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CRISPR/Cas9

  • FUTURE LEADER REVIEW
    Recent developments of tools for genome and metabolome studies in basidiomycete fungi and their application to natural product research
    Fabrizio Alberti, Saraa Kaleem, Jack A. Weaver
    Biology Open 2020 9: bio056010 doi: 10.1242/bio.056010 Published 2 December 2020

    Summary: Fungi of the phylum Basidiomycota can make bioactive natural products. We discuss recent advances of tools for genome and metabolome studies that will facilitate natural product discovery and characterisation.

  • METHODS & TECHNIQUES
    Efficient and crucial quality control of HAP1 cell ploidy status
    Tobias B. Beigl, Ine Kjosås, Emilie Seljeseth, Nina Glomnes, Henriette Aksnes
    Biology Open 2020 9: bio057174 doi: 10.1242/bio.057174 Published 12 November 2020

    Summary: Sharing an effective procedure to quality control the near-haploid HAP1 cells for standardized comparison to CRISPR/Cas9 modified versions and demonstrating the need for controlling the spontaneous diploidization of HAP1 cultures.

  • RESEARCH ARTICLE
    Restriction of mitochondrial calcium overload by mcu inactivation renders a neuroprotective effect in zebrafish models of Parkinson's disease
    Smijin K. Soman, Michal Bazała, Marcus Keatinge, Oliver Bandmann, Jacek Kuznicki
    Biology Open 2019 8: bio044347 doi: 10.1242/bio.044347 Published 15 October 2019

    Summary: Mitochondrial calcium overload causes mitochondrial dysfunction in Parkinson's disease. Inactivation of mitochondrial calcium uniporter reverses mitochondrial calcium overload and rescues dopaminergic neurons in our zebrafish models of Parkinson's disease.

  • RESEARCH ARTICLE
    Divergent Hemogen genes of teleosts and mammals share conserved roles in erythropoiesis: analysis using transgenic and mutant zebrafish
    Michael J. Peters, Sandra K. Parker, Jeffrey Grim, Corey A. H. Allard, Jonah Levin, H. William Detrich III
    Biology Open 2018 7: bio035576 doi: 10.1242/bio.035576 Published 28 August 2018

    Summary: Transgenic and mutant zebrafish lines were created to characterize the expression and functions of Hemogen, a transcription factor involved in the formation of red blood cells and other processes.

  • METHODS & TECHNIQUES
    Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells
    Qizhi Liu, Yongming Yuan, Feng Zhu, Yunhan Hong, Ruowen Ge
    Biology Open 2018 7: bio035170 doi: 10.1242/bio.035170 Published 10 August 2018

    Summary: In this paper, we report an efficient genome editing method for cultured medaka fish cells using pre-formed CRISPR/Cas9 RNP. This method will be very useful for gene function studies using cultured fish cells.

  • RESEARCH ARTICLE
    Dlx3b/4b is required for early-born but not later-forming sensory hair cells during zebrafish inner ear development
    Simone Schwarzer, Sandra Spieß, Michael Brand, Stefan Hans
    Biology Open 2017 6: 1270-1278; doi: 10.1242/bio.026211

    Summary: The transcription factors Dlx3b and Dlx4b control the formation of early-born sensory hair cells or tether cells in the developing zebrafish inner ear.

  • RESEARCH ARTICLE
    Genetic deletion of amphiregulin restores the normal skin phenotype in a mouse model of the human skin disease tylosis
    Vishnu Hosur, Benjamin E. Low, Leonard D. Shultz, Michael V. Wiles
    Biology Open 2017 6: 1174-1179; doi: 10.1242/bio.026260

    Summary: Gain-of-function mutations in RHBDF2 cause tylosis, a skin disease characterized by hyperproliferation of keratinocytes. We generated mice carrying the human tylosis disease mutation p.P189L and show that enhanced amphiregulin secretion underlies tylosis.

  • METHODS & TECHNIQUES
    Utilising polymorphisms to achieve allele-specific genome editing in zebrafish
    Samuel J. Capon, Gregory J. Baillie, Neil I. Bower, Jason A. da Silva, Scott Paterson, Benjamin M. Hogan, Cas Simons, Kelly A. Smith
    Biology Open 2017 6: 125-131; doi: 10.1242/bio.020974

    Summary: Heterozygous single nucleotide polymorphisms in CRISPR/Cas9 target sites bias genome editing in favour of alleles with perfect complementarity to gRNAs, a feature which can be exploited for chromosome-specific editing.

  • RESEARCH ARTICLE
    Various applications of TALEN- and CRISPR/Cas9-mediated homologous recombination to modify the Drosophila genome
    Zhongsheng Yu, Hanqing Chen, Jiyong Liu, Hongtao Zhang, Yan Yan, Nannan Zhu, Yawen Guo, Bo Yang, Yan Chang, Fei Dai, Xuehong Liang, Yixu Chen, Yan Shen, Wu-Min Deng, Jianming Chen, Bo Zhang, Changqing Li, Renjie Jiao
    Biology Open 2014 3: 271-280; doi: 10.1242/bio.20147682

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