RT Journal Article
SR Electronic
T1 Monitoring <em>Schizosaccharomyces pombe</em> genome stress by visualizing end-binding protein Ku
JF Biology Open
JO Biology Open
FD Company of Biologists
SP bio054346
DO 10.1242/bio.054346
VO 10
IS 2
A1 Jones, Chance E.
A1 Forsburg, Susan L.
YR 2021
UL http://bio.biologists.org/content/10/2/bio054346.abstract
AB Studies of genome stability have exploited visualization of fluorescently tagged proteins in live cells to characterize DNA damage, checkpoint, and repair responses. In this report, we describe a new tool for fission yeast, a tagged version of the end-binding protein Pku70 which is part of the KU protein complex. We compare Pku70 localization to other markers upon treatment to various genotoxins, and identify a unique pattern of distribution. Pku70 provides a new tool to define and characterize DNA lesions and the repair response.