PT  - JOURNAL ARTICLE
AU  - Han, Xiaolei
AU  - Wang, Tiedong
AU  - Zhang, Jifeng
AU  - Liu, Xingxing
AU  - Li, Zhuang
AU  - Wang, Gangqi
AU  - Song, Qi
AU  - Pang, Daxin
AU  - Ouyang, Hongsheng
AU  - Tang, Xiaochun
TI  - Apolipoprotein CIII regulates lipoprotein-associated phospholipase A<sub>2</sub> expression via the MAPK and NFκB pathways
AID  - 10.1242/bio.201410900
DP  - 2015 May 15
TA  - Biology Open
PG  - 661--665
VI  - 4
IP  - 5
4099  - http://bio.biologists.org/content/4/5/661.short
4100  - http://bio.biologists.org/content/4/5/661.full
SO  - Biology Open2015 May 15; 4
AB  - Apolipoprotein CIII (apo CIII), a small glycoprotein that binds to the surfaces of certain lipoproteins, is associated with inflammatory and atherogenic responses in vascular cells. Lipoprotein-associated phospholipase A2 (Lp-PLA2) has been proposed as an inflammatory biomarker and potential therapeutic target for cardiovascular disease (CVD). Here, we report that apo CIII increases Lp-PLA2 mRNA and protein levels in dose- and time- dependent manner in human monocytic THP-1 cells, and the increase can be abolished by MAPK and NFκB pathway inhibitors. Lp-PLA2 inhibitor, 1-linoleoyl glycerol attenuates the inflammation induced by apo CIII. In turn, exogenous Lp-PLA2 expression upregulates apo CIII and the upregulation can be inhibited by 1-linoleoyl glycerol in HepG2 cells. Moreover, plasma Lp-PLA2 level is correlated with apo CIII expression in pig liver. In vivo, Lp-PLA2 expression in monocytes and its activity in serum were significantly increased in human apo CIII transgenic porcine models compared with wild-type pigs. Our results suggest that Lp-PLA2 and apo CIII expression level is correlated with each other in vitro and in vivo.