RESEARCH ARTICLES
- Cryo-EM of human Arp2/3 complexes provides structural insights into actin nucleation modulation by ARPC5 isoforms
Summary: The Arp2/3 complex stimulates formation of branched actin filament networks and exhibits isoform diversity in mammals. We show how different Arp2/3 subunit isoforms contribute to differences in complex function.
- Cell-extrinsic requirement for sulfate in regulating hippocampal neurogenesis
Summary Statement: Heterozygosity for the brain sulfate transporter Slc13a4 during postnatal development results in life-long abnormal hippocampal neurogenesis.
- HSP70 drives myoblast fusion during C2C12 myogenic differentiation
Summary: In C2C12 myoblasts, HSP70 overexpression does not alter proliferation or differentiation but enhances fusion to increase myotube size.
- The effects of temperature on the proxies of visual detection of Danio rerio larvae: observations from the optic tectum
Summary: During the assessment of the visual detection distance of transgenic Danio rerio larvae in response to a visual stimulus using selective plane illumination microscopy, we found that the distance is shorter at elevated temperatures.
- CTP synthase polymerization in germline cells of the developing Drosophila egg supports egg production
Summary: A point mutation in the nucleotide biosynthetic enzyme CTP synthase dominantly inhibits its polymerization and can be used to suppress CTPS assembly in a tissue-specific manner in vivo. Disrupting assembly in ovarian germline cells reduces egg production, demonstrating a functional role for CTPS assembly.
- Mechanical and energetic determinants of impaired gait following stroke: segmental work and pendular energy transduction during treadmill walking
Summary: Walking after a stroke may be energetically consuming. Here, we show how compensations and asymmetries may contribute to increasing the amount of work needed to walk following a stroke.
- Communication between Cyclin-dependent kinase Cdc2 and the Wis1-Spc1 MAPK pathway determines mitotic timing in Schizosaccharomyces pombe
Summary: Modulation of Cdc2 activity can change the extent of Spc1 activation and preserve mitotic fidelity in Schizosaccharomyces pombe cells.
- Phospholipids containing ether-bound hydrocarbon-chains are essential for efficient phagocytosis and neutral lipids of the ester-type perturb development in Dictyostelium
Summary: Lean Dictyostelium mutants that fail to produce storage fat, survive starvation and succeed in spore formation, whereas cell strains impaired in ether-type membrane lipid production are perturbed in phagocytosis.
- Filament formation by the translation factor eIF2B regulates protein synthesis in starved cells
Summary: Filament formation by eIF2B is an adaptive mechanism that allows for efficient shutdown of translation in starved yeast cells.
- Responses to transient receptor potential (TRP) channel agonists in Chlamydomonas reinhardtii
Summary: Chlamydomonas reinhardtii cells lose motility and excise flagella upon application of pungent TRP channel agonists such as capsaicin and gingerol, likely through TRP channel activity, including ADF1.
- Downregulated brain and muscle aryl hydrocarbon receptor nuclear translocator-like protein-1 inhibits osteogenesis of BMSCs through p53 in type 2 diabetes mellitus
Summary: This article reported for the first time that inhibition of BMSCs osteogenic differentiation by downregulated BMAL1 in T2DM was mediated by p53.
- Brain-encysting trematodes (Euhaplorchis californiensis) decrease raphe serotonergic activity in California killifish (Fundulus parvipinnis)
Summary: Post-stress 5-HT signalling decrease with parasite density in killifish infected with brain-dwelling Euhaplorchis californiensis metacercariae, in a manner consistent with decreased impulse control and increased risk taking in infected fish.
METHODS & TECHNIQUES
- Going beyond conventional parameters to unveil sperm quality in fish: the use of fibre optic technology to assess mitochondrial respiratory performance
Summary: A novel technique to measure sperm mitochondrial respiratory efficiency, an overlooked parameter of sperm quality that might contribute in unveiling mechanisms driving the intraspecific variability in sperm performance.