Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Interviews
    • Sign up for alerts
  • About us
    • About BiO
    • Editors and Board
    • Editor biographies
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contact
    • Contact BiO
    • Advertising
    • Feedback
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in

Search

  • Advanced search
Biology Open
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Biology Open

Advanced search

RSS   Twitter   Facebook   YouTube

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Interviews
    • Sign up for alerts
  • About us
    • About BiO
    • Editors and Board
    • Editor biographies
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contact
    • Contact BiO
    • Advertising
    • Feedback

Genome editing

  • RESEARCH ARTICLE
    Integral gene drives for population replacement
    Alexander Nash, Giulia Mignini Urdaneta, Andrea K. Beaghton, Astrid Hoermann, Philippos Aris Papathanos, George K. Christophides, Nikolai Windbichler
    Biology Open 2019 8: bio037762 doi: 10.1242/bio.037762 Published 3 January 2019

    Summary: Here, we describe and model a novel gene drive strategy – integral gene drive – for population replacement based on the hijacking of endogenous genes.

  • METHODS & TECHNIQUES
    Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells
    Qizhi Liu, Yongming Yuan, Feng Zhu, Yunhan Hong, Ruowen Ge
    Biology Open 2018 7: bio035170 doi: 10.1242/bio.035170 Published 10 August 2018

    Summary: In this paper, we report an efficient genome editing method for cultured medaka fish cells using pre-formed CRISPR/Cas9 RNP. This method will be very useful for gene function studies using cultured fish cells.

  • METHODS & TECHNIQUES
    Utilising polymorphisms to achieve allele-specific genome editing in zebrafish
    Samuel J. Capon, Gregory J. Baillie, Neil I. Bower, Jason A. da Silva, Scott Paterson, Benjamin M. Hogan, Cas Simons, Kelly A. Smith
    Biology Open 2017 6: 125-131; doi: 10.1242/bio.020974

    Summary: Heterozygous single nucleotide polymorphisms in CRISPR/Cas9 target sites bias genome editing in favour of alleles with perfect complementarity to gRNAs, a feature which can be exploited for chromosome-specific editing.

  • RESEARCH ARTICLE
    Highly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevis
    Keisuke Nakajima, Yoshio Yaoita
    Biology Open 2015 4: 180-185; doi: 10.1242/bio.201410009
  • RESEARCH ARTICLE
    Targeted mutagenesis using CRISPR/Cas system in medaka
    Satoshi Ansai, Masato Kinoshita
    Biology Open 2014 3: 362-371; doi: 10.1242/bio.20148177
  • RESEARCH ARTICLE
    Comparison of TALEN scaffolds in Xenopus tropicalis
    Keisuke Nakajima, Yoshio Yaoita
    Biology Open 2013 2: 1364-1370; doi: 10.1242/bio.20136676
  • RESEARCH ARTICLE
    Quantitative assay for TALEN activity at endogenous genomic loci
    Yu Hisano, Satoshi Ota, Kazuharu Arakawa, Michiko Muraki, Nobuaki Kono, Kazuki Oshita, Tetsushi Sakuma, Masaru Tomita, Takashi Yamamoto, Yasushi Okada, Atsuo Kawahara
    Biology Open 2013 2: 363-367; doi: 10.1242/bio.20133871

Other subject collections

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Interviews
  • Sign up for alerts

About us

  • About BiO
  • Editors and Board
  • Editor biographies
  • Grants and funding
  • Journal Meetings
  • Workshops
  • The Company of Biologists

For Authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Article types
  • Manuscript preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access

Journal Info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Sign up for alerts

Contact

  • Contact BiO
  • Advertising
  • Feedback

Twitter   YouTube   LinkedIn

© 2021   The Company of Biologists Ltd   Registered Charity 277992