Zebrafish
- Restriction of mitochondrial calcium overload by mcu inactivation renders a neuroprotective effect in zebrafish models of Parkinson's disease
Summary: Mitochondrial calcium overload causes mitochondrial dysfunction in Parkinson's disease. Inactivation of mitochondrial calcium uniporter reverses mitochondrial calcium overload and rescues dopaminergic neurons in our zebrafish models of Parkinson's disease.
- Failure to identify modifiers of NEBULIN-related nemaline myopathy in two pre-clinical models of the disease
Summary: NEBULIN-related nemaline myopathy currently has no treatment. We attempted to uncover new avenues for therapy by performing modifier screens, which unfortunately failed to identify modifiers that improved disease relevant phenotypes.
- Fgf8a mutation affects craniofacial development and skeletal gene expression in zebrafish larvae
Summary: A function-blocking mutation in fgf8a causes craniofacial malformations in zebrafish larvae due to impaired cranial neural crest cell migration and survival.
- Beyond the whole-mount phenotype: high-resolution imaging in fluorescence-based applications on zebrafish
Summary: We propose a GMA embedding method that preserves fluorescent signals without the need for antibodies. The method complements whole-mount methods especially if advanced imaging technology is not readily available.
- Xenotransplantation of Human glioblastoma in Zebrafish larvae: in vivo imaging and proliferation assessment
Summary: The combination of light sheet fluorescent microscopy and flow cytometry allows assessment of proliferation and tumor growth of human GBM inside zebrafish, making it a useful model to identify effective anti-proliferative agents in a preclinical setting.
- Hedgehog signaling regulates osteoblast differentiation in zebrafish larvae through modulation of autophagy
Summary: Our report of an essential regulation role of hedgehog signaling and autophagy on osteoblast differentiation may contribute to research on bone development biology, hedgehog signaling and the autophagy pathway.
- Prostaglandin E2 promotes embryonic vascular development and maturation in zebrafish
Summary: Overproduction of PGE2 causes inflammation and cancer and its blockage results in serious physiological consequences. This study investigated the novel roles of PGE2 in early vascular development of zebrafish.
- Mutation of microphthalmia-associated transcription factor (mitf) in zebrafish sensitizes for glomerulopathy
Summary: We discovered that the mitf mutation, causative for nacre-albinism mutation in zebrafish, sensitizes for glomerulopathy-induced by puromycin aminonucleoside (PAN) and developed a fish water treatment-model for anti-proteinuric drug screenings.
- An in vivo translation-reporter system for the study of protein synthesis in zebrafish embryos
Summary: We describe a transgene-based method, SPoT, that reveals subcellular sites of protein synthesis in neurons of wholemount zebrafish embryos.
- Basal constriction during midbrain–hindbrain boundary morphogenesis is mediated by Wnt5b and focal adhesion kinase
Summary: Focal adhesion kinase acts downstream of Wnt5b to mediate basal constriction of neuroepithelial cells during the formation of the midbrain–hindbrain boundary.