Genome editing
- Integral gene drives for population replacement
Summary: Here, we describe and model a novel gene drive strategy – integral gene drive – for population replacement based on the hijacking of endogenous genes.
- Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells
Summary: In this paper, we report an efficient genome editing method for cultured medaka fish cells using pre-formed CRISPR/Cas9 RNP. This method will be very useful for gene function studies using cultured fish cells.
- Utilising polymorphisms to achieve allele-specific genome editing in zebrafish
Summary: Heterozygous single nucleotide polymorphisms in CRISPR/Cas9 target sites bias genome editing in favour of alleles with perfect complementarity to gRNAs, a feature which can be exploited for chromosome-specific editing.