode, N.,
    4. Lou, X. and
    5. Hadjantonakis, A. K.
    (2016a). Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos. J. Vis. Exp. 108, e53654. doi:10.3791/53654
    OpenUrlCrossRef
  58. ↵
    1. Saiz, N.,
    2. Williams, K. M.,
    3. Seshan, V. E. and
    4. Hadjantonakis, A.-K.
    (2016b). Asynchronous fate decisions by single cells collectively ensure consistent lineage composition in the mouse blastocyst. Nat. Commun. 7, 13463. doi:10.1038/ncomms13463
    OpenUrlCrossRef
  59. ↵
    1. Schachterle, W.,
    2. Rojas, A.,
    3. Xu, S.-M. and
    4. Black, B. L.
    (2012). ETS-dependent regulation of a distal Gata4 cardiac enhancer. Dev. Biol. 361, 439-449. doi:10.1016/j.ydbio.2011.10.023
    OpenUrlCrossRefPubMed
  60. ↵
    1. Schroter, C.,
    2. Rue, P.,
    3. Mackenzie, J. P. and
    4. Martinez Arias, A.
    (2015). FGF/MAPK signaling sets the switching threshold of a bistable circuit controlling cell fate decisions in embryonic stem cells. Development 142, 4205-4216. doi:10.1242/dev.127530
    OpenUrlAbstract/FREE Full Text
  61. ↵
    1. Shimosato, D.,
    2. Shiki, M. and
    3. Niwa, H.
    (2007). Extra-embryonic endoderm cells derived from ES cells induced by GATA factors acquire the character of XEN cells. BMC Dev. Biol. 7, 80. doi:10.1186/1471-213X-7-80
    OpenUrlCrossRefPubMed
  62. ↵
    1. Soudais, C.,
    2. Bielinska, M.,
    3. Heikinheimo, M.,
    4. Macarthur, C. A.,
    5. Narita, N.,
    6. Saffitz, J. E.,
    7. Simon, M. C.,
    8. Leiden, J. M. and
    9. Wilson, D. B.
    (1995). Targeted mutagenesis of the transcription factor GATA-4 gene in mouse embryonic stem cells disrupts visceral endoderm differentiation in vitro. Development 121, 3877-3888.
    OpenUrlAbstract
  63. ↵
    1. Viger, R. S.,
    2. Guittot, S. M.,
    3. Anttonen, M.,
    4. Wilson, D. B. and
    5. Heikinheimo, M.
    (2008). Role of the GATA family of transcription factors in endocrine development, function, and disease. Mol. Endocrinol. 22, 781-798. doi:10.1210/me.2007-0513
    OpenUrlCrossRefPubMedWeb of Science
  64. ↵
    1. Viotti, M.,
    2. Foley, A. C. and
    3. Hadjantonakis, A.-K.
    (2014a). Gutsy moves in mice: cellular and molecular dynamics of endoderm morphogenesis. Phil. Trans. R. Soc. B 369, 20130547. doi:10.1098/rstb.2013.0547
    OpenUrlCrossRefPubMed
  65. ↵
    1. Viotti, M.,
    2. Nowotschin, S. and
    3. Hadjantonakis, A.-K.
    (2014b). SOX17 links gut endoderm morphogenesis and germ layer segregation. Nat. Cell Biol. 16, 1146-1156. doi:10.1038/ncb3070
    OpenUrlCrossRefPubMed
  66. ↵
    1. Wang, J.,
    2. Fang, M.,
    3. Liu, X. Y.,
    4. Xin, Y. F.,
    5. Liu, Z. M.,
    6. Chen, X. Z.,
    7. Wang, X. Z.,
    8. Fang, W. Y.,
    9. Liu, X. and
    10. Yang, Y. Q.
    (2011). A novel GATA4 mutation responsible for congenital ventricular septal defects. Int. J. Mol. Med. 28, 557-564.
    OpenUrlPubMed
  67. ↵
    1. Watt, A. J.,
    2. Battle, M. A.,
    3. Li, J. and
    4. Duncan, S. A.
    (2004). GATA4 is essential for formation of the proepicardium and regulates cardiogenesis. Proc. Natl. Acad. Sci. USA 101, 12573-12578. doi:10.1073/pnas.0400752101
    OpenUrlAbstract/FREE Full Text
  68. ↵
    1. Watt, A. J.,
    2. Zhao, R.,
    3. Li, J. and
    4. Duncan, S. A.
    (2007). Development of the mammalian liver and ventral pancreas is dependent on GATA4. BMC Dev. Biol. 7, 37. doi:10.1186/1471-213X-7-37
    OpenUrlCrossRefPubMed
  69. ↵
    1. Xenopoulos, P.,
    2. Kang, M.,
    3. Puliafito, A.,
    4. Di Talia, S. and
    5. Hadjantonakis, A.-K.
    (2015). Heterogeneities in nanog expression drive stable commitment to pluripotency in the mouse blastocyst. Cell Rep 10, 1508-01520. doi:10.1016/j.celrep.2015.02.010
    OpenUrlCrossRef
  70. ↵
    1. Zeisberg, E. M.,
    2. Ma, Q.,
    3. Juraszek, A. L.,
    4. Moses, K.,
    5. Schwartz, R. J.,
    6. Izumo, S. and
    7. Pu, W. T.
    (2005). Morphogenesis of the right ventricle requires myocardial expression of Gata4. J. Clin. Invest. 115, 1522-1531. doi:10.1172/JCI23769
    OpenUrlCrossRefPubMedWeb of Science
  71. ↵
    1. Zhao, R.,
    2. Watt, A. J.,
    3. Battle, M. A.,
    4. Li, J.,
    5. Bondow, B. J. and
    6. Duncan, S. A.
    (2008). Loss of both GATA4 and GATA6 blocks cardiac myocyte differentiation and results in acardia in mice. Dev. Biol. 317, 614-619. doi:10.1016/j.ydbio.2008.03.013
    OpenUrlCrossRefPubMedWeb of Science
View Abstract
Back to top

RSSRSS

Keywords

 Download PDF

Email
Research Article
A Gata4 nuclear GFP transcriptional reporter to study endoderm and cardiac development in the mouse
Claire S. Simon, Lu Zhang, Tao Wu, Weibin Cai, Nestor Saiz, Sonja Nowotschin, Chen-Leng Cai, Anna-Katerina Hadjantonakis
Biology Open 2018 7: bio036517 doi: 10.1242/bio.036517 Published 10 December 2018
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Alerts

Article Navigation

Other journals from The Company of Biologists

Advertisement

First person interviews — Kim, Isabel and Antonio

Kim, Isabel and Antonio

In the latest interviews with early-career authors of our papers, Antonio Barral, Isabel Rollan and Kim Nguyen discuss their scientific backgrounds, research and future directions.

We're excited to be an affiliate journal for Review Commons, the ASAPbio/EMBO platform for high-quality journal-independent peer-review in the life sciences, which went live on 09 December.

On the cover of issue 11, Tawari, Wodarz et al. identify CG4038 as a gene downregulated in bam mutant ovaries in an RNA-seq transcriptome analysis.

Dates for 2020 — Grants, Meetings and Workshops

Get ahead and update your 2020 diary with our meeting grant deadlines! The Company of Biologists also has a fantastic line-up of Workshops with funded places for early-career researchers and larger Journal Meetings from our sister journals Development, Journal of Cell Science, Journal of Experimental Biology and Disease, Models & Mechanisms.

Why publish with BiO?

Biology Open aims to provide rapid publication for scientifically sound observations and valid conclusions in developmental, cell, experimental and translational biology. Submit your paper for an easy and fast publication process. Recent highlights in BiO by Maldonado, Morillo-Velarde et al. discuss Octopus insularis as a new marine model for evolutionary developmental biology.