Microscopy
- Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku
Summary: The authors describe a fluorescently tagged Ku70 protein to monitor replication stress in live S. pombe cells.
- Xenotransplantation of Human glioblastoma in Zebrafish larvae: in vivo imaging and proliferation assessment
Summary: The combination of light sheet fluorescent microscopy and flow cytometry allows assessment of proliferation and tumor growth of human GBM inside zebrafish, making it a useful model to identify effective anti-proliferative agents in a preclinical setting.
- High-throughput live-imaging of embryos in microwell arrays using a modular specimen mounting system
Summary: This Methods & Techniques article describes a customizable device for mounting and live-imaging a wide range of tissues and species; complete design files and instructions for assembly are included.
- Systematic and general method for quantifying localization in microscopy images
Summary: We evaluate a metric for quantifying localization in microscopy images, termed the threshold overlap score, which is simple to calculate and interpret, generally applicable, and suitable for mixed localization patterns.