RT Journal Article
SR Electronic
T1 Regulatory RNPs: a novel class of ribonucleoproteins that potentially contribute to ribosome heterogeneity
JF Biology Open
JO Biology Open
FD Company of Biologists
SP 1342
OP 1354
DO 10.1242/bio.028092
VO 6
IS 9
A1 Poole, Aaron R.
A1 Vicino, Ian
A1 Adachi, Hironori
A1 Yu, Yi-Tao
A1 Hebert, Michael D.
YR 2017
UL http://bio.biologists.org/content/6/9/1342.abstract
AB Many ribonucleoproteins (RNPs), which are comprised of noncoding RNA and associated proteins, are involved in essential cellular processes such as translation and pre-mRNA splicing. One class of RNP is the small Cajal body-specific RNP (scaRNP), which contributes to the biogenesis of small nuclear RNPs (snRNPs) that are central components of the spliceosome. Three scaRNAs are internally processed, generating stable nucleolus-enriched RNAs of unknown function. Here, we provide data that show that these RNAs become part of RNPs we term regulatory RNPs (regRNPs). Most modifications within rRNA (predominantly pseudouridylation and ribose 2′-O-methylation) are conducted by small nucleolar RNPs (snoRNPs), and we provide evidence that the activity of at least some of these snoRNPs is under the control of regRNPs. Because modifications within rRNA can vary in different physiological or pathological situations, rRNA modifications are thought to be the major source of ribosome heterogeneity. Our identification of regRNPs thus provides a potential mechanism for how ribosome heterogeneity may be accomplished. This work also provides additional functional connections between the Cajal body and the nucleolus.