Zebrafish
- A framework for quantification and physical modeling of cell mixing applied to oscillator synchronization in vertebrate somitogenesis
Summary: We develop a framework to quantify and model cell mixing independent of a choice of reference frames, and apply this to study oscillator synchronization in the zebrafish segmentation clock.
- Senescence gives insights into the morphogenetic evolution of anamniotes
Summary: We report the presence of senescent cells in several transient structures in developing amphibian and teleost fish, suggesting novel mechanisms of morphogenesis that appeared early in vertebrate evolution.
- Parallel in vivo monitoring of pH in gill capillaries and muscles of fishes using microencapsulated biomarkers
Summary: An optical technique for the simultaneous in vivo monitoring of pH in the blood of gill capillaries and interstitial fluid of muscles developed and tested in zebrafish.
- A role for planar cell polarity during early endoderm morphogenesis
Summary: Here we characterise endodermal morphogenesis during somitogenesis stages, and show that PCP signalling plays a mixed role in endoderm development during these stages.
- Disruption of pdgfra alters endocardial and myocardial fusion during zebrafish cardiac assembly
Summary: The molecular mechanisms regulating cardiac fusion are not well understood. Here, we show that platelet-derived growth factor receptor alpha is essential for normal endocardial and myocardial fusion during zebrafish development.
- Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model
Summary: Patient-derived primary cells maintain their original phenotype when cultured on 3D scaffolds, and their in vivo behavior can be effectively studied with innovative zebrafish models.
- Utilising polymorphisms to achieve allele-specific genome editing in zebrafish
Summary: Heterozygous single nucleotide polymorphisms in CRISPR/Cas9 target sites bias genome editing in favour of alleles with perfect complementarity to gRNAs, a feature which can be exploited for chromosome-specific editing.
- Molecular insights into Adgra2/Gpr124 and Reck intracellular trafficking
Summary: This work uncovers molecular determinants of Adgra2/Gpr124 and Reck trafficking to the plasma membrane where the partners meet to act as potent Wnt7-specific Wnt/β-catenin signaling co-activators required for brain vascularization.
- Heterotypic interactions regulate cell shape and density during color pattern formation in zebrafish
Summary: The conspicuous pigmentation pattern of zebrafish is produced by three kinds of interacting pigment cells. Here we address the cellular consequences of these interactions in wild-type fish and mutants with altered pigment patterns.
- αE-catenin-dependent mechanotransduction is essential for proper convergent extension in zebrafish
Summary: Cadherin adhesions transduce tension across the junction-actomyosin axis into proportional biochemical responses via α-catenin. Here we show for the first time how this function of the cadherin complex is important during zebrafish morphogenesis.