RESEARCH ARTICLES
- Whole-transcriptome splicing profiling of E7.5 mouse primary germ layers reveals frequent alternative promoter usage during mouse early embryogenesis
Summary: This study seeks to capture the alternative splicing landscape during mouse gastrulation, underlining the potential importance of alternative promoter usage in mammalian early embryogenesis.
- Separate roles for Med12 and Wnt signaling in regulation of oxytocin expression
Summary: Mediator 12, a transcriptional coactivator, greatly enhances Wnt signaling in the developing embryo. Separate from its role in Wnt signaling, Mediator 12 is required for oxytocin expression.
- Inhibition of miR-34a-5p alleviates hypoxia-reoxygenation injury by enhancing autophagy in steatotic hepatocytes
Summary: Our study establishes an in vitro model for studying H/R injury in fatty liver, and shows that MiR-34a plays an important role in worsening H/R injury, likely by suppressing autophagy in steatotic hepatocytes.
- Degradation of cyclin B is critical for nuclear division in Trypanosoma brucei
Summary: Trypanosomes do not have a spindle checkpoint but have an ability to regulate the timing of nuclear division by modulating the cyclin B protein level.
- The cross-tissue metabolic response of abalone (Haliotis midae) to functional hypoxia
Summary: We report, for the first time, a metabolic map of abalone metabolism in response to functional hypoxia, compiled from results obtained by metabolomics analysis.
- The teneurin C-terminal domain possesses nuclease activity and is apoptogenic
Summary: Teneurins are transmembrane proteins found in the developing nervous system that are related to bacterial toxins. Teneurins also have cytotoxic properties that may help regulate apoptosis or pruning.
- pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas)
Summary: Oyster sperm motility is not triggered in an acidic environment and motility is controlled by internal pH alkalinization, mediated by a Na+/H+ exchanger.
- ZFP36L2 is a cell cycle-regulated CCCH protein necessary for DNA lesion-induced S-phase arrest
Summary: ZFP36L2 is a cell cycle-regulated RNA-binding protein, the abundance of which is regulated post-translationally. This protein is especially accumulated in and critical for the survival of DNA-damaged cells.
- Adjustable delivery of pro-angiogenic FGF-2 by alginate:collagen microspheres
Summary: The development of alginate:collagen composite hydrogel microspheres of adjustable size and degradation speed is described as a new platform for delivery of pro-angiogenic FGF-2 or pro-angiogenic cells.
- A novel reporter allele for monitoring Dll4 expression within the embryonic and adult mouse
Summary: We have developed a novel reporter line, free from complicating factors associated with previous alleles, for monitoring Dll4 expression, at a cellular resolution, in the developing and adult mouse.
METHODS & TECHNIQUES
- Streamlined histone-based fluorescence lifetime imaging microscopy (FLIM) for studying chromatin organisation
Summary: A streamlined and adaptable cell imaging method for quantitative analysis of chromatin organisation in mammalian cells, and its application, reveal a new role for ATM in regulating chromatin structure.
- Establishment and long-term maintenance of primary intestinal epithelial cells cultured from the rainbow trout, Oncorhynchus mykiss
Summary: Understanding chemical uptake from the diet is difficult in live fish: we developed long-term intestinal cell cultures that enables the science and provides an alternative method.
This issue
Other journals from The Company of Biologists
First person interview – Mary Salcedo
Have you seen our interviews with early-career first authors of our papers? In the latest issue, first author Mary Salcedo discusses her latest Methods and Techniques paper in which a newly created method geometrically analyses 789 insect wings.
Find out how Pamela Imperadore’s Travelling Fellowship grant from The Company of Biologists took her to Germany, where she used new imaging techniques to investigate the cellular machinery underlying octopus arm regeneration. Don’t miss the next application deadline for 2020 travel, coming up on 29 November. Where will your research take you?
On the cover, David Coppola, Brent Craven and their colleagues refute a widely-debated theory of olfactory coding first suggested by the Nobel Laureate Lord Adrian over half a century ago.
Biology Open has strong credentials and publishing with us is easy and fast. BiO aims to provide rapid publication for scientifically sound observations and valid conclusions in developmental, cell, experimental and translational biology. Submit your paper here – you’ll be in good company!
Recent cell science highlights in BiO – Masamitsu Sato and colleagues show that the conserved microtubule-associated protein CLASP facilitates bundling of spindle microtubules in early mitosis.
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preLights - Mammalian Y RNAs are modified at discrete guanosine residues with N-glycans
Connor Rosen discusses his selected preprint by Carolyn Bertozzi, Ryan Flynn and their co-workers on the glycosylation of small non-coding RNAs in mammalian cells.