PT  - JOURNAL ARTICLE
AU  - Pinsino, Annalisa
AU  - Alijagic, Andi
TI  - Sea urchin <em>Paracentrotus lividus</em> immune cells in culture: formulation of the appropriate harvesting and culture media and maintenance conditions
AID  - 10.1242/bio.039289
DP  - 2019 Mar 15
TA  - Biology Open
PG  - bio039289
VI  - 8
IP  - 3
4099  - http://bio.biologists.org/content/8/3/bio039289.short
4100  - http://bio.biologists.org/content/8/3/bio039289.full
SO  - Biology Open2019 Mar 15; 8
AB  - The sea urchin is an emergent model system for studying basic and translational immunology. Here we report a new method for the harvesting and maintenance of primary immune cells isolated from adult Paracentrotus lividus, a common Mediterranean sea urchin species. This optimised method uses coelomocyte culture medium, containing a high-affinity Ca2+ chelator, as the ideal harvesting and anti-clotting vehicle and short-term culture medium (≤48 h), and artificial seawater as the master medium that maintains cell survival and in vitro-ex vivo physiological homeostasis over 2 weeks. Gradually reducing the amount of anticoagulant solution in the medium and regularly replacing the medium led to improved culture viability. Access to a robust and straightforward in vitro-ex vivo system will expedite our understanding of deuterostome immunity as well as underscore the potential of sea urchin with respect to biomedicine and regulatory testing.This article has an associated First Person interview with the first author of the paper.