cdc14 null males exhibit decreased sperm competition. (B) A control experiment was performed using white-eyed y w males for both the first and second males. A second control experiment was performed using red-eyed cdc14 null males for both the first and second males. The cdc14 null males are less competitive compared to control males regardless of whether they are the first or second male to mate. Results for a single representative replicates (n≥15 vials per cross) are shown. Additional data can be found in Fig. S5B. Data were analyzed using a Chi-squared test with Bonferroni correction. Six pairwise comparisons were made. Red-eyed control males were compared to the cdc14 null, rescue, or overexpression males; cdc14 null males were compared to rescue or overexpression male; and rescue males were compared to overexpression males. *P<0.009. ***P<0.0002.

cdc14 null males mate overnight at the same rate as controls. (B) Eye color of offspring from all replicates of the sperm competition assay. The proportion of offspring from cdc14 null males was significantly lower than the control (red). This decrease was rescued by expression of nos>myc-cdc14. Data were analyzed by Chi-squared test with Bonferroni correction. Control (red) was compared to the cdc14 null, rescue, and overexpression. The cdc14 null was compared to the rescue. *ρ<0.02, **ρ<0.003, ***ρ<0.0003. In (A) and (B), N≥48 vials aggregated in N≥3 experiments.