organoid
- Three-dimensional model of glioblastoma by co-culturing tumor stem cells with human brain organoids
Summary: We describe a method to co-culture glioblastoma stem cells with human cerebral organoids in order to model tumor complexity and recapitulate cellular heterogeneity in vitro.
- WRN conditioned media is sufficient for in vitro propagation of intestinal organoids from large farm and small companion animals
Summary: Culturing methods for the propagation of crypt-derived mouse and human enteroids are applicable to large farm and small companion animals, demonstrating conservation of the crypt signaling microenvironment.
- Simplified 3D protocol capable of generating early cortical neuroepithelium
Summary: We present a simplified method to generate early neuroepithelium of the cerebellar cortex in a 3D culture using defined medium and minimal addition of growth factors.