RESEARCH ARTICLES
- Unanchored ubiquitin chains do not lead to marked alterations in gene expression in Drosophila melanogaster
Summary: Our Drosophila studies indicate the lack of a marked, coordinated response towards unanchored poly-ubiquitin in flies, suggesting that untethered ubiquitin chains are not necessarily problematic in intact organisms.
- Re-evaluation of the causes of variation among mouse aggregation chimaeras
Summary: Analysis of published results suggests that two stochastic allocation steps in chimaeric mouse blastocysts, without the geometrical sampling mechanism proposed previously, could explain much of the variation among aggregation chimaeras.
- Numb regulates cell tension required for mammary duct elongation
Summary: Numb regulates cortical cell tension, which is necessary to maintain proper cell shape and packing density during mammary duct elongation.
- The optimal mechanical condition in stem cell-to-tenocyte differentiation determined with the homogeneous strain distributions and the cellular orientation control
Summary: We determined the relationship between the cyclic strain of culture membrane and cellular gene expression, from which the most effective strain for tenogenic differentiation of stem cells was elucidated.
- Cold adaptive potential of pine wood nematodes overwintering in plant hosts
Summary: The modifications in fatty acid composition and cryoprotectant levels, as elements of its changing physiology, play important roles in the overwintering success of the pine wood nematode.
- Physiological and behavioral stress responses to predators are altered by prior predator experience in juvenile qingbo (Spinibarbus sinensis)
Summary: A predator-naive carp can recognize its natural predator, and this recognition can be intensified by prior experience with a predator or the presence of a conspecific.
- Defining the dynamic chromatin landscape of mouse nephron progenitors
Summary: An investigation of the chromatin landscape of mouse nephron progenitors across their life span supports the view that old nephron progenitors are epigenetically poised for differentiation.
- Tpl2 is required for VEGF-A-stimulated signal transduction and endothelial cell function
Summary: Our study shows that the growth factor VEGF-A stimulates synthesis in endothelial cells of a proto-oncoprotein and protein kinase, Tpl2, and this is required for signal transduction and angiogenesis.
- Blockage of glutamine-dependent anaplerosis affects mTORC1/2 activity and ultimately leads to cellular senescence-like response
Summary: Glutamine-dependent anaplerosis is essential to cell growth and closely associated with mTORC1 activation and mTORC2 inactivation, and impedes cellular senescence particularly associated with p16INK4A.
- Dietary iron restriction leads to a reduction in hepatic fibrosis in a rat model of non-alcoholic steatohepatitis
Summary: We reveal that dietary iron restriction leads to a reduction in hepatic inflammation, oxidative stress and fibrosis in rats fed a choline-deficient L-amino acid-defined (CDAA) diet.
- Quantitative proteomic profiling of tumor-associated vascular endothelial cells in colorectal cancer
Summary: We provided large-scale proteomic profiling of vascular endothelial cells in colorectal cancer with quantitative information, a number of potential antiangiogenic targets and a novel vision in the angiogenesis bio-mechanism of CRC.
- Xenotransplantation of Human glioblastoma in Zebrafish larvae: in vivo imaging and proliferation assessment
Summary: The combination of light sheet fluorescent microscopy and flow cytometry allows assessment of proliferation and tumor growth of human GBM inside zebrafish, making it a useful model to identify effective anti-proliferative agents in a preclinical setting.
- Effects of chronic inhibition of Testosterone metabolism on cardiac remodeling after ischemia/reperfusion-induced myocardial damage in gonadectomized rats
Summary: Coronary ischemia/reperfusion-induced injury in gonadectomyzed male rats is decreased by testosterone, protection is increased by blocking its 5α-reduction and blocked by inhibition of its aromatization.
- Pioglitazone abolishes cognition impairments as well as BDNF and neurotensin disturbances in a rat model of autism
Summary: Exposure of rats to lipopolysaccharide during gestation induced autistic-like behaviors in the juvenile offspring. Daily postnatal pioglitazone treatment abolished cognition impairments as well as brain-derived neurotrophic factor and neurotensin disturbances.
- Meiosis in the scorpion Tityus silvestris: new insights into achiasmatic chromosomes
Summary: The scorpion Tityus silvestris, despite not presenting chiasma, performs initial stages of the recombination process and maintains the homologues synapse and different epigenetic modifications until late stages of meiosis I.
- Karyoevolution of Crenicichla heckel 1840 (Cichlidae, Perciformes): a process mediated by inversions
Summary: This paper has a great importance for understanding karyotype evolutionary dynamics in neotropical freshwater fish, focusing on repetitive DNA and the role of inversions in Crenicichla.
- Longitudinal characterization of diet-induced genetic murine models of non-alcoholic steatohepatitis with metabolic, histological, and transcriptomic hallmarks of human patients
Summary: We revealed that ob/ob mice under fast food diet (FFD) regime develop metabolic, histological and transcriptomic dysfunctions similar to human non-alcoholic steatohepatitis (NASH).
- Hedgehog signaling regulates osteoblast differentiation in zebrafish larvae through modulation of autophagy
Summary: Our report of an essential regulation role of hedgehog signaling and autophagy on osteoblast differentiation may contribute to research on bone development biology, hedgehog signaling and the autophagy pathway.
- Exosomal transfer of bone marrow mesenchymal stem cell-derived miR-340 attenuates endometrial fibrosis
Summary: miR-340 in the exosomes released from BMSCs are transferred to endometrial cells, which regulate gene expression, repress endometrial fibrosis and promote functional recovery in rats subjected to mechanical damage.
- Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts
Summary: Fundamental physiology of the bovine trophectoderm and hallmarks of the self-renewing undifferentiated ‘trophoblast stem cells’ are interpreted through systematic analysis of the cellular proteome and transcriptome.
- Intercellular transfer of P-glycoprotein mediates the formation of stable multi-drug resistance in human bladder cancer BIU-87 cells
Summary: Intercellular transfer of P-glycoprotein forms stable multi-drug resistance, which complements the formation mechanism of MDR and indicates that new treatment strategies need to be designed to overcome MDR.
METHODS AND TECHNIQUES
- SuperCLEM: an accessible correlative light and electron microscopy approach for investigation of neurons and glia in vitro
Summary: We developed an advanced, accessible and adaptable correlative light and electron microscopy pipeline for study of neuron-glia cultures and demonstrate its power by deriving accurate 3D models of myelinated axons.
- Beyond the whole-mount phenotype: high-resolution imaging in fluorescence-based applications on zebrafish
Summary: We propose a GMA embedding method that preserves fluorescent signals without the need for antibodies. The method complements whole-mount methods especially if advanced imaging technology is not readily available.
- Detection of changes in mitochondrial hydrogen sulfide in vivo in the fish model Poecilia mexicana (Poeciliidae)
Summary: We established the mitochondria-targeted mass spectrometry probe MitoA for H2S detection in vivo in the animal model Poecilia mexicana for the first time.
- High-yield isolation of menstrual blood-derived endometrial stem cells by direct red blood cell lysis treatment
Summary: MenSC isolation by directly lysing the red blood cells not only guarantees substantial amounts of superior MenSCs with low passage number, but also is time efficient and economical.