CRISPR/Cas9
- Recent developments of tools for genome and metabolome studies in basidiomycete fungi and their application to natural product research
Summary: Fungi of the phylum Basidiomycota can make bioactive natural products. We discuss recent advances of tools for genome and metabolome studies that will facilitate natural product discovery and characterisation.
- Efficient and crucial quality control of HAP1 cell ploidy status
Summary: Sharing an effective procedure to quality control the near-haploid HAP1 cells for standardized comparison to CRISPR/Cas9 modified versions and demonstrating the need for controlling the spontaneous diploidization of HAP1 cultures.
- Restriction of mitochondrial calcium overload by mcu inactivation renders a neuroprotective effect in zebrafish models of Parkinson's disease
Summary: Mitochondrial calcium overload causes mitochondrial dysfunction in Parkinson's disease. Inactivation of mitochondrial calcium uniporter reverses mitochondrial calcium overload and rescues dopaminergic neurons in our zebrafish models of Parkinson's disease.
- Divergent Hemogen genes of teleosts and mammals share conserved roles in erythropoiesis: analysis using transgenic and mutant zebrafish
Summary: Transgenic and mutant zebrafish lines were created to characterize the expression and functions of Hemogen, a transcription factor involved in the formation of red blood cells and other processes.
- Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells
Summary: In this paper, we report an efficient genome editing method for cultured medaka fish cells using pre-formed CRISPR/Cas9 RNP. This method will be very useful for gene function studies using cultured fish cells.
- Dlx3b/4b is required for early-born but not later-forming sensory hair cells during zebrafish inner ear development
Summary: The transcription factors Dlx3b and Dlx4b control the formation of early-born sensory hair cells or tether cells in the developing zebrafish inner ear.
- Genetic deletion of amphiregulin restores the normal skin phenotype in a mouse model of the human skin disease tylosis
Summary: Gain-of-function mutations in RHBDF2 cause tylosis, a skin disease characterized by hyperproliferation of keratinocytes. We generated mice carrying the human tylosis disease mutation p.P189L and show that enhanced amphiregulin secretion underlies tylosis.
- Utilising polymorphisms to achieve allele-specific genome editing in zebrafish
Summary: Heterozygous single nucleotide polymorphisms in CRISPR/Cas9 target sites bias genome editing in favour of alleles with perfect complementarity to gRNAs, a feature which can be exploited for chromosome-specific editing.