RESEARCH ARTICLES
- Whole-transcriptome splicing profiling of E7.5 mouse primary germ layers reveals frequent alternative promoter usage during mouse early embryogenesis
Summary: This study seeks to capture the alternative splicing landscape during mouse gastrulation, underlining the potential importance of alternative promoter usage in mammalian early embryogenesis.
- Separate roles for Med12 and Wnt signaling in regulation of oxytocin expression
Summary: Mediator 12, a transcriptional coactivator, greatly enhances Wnt signaling in the developing embryo. Separate from its role in Wnt signaling, Mediator 12 is required for oxytocin expression.
- Inhibition of miR-34a-5p alleviates hypoxia-reoxygenation injury by enhancing autophagy in steatotic hepatocytes
Summary: Our study establishes an in vitro model for studying H/R injury in fatty liver, and shows that MiR-34a plays an important role in worsening H/R injury, likely by suppressing autophagy in steatotic hepatocytes.
- Degradation of cyclin B is critical for nuclear division in Trypanosoma brucei
Summary: Trypanosomes do not have a spindle checkpoint but have an ability to regulate the timing of nuclear division by modulating the cyclin B protein level.
- The cross-tissue metabolic response of abalone (Haliotis midae) to functional hypoxia
Summary: We report, for the first time, a metabolic map of abalone metabolism in response to functional hypoxia, compiled from results obtained by metabolomics analysis.
- The teneurin C-terminal domain possesses nuclease activity and is apoptogenic
Summary: Teneurins are transmembrane proteins found in the developing nervous system that are related to bacterial toxins. Teneurins also have cytotoxic properties that may help regulate apoptosis or pruning.
- pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas)
Summary: Oyster sperm motility is not triggered in an acidic environment and motility is controlled by internal pH alkalinization, mediated by a Na+/H+ exchanger.
- ZFP36L2 is a cell cycle-regulated CCCH protein necessary for DNA lesion-induced S-phase arrest
Summary: ZFP36L2 is a cell cycle-regulated RNA-binding protein, the abundance of which is regulated post-translationally. This protein is especially accumulated in and critical for the survival of DNA-damaged cells.
- Adjustable delivery of pro-angiogenic FGF-2 by alginate:collagen microspheres
Summary: The development of alginate:collagen composite hydrogel microspheres of adjustable size and degradation speed is described as a new platform for delivery of pro-angiogenic FGF-2 or pro-angiogenic cells.
- A novel reporter allele for monitoring Dll4 expression within the embryonic and adult mouse
Summary: We have developed a novel reporter line, free from complicating factors associated with previous alleles, for monitoring Dll4 expression, at a cellular resolution, in the developing and adult mouse.
METHODS & TECHNIQUES
- Streamlined histone-based fluorescence lifetime imaging microscopy (FLIM) for studying chromatin organisation
Summary: A streamlined and adaptable cell imaging method for quantitative analysis of chromatin organisation in mammalian cells, and its application, reveal a new role for ATM in regulating chromatin structure.
- Establishment and long-term maintenance of primary intestinal epithelial cells cultured from the rainbow trout, Oncorhynchus mykiss
Summary: Understanding chemical uptake from the diet is difficult in live fish: we developed long-term intestinal cell cultures that enables the science and provides an alternative method.
This issue
Other journals from The Company of Biologists
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Catch up with our first authors in the latest interviews. Meghan Barrett, Katherine Fiocca, Anand Singh, Hagen Gegner and Ling Wang discuss the implications of their research, life as an early-career researcher and their next steps.
Research by Gegner et al. uses targeted metabolomics and physiological measurements to explain the increased bleaching resistance of symbiotic Aiptasia exposed to high salinity.
Why publish with BiO?
Biology Open aims to provide rapid publication for scientifically sound observations and valid conclusions in developmental, cell, experimental and translational biology. Submit your paper for an easy and fast publication process. Recent disease modelling highlights in in BiO include work by Liu et al., which shows that zebrafish is a powerful in vivo model for investigating cardiomyopathy genetics.