Mitosis
- Effectors of the spindle assembly checkpoint are confined within the nucleus of Saccharomyces cerevisiae
Summary: The effectors of the spindle assembly checkpoint are confined with the nuclear compartment of budding yeast, and cannot exchange between nuclei in a binucleate zygote.
- Human asparagine synthetase associates with the mitotic spindle
Summary: While yeast Asn1p/ASN2p forms cytoplasmic filaments in response to nutrient limitation, hASNS is associated with centrosomes and mitotic spindles in actively dividing cells, suggesting its additional role in cell division.
- Phosphatase-regulated recruitment of the spindle- and kinetochore-associated (Ska) complex to kinetochores
Summary: Feedback between protein phosphatases and the spindle- and kinetochore-associated (Ska) complex regulates chromosome movement and the metaphase-to-anaphase cell cycle transition. This article has an associated First Person interview with the first author of the paper as part of the supplementary information.
- Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase
Summary: The fission yeast neddylation ligase Dcn1 plays a role in chromosome segregation. dcn1-deletion results in activation of the Mad2-dependent spindle assembly checkpoint, and lagging chromosomes at anaphase.
- Cross-linking mass spectrometry identifies new interfaces of Augmin required to localise the γ-tubulin ring complex to the mitotic spindle
Summary: A map of subunit positional restrictions in Drosophila Augmin shows that Dgt3, Dgt5 and Dgt6 are all required to localise gamma-TuRC to mitotic spindles.
- Quantitative microscopy uncovers ploidy changes during mitosis in live Drosophila embryos and their effect on nuclear size
Summary: A new 3D time-lapse microscopy image analysis pipeline consisting of nuclear segmentation, tracking, annotation and quantification revealed karyotype changes in Drosophila embryos.
- A novel mutation in the N-terminal domain of Drosophila BubR1 affects the spindle assembly checkpoint function of BubR1
Summary: We have identified a novel short motif sequence in the Drosophila BubR1 protein which when mutated affects the amount of Cdc20 recruited to the kinetochores and leads to an abrogation of the SAC.