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Research Article
rebuff regulates apical luminal matrix to control tube size in Drosophila trachea
Rachana R. Chandran, Aaron Scholl, Yuyang Yang, Lan Jiang
Biology Open 2018 7: bio036848 doi: 10.1242/bio.036848 Published 5 September 2018
Rachana R. Chandran
Department of Biological Sciences, Oakland University, 2200 N. Squirrel Road, Rochester, MI 48309, USA
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Aaron Scholl
Department of Biological Sciences, Oakland University, 2200 N. Squirrel Road, Rochester, MI 48309, USA
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  • ORCID record for Aaron Scholl
Yuyang Yang
Department of Biological Sciences, Oakland University, 2200 N. Squirrel Road, Rochester, MI 48309, USA
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Lan Jiang
Department of Biological Sciences, Oakland University, 2200 N. Squirrel Road, Rochester, MI 48309, USA
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  • For correspondence: Jiang23@oakland.edu
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ABSTRACT

The Drosophila embryonic tracheal network is an excellent model to study tube size. The chitin-based apical luminal matrix and cell polarity are well known to regulate tube size in Drosophila trachea. Defects in luminal matrix and cell polarity lead to tube overexpansion. Here, we address the novel function of the rebuff (reb) gene, which encodes an evolutionarily conserved Smad-like protein. In reb mutants, tracheal tubes are moderately over-elongated. Despite the establishment of normal cell polarity, we observed significantly reduced apical luminal matrix in reb mutants. Among various luminal components, luminal Obstructor-A (ObstA) is drastically reduced. Interestingly, ObstA is localized in vesicle-like structures that are apically concentrated in reb mutants. To investigate the possibility that reb is involved in the endocytosis of ObstA, we analyzed the co-localization of ObstA and endocytic markers in reb mutants. We observed that ObstA is localized in late endosomes and recycling endosomes. This suggests that in reb mutant trachea, endocytosed ObstA is degraded or recycled back to the apical region. However, ObstA vesicles are retained in the apical region and are failed to be secreted to the lumen. Taken together, these results suggest one function of reb is regulating the endocytosis of luminal matrix components.

Footnotes

  • Competing interests

    The authors declare no competing or financial interests.

  • Author contributions

    Conceptualization: L.J.; Methodology: R.R.C., L.J.; Formal analysis: R.R.C., L.J.; Investigation: R.R.C., A.S., Y.Y., L.J.; Writing - original draft: L.J.; Writing - review & editing: A.S., L.J.; Supervision: L.J.; Project administration: L.J.; Funding acquisition: L.J.

  • Funding

    This project was supported by the Oakland University Research Excellence Fund 10126 and National Institute of Health R15GM100369 grant to L.J.

  • Received June 25, 2018.
  • Accepted July 19, 2018.
  • © 2018. Published by The Company of Biologists Ltd
http://creativecommons.org/licenses/by/3.0

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Keywords

  • rebuff
  • Luminal matrix
  • Endocytosis
  • ObstA

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Research Article
rebuff regulates apical luminal matrix to control tube size in Drosophila trachea
Rachana R. Chandran, Aaron Scholl, Yuyang Yang, Lan Jiang
Biology Open 2018 7: bio036848 doi: 10.1242/bio.036848 Published 5 September 2018
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Research Article
rebuff regulates apical luminal matrix to control tube size in Drosophila trachea
Rachana R. Chandran, Aaron Scholl, Yuyang Yang, Lan Jiang
Biology Open 2018 7: bio036848 doi: 10.1242/bio.036848 Published 5 September 2018

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