Proteolysis
- Developing fluorescence sensor probe to capture activated muscle-specific calpain-3 (CAPN3) in living muscle cells
Summary: Our research is the first report of developing sensor probes to detect activated-CAPN3 in cultured skeletal muscle cells.
- Unanchored ubiquitin chains do not lead to marked alterations in gene expression in Drosophila melanogaster
Summary: Our Drosophila studies indicate the lack of a marked, coordinated response towards unanchored poly-ubiquitin in flies, suggesting that untethered ubiquitin chains are not necessarily problematic in intact organisms.
- Influence of a GSK3β phosphorylation site within the proximal C-terminus of Neurofilament-H on neurofilament dynamics
Summary: We demonstrate that phosphorylation of a critical site regulates neurofilament transport, proteolysis and interaction with other axonal cytoskeletal elements, and present evidence that it does so by altering protein conformation. This article has an associated First Person interview with the first author of the paper as part of the supplementary information.
- Twist1-positive epithelial cells retain adhesive and proliferative capacity throughout dissemination
Summary: Twist1+ epithelium retains intercellular junctions and proliferative capacity. During dissemination, Twist1+ cells retain adhesive capacity and exhibit features of both amoeboid and mesenchymal migration modes.
- CLIC1 regulates dendritic cell antigen processing and presentation by modulating phagosome acidification and proteolysis
Summary: DC phagosomes from CLIC1−/− mice display impaired acidification and in vivo and in vitro antigen processing and presentation, revealing CLIC1−/− as a potential therapeutic target in reducing the adaptive immune response in autoimmune diseases.