Reprogramming
- STAT3 modulates reprogramming efficiency of human somatic cells; insights from autosomal dominant Hyper IgE syndrome caused by STAT3 mutations
Summary: By using naturally-occurring mutations in a human disease, we demonstrate the critical role of endogenous STAT3 in modulating reprogramming efficiency in human somatic cells.
- LIF-activated Jak signaling determines Esrrb expression during late-stage reprogramming
Summary: Esrrb is a downstream target and effector of LIF during reprogramming. Forced Esrrb expression accelerates pluripotency establishment in the absence of LIF signaling. The activation of Esrrb is LIF dependent in the reprogramming process.
- In vitro and in vivo differentiation of induced pluripotent stem cells generated from urine-derived cells into cardiomyocytes
Summary: We generated and verified iPS cells from urine-derived cells in a totally noninvasive manner and induced the differentiation of iPS cells into functional cardiomyocytes both in vitro and in vivo.
- Reprogramming towards totipotency is greatly facilitated by synergistic effects of small molecules
Summary: The optimized culture condition with small molecules is sufficient to allow highly efficient mouse cloning by removing epigenetic barriers to reprogramming.
- Systematic evaluation of markers used for the identification of human induced pluripotent stem cells
Summary: The use of hESC-like morphology, retroviral transgene silencing and temporal expression of pluripotency markers are compared as methods to aid in the identification of hiPSC clones.
- Co-expression networks in generation of induced pluripotent stem cells
Summary: We developed a novel adenoviral iPSC reprogramming vector integrating Yamanaka's four factors in a single cassette, allowing for the identification of biologically relevant co-expression networks.
- Generation of primitive neural stem cells from human fibroblasts using a defined set of factors
Summary: A novel method for the generation of self-renewable, multipotent and neural lineage-restricted LIF-dependent induced primitive neural stem cells (LD-iNSCs) from human fibroblasts with the potential to facilitate human neuronal studies.