Autophagy
- Assessing methods to quantitatively validate TGFβ-dependent autophagy
Summary: Using various tools including western blotting and immunofluorescence microscopy, it was observed that autophagic flux probes are the most robust tools to quantitatively assess cellular autophagy.
- Targeted inhibition of GRP78 by HA15 promotes apoptosis of lung cancer cells accompanied by ER stress and autophagy
Summary: GRP78 is highly expressed in the lung cancer tissue, and targeted inhibition of GRP78 by HA15 promotes apoptosis of lung cancer cells accompanied by endoplasmic reticulum stress and autophagy.
- Analysis of a cellular structure observed in the compound eyes of Drosophila white; yata mutants and white mutants
Summary: We analysed a cellular structure formed by changes associated with exposure to light in the compound eyes of Drosophila white mutants, white; yata mutants and aged flies.
- Autophagy-related Djatg8 is required for remodeling in planarian Dugesia japonica
Summary: The autophagy-related Djatg8 homologues Djatg8-2 and Djatg8-3 are required for planarian remodeling; we provide an emergent in vivo model organism to study autophagy and the molecular mechanism for planarian remodeling.
- Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via the AMPK-mTOR pathway
Summary: We prove that exogenous H2S can suppress NLRP3-mediated inflammation by promoting autophagy via the AMPK/mTOR pathway in L02 cells, providing insights into the anti-inflammatory mechanism of H2S.
- Hedgehog signaling regulates osteoblast differentiation in zebrafish larvae through modulation of autophagy
Summary: Our report of an essential regulation role of hedgehog signaling and autophagy on osteoblast differentiation may contribute to research on bone development biology, hedgehog signaling and the autophagy pathway.
- The chromodomain helicase CHD4 regulates ERBB2 signaling pathway and autophagy in ERBB2+ breast cancer cells
Summary: Inhibition of CHD4 expression impairs cell proliferation and survival through downregulation of ERBB2 signaling and block of autophagy. Therefore, CHD4 should be considered a potential target in ERBB2+ breast cancers.
- Survivin inhibits excessive autophagy in cancer cells but does so independently of its interaction with LC3
Summary: Survivin binds to LC3 through a canonical LIR, yet this interaction is not required for survivin overexpression to reduce autophagic flux, suggesting that survivin regulates autophagy via an alternative mechanism.
- Inhibition of miR-34a-5p alleviates hypoxia-reoxygenation injury by enhancing autophagy in steatotic hepatocytes
Summary: Our study establishes an in vitro model for studying H/R injury in fatty liver, and shows that MiR-34a plays an important role in worsening H/R injury, likely by suppressing autophagy in steatotic hepatocytes.
- Distribution of H3K27me3, H3K9me3, and H3K4me3 along autophagy-related genes highly expressed in starved zebrafish myotubes
Summary: The role of nuclear events in the control of autophagy is underappreciated. This study demonstrates cell starvation associated gene expression changes and autophagy-specific histone modifications.