RESEARCH ARTICLES
- Multi-modal and multiscale imaging approaches reveal novel cardiovascular pathophysiology in Drosophila melanogaster
Summary: Mutant troponin I increases aortic and myocardial stiffness and engenders histologic abnormalities in fruit flies.
- Accuracy of fruit-fly eclosion rhythms evolves by strengthening circadian gating rather than developmental fine-tuning
Summary: Fruit fly populations that have evolved greater accuracy of eclosion rhythms show enhanced gating of their eclosion by circadian clocks but not reduced variation in clock-independent developmental processes.
- Unique trophoblast chromatin environment mediated by the PcG protein SFMBT2
Summary: The PcG protein SFMBT2, essential for placenta development, is distributed mainly to repetitive elements in trophoblast stem cells, arguing for an architectural role in stem cell maintenance.
- A Plasmodium berghei putative serine-threonine kinase 2 (PBANKA_0311400) is required for late liver stage development and timely initiation of blood stage infection
Summary: Requirement of a putative serine threonine kinase 2 (PBANKA_0311400) for Plasmodium berghei liver stage development.
- EGFP insertional mutagenesis reveals multiple FXR2P fibrillar states with differing ribosome association in neurons
Summary: A mutagenesis study reveals that the higher-order structural states of the RBP FXR2P in neurons can be regulated by manipulation of the LC and RNA-binding domains.
- Role of Dach1 revealed using a novel inner ear-specific Dach1-knockdown mouse model
Summary: Inner ear-specific downregulation of Dach1 reveals that the epithelial–mesenchymal transition is crucial for the generation of the stria vascularis.
- Mechanical stress-induced cell death in breast cancer cells
Summary: Dynamic mechanical compressive stress loading applied to two different breast cancer cell lines results in mechanical stress-induced cell damage (MSICD) composed of apoptosis and necrosis.
- Exposure to a 50 Hz magnetic field at 100 µT exerts no DNA damage in cardiomyocytes
Summary: The current study shows that neither continuous nor intermittent exposure to a 50 Hz magnetic field at 100 µT induces DNA damage in cardiomyocytes.
- Inhalation of marijuana affects Drosophila heart function
Summary: Marijuana induces early arrhythmogenesis and increases contractility in Drosophila. Our approach using this animal model allows us to carry out screenings to explore the impact of cannabinoids.
- The ventral epithelium of Trichoplax adhaerens deploys in distinct patterns cells that secrete digestive enzymes, mucus or diverse neuropeptides
Summary: The external digestive epithelium of the primitive animal Trichoplax adhaerens includes secretory cell types and secretory products much like those in the internal digestive systems of other animals.
- Independent evolution for sex determination and differentiation in the DMRT family in animals
Summary: Three DMRT family genes, vertebrate dmrt1, arthropod dsx and nematode mab-3, involved in sex determination and primary sex differentiation have no orthologous relationships, indicating independent evolution in bilaterian animals.
- HDAC3 and HDAC8 are required for cilia assembly and elongation
Summary: We identified that HDAC3 and HDAC8 are required for the assembly and elongation of the primary cilia.
- YFR016c/Aip5 is part of an actin nucleation complex in yeast
Summary: YFR016c/Aip5 binds to the polarisome components Bud6 and Spa2 and supports the polarisome in the formation of actin cables in yeast cells.
- Discoidin domain receptor 1 deficiency in vascular smooth muscle cells leads to mislocalisation of N-cadherin contacts
Summary: Here we show for the first time that discoidin domain receptor 1 associates with and stabilizes N-cadherin cell–cell contacts in vascular smooth muscle cells.
- The Col4a2em1(IMPC)Wtsi mouse line: lessons from the Deciphering the Mechanisms of Developmental Disorders program
Summary: We define the spectrum of phenotypic abnormalities linked with Col4a2 disruption and demonstrate the opportunities the Deciphering the Mechanisms of Developmental Disorders (DMDD) program offers for exploring rare human diseases.
- MicroRNA-138 negatively regulates the hypoxia-inducible factor 1α to suppress melanoma growth and metastasis
Summary: Our study demonstrates the role and clinical relevance of miRNA-138 and HIF1α in melanoma cell growth and metastasis, providing a novel therapeutic target for suppression of melanoma growth and metastasis.
- Reduced mTORC1-signalling in retinal progenitor cells leads to visual pathway dysfunction
Summary: Conditional deletion of Rptor in retinal progenitor cells demonstrates that mTORC1-signalling is critical for visual pathway development and function.
- Comparing 3D ultrastructure of presynaptic and postsynaptic mitochondria
Summary: Our 3D ultrastructural analysis of multiple brain regions shows distinctively different sizes and shapes of mitochondria in the presynaptic terminal and postsynaptic dendrite.
METHODS AND TECHNIQUES
- Three-dimensional analysis of single molecule FISH in human colon organoids
Summary: We present a sensitive single-molecule (sm)FISH application for the identification of individual mRNA transcripts and their spatial distribution in 3D in whole human colon organoids.